A Map of Olfactory Representation in the Drosophila Mushroom Body

Lin, Hui-Hao; Lai, Jason Sih-Yu; Chin, An‐Lun; Chen, Yung‐Chang; Chiang, Ann‐Shyn

doi:10.1016/j.cell.2007.03.006

Cited by 217 publications

(247 citation statements)

References 53 publications

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“…The topographic map in the antennal lobe, of at least D. melanogaster, is retained in the higher olfactory centres, the mushroom body and the lateral horn of the protocerebrum (Jefferies et al 2007, Lin et al 2007, Marin et al 2002, Wong et al 2002. Projection neurons that innervate a given glomerulus display stereotypic branching patterns in these centres, whereas projection neurons innervating different glomeruli exhibit very different arborisation patterns.…”

Section: Anatomy and Function Of Higher Olfactory Centresmentioning

confidence: 99%

“…Stereotypic connectivity maps of odorant-to-OR , ORN-to-PN (Couto et al 2005, Fishilevich andVosshall 2005), and PN-to-Kenyon cell, the principal neuron type of the mushroom body (Jefferies et al 2007, Lin et al 2007) have allowed the construction of neural computation of odour discrimination in the D. melanogaster brain. Stereotypic PN-to-KC connectivity and functional imaging suggest that differential representation of the odours in the AL is maintained in the MB calyx and possibly further processed in the different MB neurons (Jefferies et al 2007, Lin et al 2007. The distribution of odour responses across different classes of Kenyon cells and the imposition of odor-sensitive excitatory and inhibitory responses both appear to enhance distinct neural representations of different odours.…”

Section: Anatomy and Function Of Higher Olfactory Centresmentioning

confidence: 99%

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Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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Section: Anatomy and Function Of Higher Olfactory Centresmentioning

confidence: 99%

Section: Anatomy and Function Of Higher Olfactory Centresmentioning

confidence: 99%

Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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“…In Drosophila adults, apparently stereotypic projections of PNs and KCs have been defined anatomically only at the level of broad zones (10)(11)(12), and odors can evoke localized PN activity in large regions of the calyx (13). However, at least some subsets of KCs show apparently nonstereotypic responses to odors (9).…”

mentioning

confidence: 99%

Localized olfactory representation in mushroom bodies of Drosophila larvae

Masuda-Nakagawa

Gendre

O’Kane

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Odor discrimination in higher brain centers is essential for behavioral responses to odors. One such center is the mushroom body (MB) of insects, which is required for odor discrimination learning. The calyx of the MB receives olfactory input from projection neurons (PNs) that are targets of olfactory sensory neurons (OSNs) in the antennal lobe (AL). In the calyx, olfactory information is transformed from broadly-tuned representations in PNs to sparse representations in MB neurons (Kenyon cells). However, the extent of stereotypy in olfactory representations in the calyx is unknown. Using the anatomically-simple larval olfactory system of Drosophila in which odor ligands for the entire set of 21 OSNs are known, we asked how odor identity is represented in the MB calyx. We first mapped the projections of all larval OSNs in the glomeruli of the AL, and then followed the connections of individual PNs from the AL to different calyx glomeruli. We thus established a comprehensive olfactory map from OSNs to a higher olfactory association center, at a single-cell level. Stimulation of single OSNs evoked strong neuronal activity in 1 to 3 calyx glomeruli, showing that broadening of the strongest PN responses is limited to a few calyx glomeruli. Stereotypic representation of single OSN input in calyx glomeruli provides a mechanism for MB neurons to detect and discriminate olfactory cues.calyx ͉ genetically-encoded calcium indicator ͉ olfactory sensory neurons ͉ projection neurons W e now understand much about how odor information is detected and conveyed to the brain by sensory neurons, but less about how this information is represented in higher brain centers to influence behavioral outputs. The mushroom body (MB) of insects, which in Drosophila is essential for odor discrimination learning, provides a model to understand olfactory coding in higher association centers (1, 2). In the periphery, odor identity is detected by sets of olfactory sensory neurons (OSNs) whose specificities are determined by the olfactory receptor (OR) that they express (3, 4). OSNs that express the same OR converge on defined glomeruli in the first olfactory center of the brain, the antennal lobe (AL), analogously to the convergence of OSNs on olfactory bulb glomeruli in mammals. Projection neurons (PNs) then carry olfactory information from single AL glomeruli to the higher brain, the MB, and the lateral horn. However, excitatory interneurons that innervate multiple AL glomeruli lead to broadening of PN specificity compared with OSNs (5, 6). PNs then connect to Kenyon cells (KCs) in the calyx of the MB, where the representation of odor qualities is radically transformed; individual KCs respond much more selectively to odors than do either OSNs or PNs (7-9).The extent of stereotypy in olfactory processing in the calyx has been a matter of debate, in contrast to the clearly stereotypic connections between OSNs and PNs in the AL. In Drosophila adults, apparently stereotypic projections of PNs and KCs have been defined anatomically only at the leve...

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“…One of the most informative approaches to this goal is the sparse labelling method that involves visualization of a single neuron per brain and the integration of information across many brain samples; this method has been successfully employed in the Drosophila olfactory system [14][15][16][17] . Among vertebrates, a promising model organism for connectivity mapping is the zebrafish because of its amenability to various genetic techniques, small brain and transparency at early larval stages suitable for high-resolution imaging 18,19 .…”

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confidence: 99%

Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling

et al. 2014

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Chemotopic odour representations in the olfactory bulb are transferred to multiple forebrain areas and translated into appropriate output responses. However, a comprehensive projection map of bulbar output neurons at single-axon resolution is lacking in vertebrates. Here we unravel a projectome of the zebrafish olfactory bulb through genetic single-neuron tracing and image registration. We show that five major target regions receive distinct modes of projections from olfactory bulb glomeruli. The central portion of posterior telencephalon receives non-selective, interspersed inputs from all glomeruli, whereas the ventral telencephalon is diffusely innervated by axons from particular glomerular clusters. The right habenula and posterior tuberculum (diencephalic nuclei) receive convergent inputs from restricted and all glomerular clusters, respectively. The bulbar recurrent projections are coarsely topographic. Thus, the primary chemotopic organization is transformed into distinct sensory representations in higher olfactory centres. These findings provide a framework to understand general principles as well as species-specific features in decoding of odour information.

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A Map of Olfactory Representation in the Drosophila Mushroom Body

Cited by 217 publications

References 53 publications

Olfaction in vector-host interactions

Olfaction in vector-host interactions

Localized olfactory representation in mushroom bodies of Drosophila larvae

Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling

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