A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella

Mesureur, Jennifer; Arend, Sandrine; Cellière, Béatrice; Courault, Priscillia; Cotte-Pattat, Pierre-Jean; Totty, Heather; Deol, Parampal; Mick, Virginie; Girard, Victoria; Touchberry, Joanne; Burrowes, Vanessa; Lavigne, Jean‐Philippe; O’Callaghan, David; Monnin, Valérie; Keriel, Anne

doi:10.1371/journal.pntd.0006874

Cited by 48 publications

(36 citation statements)

References 37 publications

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“…MALDI-TOF-MS is used in developed provincial CDCs and is becoming the method of choice for bacterial identification in some modern diagnostic laboratory. A new spectral database has been developed for the bioMerieux Vitek system which allows the identification of Brucella at the species level [15].…”

Section: Clinical Diagnosismentioning

confidence: 99%

Brucellosis in China: history, progress and challenge

2020

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Brucellosis is a neglected zoonosis. It causes acute febrile illness and a potentially debilitating chronic infection in humans, and livestock infection has substantial socioeconomic impact. Over the past two decades, improvements have been made to better understand the various aspects of human and animal brucellosis. Meanwhile, especially in the developing world, immense challenges that remain in controlling and eradicating brucellosis are novel diagnostics tools and efficacious vaccines. Here, we will focus on the remarkable issues on epidemiological survey, as well as the priority and challenge of brucellosis in China. Brucellosis will be controlled with meaningful collaboration between local and public partnerships effectively applying a One Health framework.

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Section: Clinical Diagnosismentioning

confidence: 99%

Brucellosis in China: history, progress and challenge

2020

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“…MALDI-TOF mass spectrometry is revolutionizing the clinical diagnostic laboratory, but not all machines can identify Brucella. We developed a spectral database allowing Brucella to be identified by the bioMérieux VITEK system [7] and a safe, rapid protocol for solvent inactivation before analysis. Solvent inactivated bacteria are stable for several days, allowing transport to a centre with a machine [8].…”

Section: Strain Identification and Molecular Epidemiologymentioning

confidence: 99%

Human brucellosis: recent advances and future challenges

O’Callaghan

2020

Infect Dis Poverty

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Bacteria of the genus Brucella cause brucellosis, one of the world's neglected zoonotic diseases. It is a disease of poverty; infections of livestock have a huge socioeconomic cost while human brucellosis starts as a debilitating acute infection that can be come chronic with many complications. The figure of 500 000 new human cases each year is regularly cited in reviews and research papers, however this is a vast under estimation; many of the most affected countries do not have the infrastructure for diagnosis and the broad spectrum of symptoms are shared with other febrile infections. Considering the importance of the disease, the international brucellosis research community is small; we cover a wide range of research interests ranging from veterinary and human medicine to molecular genetics, cell biology and immunology. Much work is centred on the deciphering how Brucella cause disease in animals and man; how they enter and survive in host cells, how they use their VirB Type IV secretion system to deliver effectors that modulate host cell biology and immune response.. This will lead to the identification of new strategies for vaccine development, targets for diagnostic and prognostic tests and the development of new therapies. However, as noted in the first few lines of this editorial, brucellosis is a disease of poverty; and it is important that, especially for disease surveillance, diagnosis, control and treatment, that we work for solutions that are relevant and applicable to the countries where the disease is really a problem. Here I will list a few points that highlight a small selection of the recent practical advances and some of the major challenges.

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“…The MALDI-TOF MS (Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) assay is an advanced tool for rapid and accurate identification of microorganisms. This technique has been widely applied to identify bacteria, yeasts and other fungi (Pinto et al, 2011;Mesureur et al, 2018;Pauker et al, 2018;Quero et al, 2019;Kim et al, 2019), especially for the identification of the human pathogens, whereas might be relatively less popular in the plant pathogen research at this moment. However, a broader application can be foresee in the near future based on its excellent accuracy and efficiency, and fast development of analyzing equipment.…”

Section: Identification Of Fusarium Speciesmentioning

confidence: 99%

The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

Cen

Lin

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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In recent years, there has been a noticeable increase in research interests on the Fusarium species, which includes prevalent plant pathogens and human pathogens, common microbial food contaminants and industrial microbes. Taken the advantage of gibberellin synthesis, Fusarium fujikuroi succeed in being a prevalent plant pathogen. At the meanwhile, F. fujikuroi was utilized for industrial production of gibberellins, a group of extensively applied phytohormone. F. fujikuroi has been known for its outstanding performance in gibberellin production for almost 100 years. Research activities relate to this species has lasted for a very long period. The slow development in biological investigation of F. fujikuroi is largely due to the lack of efficient research technologies and molecular tools. During the past decade, technologies to analyze the molecular basis of host-pathogen interactions and metabolic regulations have been developed rapidly, especially on the aspects of genetic manipulation. At the meanwhile, the industrial fermentation technologies kept sustained development. In this article, we reviewed the currently available research tools/methods for F. fujikuroi research, focusing on the topics about genetic engineering and gibberellin production.

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A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella

Cited by 48 publications

References 37 publications

Brucellosis in China: history, progress and challenge

Brucellosis in China: history, progress and challenge

Human brucellosis: recent advances and future challenges

The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

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