A mathematically designed STS primer without any mismatches for direct sequencing of cosmid DNA clones

Tang, Xiaoou; Wang, Yifei; Nakata, Yasuhiko; Li, Haiou; Fujita, Akiko; Gao, Hui; Sarai, Akinori; Yokoyama, Kazushige

doi:10.1007/bf01907984

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Then, 13 positive clones were obtained. We confirmed that one of cosmid clones (noo 8) was the correct functional clone corresponded to a PCR-3 clone by nucleotide sequencing as described by Tang et al (1993) (data not shown; Amakawa et al, 1993;Brou et al, 1994;Hamaguchi et al, t989;Matsunami et al, 1989). A cosmid clone (no.…”

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confidence: 77%

Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescencein situ hybridization

et al. 1997

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SummaryThe transcription factor KBF2 has been characterized as a factor that binds to the NFkB site of mouse major histocompatibility complex (MHC) class I genes and its amino acid sequence has been shown to be identical to those of members of the recombination signalsequence binding protein (RBP-Jk) family. Previous studies by Amakawa et al. (Genomics 17, 306-315, 1993) demonstrated that the functional gene is localized at human chromosome 3q25. However, in the present study we showed by in situ hybridization With the functional KBF2/ RBPJk cosmid clone that the gene is localized at 9p12-13 and 9q13, namely, at the same loci as pseudogenes that were reported previously (Zhang et al., Jpn J Human Genet 39, 391-401, 1994). Key Words KBF2, RBP-Jk, cosmid, in situ hybridizationThe major histocompatibility complex (MHC) class I antigens specified by MHC class I genes play a key role in a number of immunological processes, particularly in the recognition of foreign antigens by cytotoxic T cells and natural killer ceils of the host (Zinkernagel and Doherty, 1979). In the mouse, the promoters of the K b and L d class I MHC genes have been used as models in studies of transcription (Israel et al., 1989). A factor designated KBF2, which can bind as a monomer to conserved sequence element (TGGGGATTCCCCA) located 165 bp upstream of the initiation site of the H-2K b class I gene. Interestingly, the same

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confidence: 77%

Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescencein situ hybridization

et al. 1997

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“…The cosmid cloned DNAs were sequenced directly (Slightom and Sieu, 1992) by a modified method as described by Tang et al (1993).…”

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confidence: 99%

Genomic structure and chromosomal localization of processed pseudogenes for human RBP-Jk

Zhang

Tang²,

Jin

et al. 1994

Jap J Human Genet

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SummaryThe functional gene for human recombination signal sequence-binidng protein (RBP-Jk) and corresponding processed psudogenes have been isolated from various species, such as Drosophila, Xenopus, mouse, and human. Here we report the isolation of another two genomic pseudogenes of human RBP-Jk, named K2 and K7, from a cosmid library of Hela cells. The nucleotide sequences of both genes exhibited more than 95 ~ homology to the functional human gene for RBP-Jk. Moreover, they did not contain any intron sequences and were interrupted by several stop codons in all frames. In situ hybridization demonstrated that the pseudogenes, K2 and K7, were localized at chromosomes 9p13 and 9q13, respectively. Their physical maps differed from those of the true functional gene and of the pseudogenes reported previously by Amakawa et al. (1993).

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A mathematically designed STS primer without any mismatches for direct sequencing of cosmid DNA clones

Cited by 2 publications

References 17 publications

Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescencein situ hybridization

Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescencein situ hybridization

Genomic structure and chromosomal localization of processed pseudogenes for human RBP-Jk

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