A Mammary Stem Cell Population Identified and Characterized in Late Embryogenesis Reveals Similarities to Human Breast Cancer

Spike, Benjamin T.; Engle, Dannielle D.; Lin, Jennifer; Cheung, Samantha K.; La, Justin; Wahl, Geoffrey M.

doi:10.1016/j.stem.2011.12.018

Cited by 203 publications

(308 citation statements)

References 69 publications

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“…Several lines of evidence indicate that mammary glands have evolved from sweat glands (Jenness, 1974;Long, 1969;Oftedal, 2002). We found that sweat gland stem cells share many features with mammary stem cells Spike et al, 2012;Stingl et al, 2006). Similar to mammary stem cells, sweat gland stem cells predominantly reside among myoepithelial cells and are capable of generating exocrine gland-like hollow spheres in vitro.…”

Section: Discussionmentioning

confidence: 81%

“…Suspension sphere cultures were performed according to Spike et al (2012) with slight modification. Freshly sorted cells were plated on ultralow-adherence plates (Corning, Corning, NY) at 1000 cells/cm 2 in sphere culture medium (Complete MammoCult Human Medium containing 0.5 μg/ml hydrocortisone 21-hemisuccinate (Sigma), 10 ng/ml recombinant human epidermal growth factor (PeproTech, Rocky Hill, NJ), 10 ng/ml recombinant human basic fibroblast growth factor (Stem Cell Technologies), 4 μg/ml heparin (Stem Cell Technologies), and 100 μg/ml penicillin/streptomycin (Gibco)) with or without 2% Matrigel (growth factor-reduced; BD Biosciences).…”

Section: In Vitro Sphere Culturesmentioning

confidence: 99%

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Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin

Kurata

Futaki

Nakano

et al. 2014

Cell Struct. Funct.

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ABSTRACT. Stem cells routinely maintain the main epidermal components, i.e. the interfollicular epidermis, hair follicles, and sweat glands. Human sweat glands present throughout the body are glandular exocrine organs that mainly play a role in thermoregulation by sweating. Emerging evidence points to the presence of stem cells in sweat glands, but it remains unclear whether such stem cells exist in human sweat glands. Here, we attempted to gather evidence for stem cells in human sweat glands, which would be characterized by self-renewal ability and multipotency. First, we explored human sweat gland cells for expression of stem cell markers. CD29 and Notch, epidermal stem cell markers, were found to reside among α-smooth muscle actin-positive myoepithelial cells in human sweat glands. Next, sweat gland myoepithelial cells were isolated from human skin as a CD29

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Section: Discussionmentioning

confidence: 81%

Section: In Vitro Sphere Culturesmentioning

confidence: 99%

Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin

Kurata

Futaki

Nakano

et al. 2014

Cell Struct. Funct.

View full text Add to dashboard Cite

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“…The demonstration that the mammary gland contains different types of long-lived stem cells has profound implications for our understanding of mammary gland physiology and will be instrumental in unraveling the cells at the origin of breast cancers [116]. The invasive and proliferative processes of mammogenesis in the fetal mammary stem cell state resemble phases of cancer progression [117].…”

Section: Estrogen Signaling In Breast Cancer Stem Cellsmentioning

confidence: 99%

RETRACTED ARTICLE: Regulation of estrogen receptor signaling in breast carcinogenesis and breast cancer therapy

Zhou

Qiao

Shetty

et al. 2013

Cell. Mol. Life Sci.

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Estrogen and estrogen receptors (ERs) are critical regulators of breast epithelial cell proliferation, differentiation, and apoptosis. Compromised signaling vis-à-vis the estrogen receptor is believed © Springer Basel 2013 yow4@pitt.edu. ), which predicts for response to endocrine-based therapy; however, innate or acquired resistance to endocrinebased drugs remains a serious challenge. The complexity of regulation for estrogen signaling coupled with the crosstalk of other oncogenic signaling pathways is a reason for endocrine therapy resistance. Alternative strategies that target novel molecular mechanisms are necessary to overcome this current and urgent gap in therapy. A thorough analysis of estrogen-signaling regulation is critical. In this review article, we will summarize current insights into the regulation of estrogen signaling as related to breast carcinogenesis and breast cancer therapy. NIH Public Access

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“…Recent studies of the developing mouse mammary gland have revealed that co-expression of CK14 and CK18 occurs in mammary stem cells during embryogenesis, but not subsequently in the adult gland (30,31); such cells can be detected, however, in mouse mammary tumors (32). In the adult human breast, progenitor and stem cell-enriched subpopulations appear to contain cells that express bi-lineage cytokeratins (7,33).…”

Section: Bi-lineage Cells Possess Increased Colony Formation Capacitymentioning

confidence: 99%

EZH2 promotes a bi-lineage identity in basal-like breast cancer cells

et al. 2012

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The mechanisms regulating breast cancer differentiation state are poorly understood. Of particular interest are molecular regulators controlling the highly aggressive and poorly differentiated traits of basal-like breast carcinomas. Here we show that the Polycomb factor EZH2 maintains the differentiation state of basal-like breast cancer cells, and promotes the expression of progenitorassociated and basal-lineage genes. Specifically, EZH2 regulates the composition of basal-like breast cancer cell populations by promoting a "bi-lineage" differentiation state, in which cells co-express basal-and luminal-lineage markers. We show that human basal-like breast cancers contain a subpopulation of bi-lineage cells, and that EZH2-deficient cells give rise to tumors with a decreased proportion of such cells. Bi-lineage cells express genes that are active in normal luminal progenitors, and possess increased colony formation capacity, consistent with a primitive differentiation state. We found that GATA3, a driver of luminal differentiation, performs a function opposite to EZH2, acting to suppress bi-lineage identity and luminal-progenitor gene expression. GATA3 levels increase upon EZH2 silencing, mediating a decrease in bi-lineage cell numbers. Our findings reveal a novel role for EZH2 in controlling basal-like breast cancer differentiation state and intra-tumoral cell composition.

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A Mammary Stem Cell Population Identified and Characterized in Late Embryogenesis Reveals Similarities to Human Breast Cancer

Cited by 203 publications

References 69 publications

Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin

Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin

RETRACTED ARTICLE: Regulation of estrogen receptor signaling in breast carcinogenesis and breast cancer therapy

EZH2 promotes a bi-lineage identity in basal-like breast cancer cells

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