Purpose
To propose and evaluate a novel multidimensional approach for imaging sub-voxel tissue compartments called Diffusion-Relaxation Correlation Spectroscopic Imaging (DR-CSI).
Theory and Methods
Multi-exponential modeling of MR diffusion or relaxation data is commonly used to infer the many different microscopic tissue compartments that contribute signal to macroscopic MR imaging voxels. However, multi-exponential estimation is known to be difficult and ill-posed. Observing that this ill-posedness is theoretically reduced in higher dimensions, DR-CSI uses a novel multidimensional imaging experiment that jointly encodes diffusion and relaxation information, and then uses a novel constrained reconstruction technique to generate a multidimensional diffusion-relaxation correlation spectrum for every voxel. The peaks of the multidimensional spectrum are expected to correspond to the distinct tissue microenvironments that are present within each macroscopic imaging voxel.
Results
Using numerical simulations, experiment data from a custom-built phantom, and experiment data from a mouse model of traumatic spinal cord injury, DR-CSI is demonstrated to provide substantially better multi-compartment resolving power compared to conventional diffusion- and relaxation-based methods.
Conclusion
The DR-CSI approach provides powerful new capabilities for resolving the different components of multi-compartment tissue models, and can be leveraged to significantly expand the insights provided by MRI in studies of tissue microstructure.