A Mathematical Model Describing Tannin–Protein Association

Silber, M.; Davitt, Bruce B.; Khairutdinov, R. F.; Hurst, James K.

doi:10.1006/abio.1998.2817

Cited by 19 publications

(9 citation statements)

References 17 publications

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“…However, when rutin was further increased to 9 mg/g dry gelatin, there was granule precipitate in hydrogels, which could also be found in previous studies (Naczk, Grant, Zadernowski, & Barre, 2006). Silber, Davitt, Khairutdinov, and Hurst (1998) postulated that protein could be precipitated by polyphenols when the number of polyphenol molecules interacting with a protein molecule reached a critical value. The gel strength of modified hydrogel decreased evidently after inclusion of 30 mg/g dry gelatin of gallic acid, the reason of which might be the formation of a sufficient coating of protein surfaces by polyphenol molecules (Charlton et al, 2002).…”

Section: Gel Strengthsupporting

confidence: 75%

Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin

Yan

Zhao

et al. 2011

Food Hydrocolloids

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Section: Gel Strengthsupporting

confidence: 75%

Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin

Yan

Zhao

et al. 2011

Food Hydrocolloids

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“…Figure 3 shows that the slopes of titration curves are affected by the concentrations of both BSA and dye-labeled BSA in the solutions used for the determination of titration curves. The relationship between the amount of SCT-protein complex precipitated and the quantity of proteins added to a solution containing a known amount of SCT is described by a bell-shaped curve (15)(16)(17). The maximal precipitation of tannin-protein complex occurs at the optimal tannin-to-protein ratio referred to as the equivalence point (16).…”

Section: Resultsmentioning

confidence: 99%

“…The maximal precipitation of tannin-protein complex occurs at the optimal tannin-to-protein ratio referred to as the equivalence point (16). Excess protein leads to the formation of soluble tannin-protein complexes (16), as the precipitation of tannin-protein complex occurs when the number of tannin molecules associated with a protein molecule reaches a critical value (17). Therefore, the changes in the S p -and S d -slope values indicate that at protein contents ≤1 mg per mL (for PPA) and ≤4 mg per mL (for DPLA); there was an excess of SCT in the reaction mixture.…”

Section: Resultsmentioning

confidence: 99%

Protein‐precipitating capacity of crude condensed tannins of canola and rapeseed hulls

Naczk

Amarowicz

Zadernowski

et al. 2001

J Americ Oil Chem Soc

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The protein-precipitating potentials (PPP) of soluble condensed tannins (SCT) were determined in hulls from several samples of canola and rapeseed varieties. The PPP were expressed as slopes of lines (titration curves) reflecting the amount of SCT-protein precipitated vs. the amount of SCT added to the reaction mixture. The slopes (S p ) of titration curves obtained using the protein-precipitation assay ranged from 2.96 to 10.91 (absorbance units at 510 nm per mg SCT), and those of titration curves, obtained using the dye-labeled bovine serum albumin (BSA) assay (S d ), ranged from 28.1 to 267 (% precipitated BSA per mg SCT). For both assays, a statistically significant (P ≤ 0.001) semilogarithmic linear correlation existed between the slopes and the SCT contents in the canola and rapeseed hulls. Higher amounts of SCT-protein complexes were precipitated at 40°C than at room temperature. Determination of titration curves under standardized conditions (type and concentration of protein, pH and temperature) afforded meaningful differences in the slopes among the range of SCT extracts from canola and rapeseed hulls used in this study. FIG. 3. Effect of protein concentrations on the slope values (S p and S d ) of titration curves obtained by using (A) PPA and (B) DLPA. SCT were extracted from Cyclone canola hulls, sample 5. For symbols and abbreviations see Figures 1 and 2.

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“…These tannin fractions possess a well known ability to bind to proteins (leading to the formation of large protein aggregates) and precipitate (Naczk et al, 2001;Silber et al, 1998), properties that could affect both the enzymatic activity and the DNA replication by the formation of crosslinks with required proteins. Literature data also support that tannic acid and its hydrolyzed products lack mutagenic activity in Salmonella tester strains (Chen, Chung, 2000).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and genotoxic investigation on barbatimão [Stryphnodendron adstringens (Mart: ) Coville, 1910] extract

Vilar

D'Oliveira

Santos

et al. 2010

Braz. J. Pharm. Sci.

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Stryphnodendron adstringens (Mart.) Coville, 1910 is a small tree, distributed widely throughout the Cerrado region of Brazil and named "barbatimão" by the Tupi-Guarani tribes, which presents astringent properties. Its ethnopharmacological uses comprise, among others, anti-inflammatory and wound healing action, and it is used in the treatment of diarrhea and gynecological problems. The phytotherapeutic use of 'barbatimão' is largely related to its tannin content, which is abundant in its bark. The main goal of the present study was to evaluate the cytotoxic, mutagenic, and genotoxic potential of the lyophilized solution of the stem bark of S. adstringens, using the Ames test, the SOS-Inductest and the SOSChromotest. S. adstringens presented cytotoxic activity in all tested systems, did not present mutagenic activity detectable by the Ames test and SOS-Chromotest, and showed some genotoxic effect on the SOS-Inductest. However, the metabolization of the extract by S9 fraction attenuated its genotoxic and cytotoxic activities. Uniterms:Stryphnodendron adstringens/cytotoxicity. Stryphnodendron adstringens/genotoxicity. Barbatimão/phytochemical study.Stryphnodendron adstringens (Mart.) Coville, 1910 é uma pequena árvore amplamente distribuída nas regiões de cerrado do Brasil, chamada de "barbatimão" pelas tribos Tupi-Guarani, que apresenta propriedade adstringente. Seu uso etnofarmacológico compreende, entre outros, efeitos antiinflamatório e cicatrizante, sendo empregada no tratamento de diarréias e problemas ginecológicos. Grande parte das aplicações do fitoterápico de barbatimão está relacionada aos taninos, abundantes em sua casca. O objetivo do presente trabalho foi avaliar os potenciais citotóxico, mutagênico e genotóxico da solução liofilizada da casca de S. adstringens, utilizando Teste de Ames, SOS-Induteste e SOS-Cromoteste. S. adstringens apresentou atividade citotóxica em todos os sistemas testados, não apresentou atividade mutagênica detectável pelo teste de Ames e SOS-Cromoteste e mostrou certo efeito genotóxico no SOS-Induteste. Porém, a metabolização do extrato pela fração S9 atenuou suas atividades genotóxica e citotóxica. Unitermos: Stryphnodendron adstringens/citotoxicidade. Stryphnodendron adstringens/genotoxicidade.Stryphnodendron adstringens/mutagênese. Barbatimão/estudo fitoquímico.

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A Mathematical Model Describing Tannin–Protein Association

Cited by 19 publications

References 17 publications

Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin

Physicochemical properties of gelatin gels from walleye pollock (Theragra chalcogramma) skin cross-linked by gallic acid and rutin

Protein‐precipitating capacity of crude condensed tannins of canola and rapeseed hulls

Cytotoxic and genotoxic investigation on barbatimão [Stryphnodendron adstringens (Mart: ) Coville, 1910] extract

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