A marker of oxidative stress in saliva: association with periodontally-involved teeth of a hopeless prognosis

Abstract

The aim of this study was to determine the association between levels of a marker of oxidative stress, 8-hydroxydeoxyguanosine (8-OHdG), in saliva and the presence of teeth with a hopeless prognosis as a result of advanced periodontitis. Thirty-four periodontitis patients were divided into two groups based on the presence or absence of periodontally-involved teeth of hopeless prognosis. Salivary levels of 8-OHdG in those with were significantly higher than in subjects without periodontally-involved teeth of hopeless prognosis (4.78 +/- 0.14 ng/ml and 2.35 +/- 0.18 ng/ml, respectively). We also evaluated 8-OHdG levels in gingival crevicular fluid (GCF) of teeth with advanced periodontal destruction (mean probing depth = 7.2). In this case, 8-OHdG was detected only from those periodontally-involved teeth of hopeless prognosis, and only in some cases (8 out of 18 samples). These data suggest that periodontally-involved teeth of hopeless prognosis are a major source of salivary 8-OHdG. Measurement of salivary 8-OHdG levels may prove to be useful in identifying patients with teeth of hopeless prognosis.

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

“…Our study showed that the salivary 8-OHdG levels were significantly higher in the periodontitis patients than in the healthy controls. This result confirms findings of limited previous studies (Sawamoto et al 2005;Takane et al 2005;Takane et al 2002). Also, we detected the 5-kbp mtDNA deletion in the gingival tissues of 20 of the 32 periodontitis patients and did not detect the 7-kbp mtDNA deletion in the periodontitis patients.…”

“…However, information on markers of ROS reactions with DNA in periodontitis is limited. The majority of published data on oxidative damage to DNA has been reported by a Japanese group who investigated 8-OHdG levels in the saliva of periodontitis patients (Sawamoto et al 2005;Takane et al 2005;Takane et al 2002). Their studies demonstrated that salivary levels of 8-OHdG in samples from subjects with CP were significantly higher than those from periodontally healthy controls.…”

Ability of Orally Administered IFN-.ALPHA.4 to Inhibit Naturally Occurring Gingival Inflammation in Dogs

“…In the present study, we evaluated the levels of 8-OHdG [36] and HEL [20], which are both oxidative stress markers, in whole saliva as biomarkers in clinically healthy individuals (Group 1). Both 8-OHdG and HEL decreased significantly after LDOA of CaIFN-4 In Group 1, 4 of 5 dogs tested positive for occult blood, but this rate decreased during LDOA of CaIFN-4 (data not shown), possibly reflecting stress levels.…”

“…The saliva samples of the dogs in Group 1 were centrifuged at 10,000  g for 10 min, and the supernatant was used to determine 8-hydroxydeoxyguanosine (8-OHdG) and N-(Hexanoyl) Lysine (HEL) levels as oxidative stress biomarkers [36,20] with enzyme-linked immunosorbent assay (ELISA) kits (Japan Institute for the Control of Aging, Nikken SEIL Corporation, Fukuroi, Shizuoka, Japan).…”

Nuclear Factor Erythroid 2-Related Factor 2 Down-Regulation in Oral Neutrophils Is Associated with Periodontal Oxidative Damage and Severe Chronic Periodontitis

“…One study reported high 8-OHdG levels in whole saliva and GCF of patients with CP in a Japanese population, but systemic levels of 8-OHdG were not measured. 42 However, GCF levels of 8-OHdG were suggested as a biomarker for DNA damage caused by oxidative stress and an indicator for CP severity. 43 Konopka et al 44 showed that 8-OHdG levels in Figure 4 In response to ligature-biofilms, nuclear factor erythroid 2-related factor 2 (Nrf2 À/À ) mice develop severe bone and attachment loss associated with high oxidative stress.…”