A mechanistic approach for in‐vitro anticancer activity via nucleic acid fragmentation by copper(II) complex anchored on MCM‐41

Abstract

Three new mononuclear copper Schiff base complexes, namely, ([Cu(L1)Cl].CH3CN (HmC_1), [Cu(L2)Cl].CH3CN (HmC_2) and [Cu(L3)Cl].CH3CN (HmC_3) derived from ONO donor tridentate ligands HL1, HL2 and HL3, respectively, have been synthesized to check their efficacy as target‐specific next‐generation anticancer therapeutics. All the HmCs have been characterized by using various physicochemical techniques (i.e., single‐crystal X‐ray analysis, Fourier transform infrared [FT‐IR] spectroscopy and elemental analysis). Among the synthesized Schiff base complexes, HmC_3 was turned out to be most effective in killing cancer cell carried out on cultured human breast cancer cell line (MDA‐MB‐231) and human lung carcinoma cell line (A549). Finally, in order to improve the cellular permeability, particle size of HmC_3 was scaling down to nano‐regime by immobilizing it onto a suitable matrix MCM‐41@APTES (MCM‐41 = Mobil Composition of Matter No. 41 and APTES = 3‐aminopropyltriethoxysilane) to generate MCM‐41@APTES@HmC_3 (MCM‐41@APTES@HmC_3 = HtC_3). Field‐emission scanning electron microscope (FESEM) and dynamic light scattering (DLS) study revealed the particle size of the synthesized HtC_3 nano‐composite was within nano‐regime and therefore could be further effective for biomedical applications. Furthermore, HtC_3 displayed better cancer cell killing property. To get insight into the mechanism of action of HtC_3, Annexin V‐FITC/PI analysis and TUNEL assay revealed that DNA damage phenomenon is accompanied with changes in cellular morphology leading to cell apoptosis. Cell migration assay on MDA‐MB‐231 cell with HtC_3 exposed the effectiveness of HtC_3 in arresting cancer cell migration. Therefore, all the in‐vitro studies demonstrated that the immobilization of the copper Schiff base complex onto a suitable matrix increased its efficiency and becomes a promising anticancer nano‐therapeutic agent.

Decision-Making Software-Integrated Ultrafast Detection of Lead in Surface Water Using a Chemo-Nano Sensing Device

“…The band near 1410 cm –1 is observed due to the benzene skeletal vibration . The characteristic peaks of the additional chemical moieties were comparable to the previously reported results . The data confirm the anchoring of the homogeneous CuCC complexes into the heterogeneous matrix of MCM, forming CuCC@MCM.…”

“…MCM was selected as a heterogeneous backbone that can provide a large surface area, high pore volume, and tunable nanoscale pores. It was also synthesized in-house by following the protocol reported by Karim et al, 2022 with modification . Briefly, in an acidic aqueous solution of TA (0.85 g in 100 mL of H 2 O), 4.48 g of TEOS was dissolved under a vigorous stirring condition.…”

“…It was also synthesized in-house by following the protocol reported by Karim et al, 2022 with modification. 35 Briefly, in an acidic aqueous solution of TA (0.85 g in 100 mL of H 2 O), 4.48 g of TEOS was dissolved under a vigorous stirring condition. After half an hour, 3.5 g of cationic CTAB and nonionic polyethylene glycol dodecyl ether were added to this mixture.…”

“…37 The characteristic peaks of the additional chemical moieties were comparable to the previously reported results. 35 The data confirm the anchoring of the homogeneous CuCC complexes into the heterogeneous matrix of MCM, forming CuCC@MCM. Further, the change in the total composition of the synthesized complex after coating on the electrode surface was then validated.…”

Synthesis and catalytic applications of organo‐functionalized MCM‐41 catalyst: A review

“…Among the materials synthesized, M48 was most potent in killing cancer cells in cultured cancer cell lines (MDA‐MB‐231) and carcinoma cell lines (A549) of human breast and lung, respectively. [ 98 ]…”