A mechanism of growth inhibition by abscisic acid in germinating seeds of Arabidopsis thaliana based on inhibition of plasma membrane H+-ATPase and decreased cytosolic pH, K+, and anions

Pedra, Montserrat Planes; Niñoles, Regina; Rubio, Lourdes; Bissoli, Gaetano; Bueso, Eduardo; García‐Sánchez, María Jesús; Alejandro, Santiago; González-Guzmán, Miguel; Hedrich, Rainer; Rodriguez, Pedro L.; Fernández, José Antonio; Serrano, Ramón

doi:10.1093/jxb/eru442

Cited by 82 publications

(87 citation statements)

References 75 publications

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“…6 C and F). In a "linear" pathway, ABA binds to its receptors to inhibit PP2C-A, releasing SnRK2 into active forms that can directly inhibit AHA2-mediated acidification and thereby repress root growth (4,28,29). The peptide hormone RALF, in contrast, binds and activates receptor FER to directly inhibit AHA2-mediated root growth (12).…”

Section: Discussionmentioning

confidence: 99%

“…When ABA levels in plants increase, it will induce the formation of a protein complex consisting of PYR/PYL/RCAR receptor proteins interacting with A-type PP2Cs, and thus inhibit the phosphatase activity of PP2Cs and release the SnRK2 kinases in their active forms to trigger downstream events (1)(2)(3). These downstream processes include stomatal closure, activation of ABA response genes, and cell growth inhibition through repression of AHA2-mediated acidification of extracellular medium (1)(2)(3)(4). In all these signaling processes, the A-type PP2Cs serve as coreceptors of ABA, and their activity represents an on-off switch in control of ABA responses.…”

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confidence: 99%

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FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis

Chen

Liu

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Receptor-like kinase FERONIA (FER) plays a crucial role in plant response to small molecule hormones [e.g., auxin and abscisic acid (ABA)] and peptide signals [e.g., rapid alkalinization factor (RALF)]. It remains unknown how FER integrates these different signaling events in the control of cell growth and stress responses. Under stress conditions, increased levels of ABA will inhibit cell elongation in the roots. In our previous work, we have shown that FER, through activation of the guanine nucleotide exchange factor 1 (GEF1)/4/10-Rho of Plant 11 (ROP11) pathway, enhances the activity of the phosphatase ABA Insensitive 2 (ABI2), a negative regulator of ABA signaling, thereby inhibiting ABA response. In this study, we found that both RALF and ABA activated FER by increasing the phosphorylation level of FER. The FER loss-of-function mutant displayed strong hypersensitivity to both ABA and abiotic stresses such as salt and cold conditions, indicating that FER plays a key role in ABA and stress responses. We further showed that ABI2 directly interacted with and dephosphorylated FER, leading to inhibition of FER activity. Several other ABI2-like phosphatases also function in this pathway, and ABA-dependent FER activation required PYRABACTIN RESISTANCE (PYR)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR)-A-type protein phosphatase type 2C (PP2CA) modules. Furthermore, suppression of RALF1 gene expression, similar to disruption of the FER gene, rendered plants hypersensitive to ABA. These results formulated a mechanism for ABA activation of FER and for cross-talk between ABA and peptide hormone RALF in the control of plant growth and responses to stress signals.signal transduction | root growth | plant hormones

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis

Chen

Liu

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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217

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“…Pro35S:HAB1 (Saez et al, 2004) Pro35S:HAB1/brm-1 (Han et al, 2012) pp2ca-1 (SALK_028132) (Kuhn et al, 2006) 152 abi4 (SALK_080095) (Finkelstein, 1994) Pro35S:3HA-PP2CA Pro35S:3HA-SnRK2.2 (Planes et al, 2015) …”

Section: Discussionmentioning

confidence: 99%

“…These targets are usually integral-membrane proteins like ion channels and H + ATPases or enzymes associated such as NADPH oxidases. As a result, the ion and water fluxes are regulated for a fast ABA response and secondary messengers of stress like ROS, NO,Ca 2+ are produced (Kwak et al, 2003, Mori et al, 2006, Planes et al, 2015.…”

Section: Core Signaling Pathwaymentioning

confidence: 99%

“…NADPH oxidases are also phosphorylated by SnRK2.6 to activate reactive oxygen species (ROS) production in guard cells (Sirichandra et al, 2009). Also, SnRKs can phosphorylate their targets to inactivate them as is the case of the plasma membrane ATPase and the potassium channel, KAT1 (Planes et al, 2015, Sato et al, 2009). All these targets are responsible of keeping the cellular homeostasis controlled in order to close the stomata and avoid water loss.…”

Section: Snf1-related Protein Kinases2 Snrk2smentioning

confidence: 99%

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Fundamental and applied research in ABA signaling: Regulation by ABA of the chromatin remodeling ATPase BRAHMA and biotechnological use of the PP2CA promoter.

Peirats‐Llobet¹

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Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic and dynamic chromatin state changes have been implicated in the plant response to the drought stress hormone abscisic acid (ABA). The Arabidopsis SWI/SNF chromatin-remodeling ATPase BRAHMA (BRM) modulates response to ABA by preventing premature activation of stress response pathways during germination. Here, we show that the core ABA signalosome formed by ABA receptors, PP2Csand SnRK2s physically interact with BRM to regulate BRM activity and post-translationally modify BRM by phosphorylation/dephosphorylation.Genetic evidence suggests that BRM acts downstream of SnRK2.2/2.3 kinases and biochemical studies identified evolutionary conserved SnRK2 phosphorylation sites in the C-terminal region of BRM. Our data suggest that SnRK2-dependent phosphorylation of BRM leads to its inhibition, and PP2CA-mediated dephosphorylation of BRM restores the ability of BRM to repress ABA response.ABA plays a key role to regulate germination and post-germination growth and the AP2-type ABI4 and bZIP-type ABI5 transcription factors RESUMENLa respuesta óptima a la sequía es crítica para la supervivencia de las plantas y afectará a la biodiversidad y al rendimiento de los cultivos durante el cambio climático. Las modificaciones epigenéticas y los cambios dinámicos del estado de la cromatina han sido implicados en la respuesta de la planta al ácido abscísico (ABA), la conocida como la hormona del estrés hídrico. La ATPasa remodeladora de cromatina de tipo SWI/SNF de Arabidopsis, BRAHMA (BRM), modula la respuesta al ABA mediante la prevención de la activación prematura de las vías de respuesta al estrés durante la germinación. Aquí, mostramos que el núcleo del señalosoma de ABA formado por los receptores de ABA, las PP2Cs y las SnRK2s interaccionan físicamente con BRM para regular su actividad y modificarla post-traduccionalmente por mecanismos de fosforilación/desfosforilación. La evidencia genética sugiere que BRM actúa aguas abajo de las quinasas SnRK2.2/2.3 y los estudios bioquímicos identificaron la presencia en la región C-terminal de BRM de sitios de fosforilación de las SnRK2 que estaban conservados evolutivamente. Nuestros datos sugieren que la fosforilación de BRM que depende de las SnRK2 conduce a su inhibición, y que la desfosforilación de BRM mediada por PP2CA restaura la capacidad de BRM para reprimir la respuesta a ABA.El ABA juega un papel clave en la regulación de la germinación y el crecimiento post germinativo y los factores de transcripción de tipo AP2 como ABI4 y de tipo bZIP como ABI5, son necesarios para la inhibición del crecimiento post germinativo mediado por ABA cuando los embriones encuentran estrés hídrico. La detención del crecimiento inducida por ABI4 y ABI5 implica la señalización de ABA y en el caso de ABI5, se ha demostrado que el ABA inhibe la actividad de BRM para ! inducir la transcripción de ABI5. La pérdida de actividad de B...

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Auxin as a Mediator of Abiotic Stress Responses

Salopek‐Sondi

Pavlović

Smolko

et al. 2017

Mechanism of Plant Hormone Signaling Under Stress

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A mechanism of growth inhibition by abscisic acid in germinating seeds of Arabidopsis thaliana based on inhibition of plasma membrane H+-ATPase and decreased cytosolic pH, K+, and anions

Abstract: HghlightWe provide the first evidence for a mechanism of growth inhibition by ABA during germination and seedling establishment based on inhibition of PM H+-ATPase and altered pH, K+, and anion homeostasis.

Cited by 82 publications

References 75 publications

FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis

FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis

Fundamental and applied research in ABA signaling: Regulation by ABA of the chromatin remodeling ATPase BRAHMA and biotechnological use of the PP2CA promoter.

Auxin as a Mediator of Abiotic Stress Responses

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