A marine lipopeptides-producing Bacillus amyloliquefaciens HY2-1 with a broad-spectrum antifungal and antibacterial activity and its fermentation kinetics study

Huang, Linru; Ling, Xueying; Peng, Shuaiying; Tan, Minghui; Yan, Lu-qi; Liang, Yingyin; Li, Gang-hui; Li, Kuntai

doi:10.1007/s11274-023-03643-y

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…Bacillus amyloliquefaciens ( B. amyloliquefaciens ) is one of the probiotic species with increasing interest, as this species produces spores that can withstand stress; harsh production, storage, and transport environments; and gastric transit. , Previous studies have shown that the supplementation with Bacillus amyloliquefaciens substantially relieved colitis in mice, which reduced inflammation and enhanced the epithelial barrier. , Hou et. al discovered that Bacillus subtilis significantly enhanced the amount of ISCs in colitis mice .…”

Section: Introductionmentioning

confidence: 99%

Bacillus amyloliquefaciens SC06 Relieving Intestinal Inflammation by Modulating Intestinal Stem Cells Proliferation and Differentiation via AhR/STAT3 Pathway in LPS-Challenged Piglets

Wang,

Zhou

et al. 2024

J. Agric. Food Chem.

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Bacillus amyloliquefaciens is a well-accepted probiotic, with many benefits for both humans and animals. The ability of intestinal stem cells (ISCs) to develop into several intestinal epithelial cell types helps accelerate intestinal epithelial regeneration. Limited knowledge exists on how bacteria regulated ISCs proliferation and regeneration. Our study investigated the effects of Bacillus amyloliquefaciens supplementation on ISC proliferation and regeneration and intestinal mucosal barrier functions in piglets exposed to lipopolysaccharide (LPS). Eighteen piglets (male, 21 days old) were randomly split into 3 clusters: CON cluster, LPS cluster, and SC06+LPS cluster. On day 21, 100 μg/kg body weight of LPS was intraperitoneally administered to the SC06+LPS and LPS groups. We found SC06 supplementation maintained the intestinal barrier integrity, enhanced intestinal antioxidant capacity, reduced generation of inflammatory response, and suppressed enterocyte apoptosis against the deleterious effects triggered by LPS. In addition, our research indicated that the SC06 supplementation not only improved the ISC regeneration, but also resulted in upregulation of aryl hydrocarbon receptor (AhR) in LPS-challenge piglets. Further studies showed that SC06 also induced ISC differentiation toward goblet cells and inhibited their differentiation to intestinal absorptive cells and enterocytes. The coculture system of SC06 and ileum organoids revealed that SC06 increased the growth of ISCs and repaired LPS-induced organoid damage through activating the AhR/STAT3 signaling pathway. These findings showed that SC06, possibly through the AhR/STAT3 pathway, accelerated ISC proliferation and promoted epithelial barrier healing, providing a potential clinical treatment for IBD. Our research demonstrated that SC06 is effective in preventing intestinal epithelial damage after pathological injury, restoring intestinal homeostasis, and maintaining intestinal epithelial regeneration.

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Section: Introductionmentioning

confidence: 99%

Bacillus amyloliquefaciens SC06 Relieving Intestinal Inflammation by Modulating Intestinal Stem Cells Proliferation and Differentiation via AhR/STAT3 Pathway in LPS-Challenged Piglets

Wang,

Zhou

et al. 2024

J. Agric. Food Chem.

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“…These are known to inhibit the growth of fungi and bacteria or induce systemic resistance in host plants [11][12][13][14]. Polyketides and other antibiotics (bacilysin and bacteriocin) exhibit strong bactericidal activity against fungi, bacteria, and algae [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…These are known to inhibit the growth of fungi and bacteria or induce systemic resistance in host plants [ 11 , 12 , 13 , 14 ]. Polyketides and other antibiotics (bacilysin and bacteriocin) exhibit strong bactericidal activity against fungi, bacteria, and algae [ 15 , 16 , 17 , 18 , 19 ]. Antimicrobial substances synthesized through the ribosomal pathway mainly include bacteriocins (subtilin, sublancin 168, and subtilosin), hydrolytic enzymes (e.g., extracellular amylase, chitinase, and glucanases), and other unidentified antimicrobial proteins [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Genome and Transcriptome Analysis to Elucidate the Biocontrol Mechanism of Bacillus amyloliquefaciens XJ5 against Alternaria solani

et al. 2023

Microorganisms

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Early blight, caused by Alternaria solani, is an important disease affecting tomatoes. Biological control offers an environmentally friendly approach to controlling pathogens. Herein, we identified a B. amyloliquefaciens strain XJ5 and investigated its biocontrol mechanism against A. solani. A. solani growth was significantly inhibited by XJ5, with the inhibition rate of cell-free culture supernatants reaching 82.3%. Furthermore, XJ5 crude protein extracts inhibited conidia germination and altered the mycelial morphology of A. solani. To uncover the potential biocontrol mechanism of XJ5, we analyzed its genome sequence and transcriptome. The genome of XJ5 comprised a 4.16 Mb circular chromosome and two circular plasmids. A total of 13 biosynthetic gene clusters and 127 genes encoding hydrolases were identified, suggestive of the ability of XJ5 to secrete antagonistic secondary metabolites and hydrolases. Transcript analysis revealed 174 differentially expressed genes on exposing A. solani to XJ5 crude protein extracts. The expression of genes related to chitin and mannose synthesis was downregulated, indicating that XJ5 metabolites may impact chitin and mannose synthesis in A. solani. Overall, these findings enhance our understanding of the interactions between B. amyloliquefaciens and phytopathogens and pave the way for the agricultural application of this promising biocontrol agent.

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Antifungal lipopeptides from the marine Bacillus amyloliquefaciens HY2–1: A potential biocontrol agent exhibiting in vitro and in vivo antagonistic activities against Penicillium digitatum

Liao,

Xu,

Wang

et al. 2025

International Journal of Biological Macromolecules

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A marine lipopeptides-producing Bacillus amyloliquefaciens HY2-1 with a broad-spectrum antifungal and antibacterial activity and its fermentation kinetics study

Cited by 7 publications

References 56 publications

Bacillus amyloliquefaciens SC06 Relieving Intestinal Inflammation by Modulating Intestinal Stem Cells Proliferation and Differentiation via AhR/STAT3 Pathway in LPS-Challenged Piglets

Bacillus amyloliquefaciens SC06 Relieving Intestinal Inflammation by Modulating Intestinal Stem Cells Proliferation and Differentiation via AhR/STAT3 Pathway in LPS-Challenged Piglets

Genome and Transcriptome Analysis to Elucidate the Biocontrol Mechanism of Bacillus amyloliquefaciens XJ5 against Alternaria solani

Antifungal lipopeptides from the marine Bacillus amyloliquefaciens HY2–1: A potential biocontrol agent exhibiting in vitro and in vivo antagonistic activities against Penicillium digitatum

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