A mediated turnip tissue paper-based amperometric hydrogen peroxide biosensor

Sekar, N.; Ge, Liya; Shaegh, Seyed Ali Mousavi; Ng, Sum Huan; Tan, Swee Ngin

doi:10.1016/j.snb.2014.12.045

Cited by 30 publications

(26 citation statements)

References 38 publications

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“…Once dry, the discs were placed on a DropSens electrode. The integration of the protein laden disc and SPCE has been described in detail elsewhere [37]. For the Hg 2+ experiments measurements were taken with an incubation time of 2 min to allow for equilibration.…”

Section: Methodsmentioning

confidence: 99%

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

2017

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Metallothioneins (MTs) are a family of cysteine-rich proteins whose biological roles include the regulation of essential metal ions and protection against the harmful effects of toxic metals. Due to its high affinity for many toxic, soft metals, recombinant human MT isoform 1a was incorporated into an electrochemical-based biosensor for the detection of As3+ and Hg2+. A simple design was chosen to maximize its potential in environmental monitoring and MT was physically adsorbed onto paper discs placed on screen-printed carbon electrodes (SPCEs). This system was tested with concentrations of arsenic and mercury typical of contaminated water sources ranging from 5 to 1000 ppb. The analytical performance of the MT-adsorbed paper discs on SPCEs demonstrated a greater than three-fold signal enhancement and a lower detection limit compared to blank SPCEs, 13 ppb for As3+ and 45 ppb for Hg2+. While not being as low as some of the recommended drinking water limits, the sensitivity of the simple MT-biosensor would be potentially useful in monitoring of areas of concern with a known contamination problem. This paper describes the ability of the metal binding protein metallothionein to enhance the effectiveness of a simple, low-cost electrochemical sensor.

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Section: Methodsmentioning

confidence: 99%

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

2017

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“…Amperometric sensors have experienced a lot of improvement over the past five years, including improvements to stability [64,65], detection limit [65], and cost [66]. Since H 2 O 2 can decay in rapid and unpredictable ways, Draminska et al developed a bienzymatic system to detect the catalase reaction as well as the decay of the H 2 O 2 [67]; their bienzymatic sensor is created by coating a glassy carbon electrode in multi-walled carbon nanotubes with absorbed catalase and either laccase or bilirubin oxidase [67].…”

Section: Developments From 2015 To 2019mentioning

confidence: 99%

“…Many amperometric sensors use enzymes, but this unfortunately leads to increased costs due to the extraction and purification techniques that are necessary to acquire the enzymes [69,70]. In 2015, Sekar et al circumvented this extraction and purification issue by immobilizing raw turnip peroxidase and potassium hexacyanoferrate into a cellulose paper that is both disposable and biodegradable [66]. Sekar et al's sensor, which can be used to detect H 2 O 2 in a commercial wound disinfectant, does use an enzyme, but is able to do so in a more cost-effective manner [66].…”

Section: Enzyme-free Sensorsmentioning

confidence: 99%

Hydrogen Peroxide Sensors for Biomedical Applications

et al. 2019

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Hydrogen peroxide (H2O2) is an important molecule within the human body, but many of its roles in physiology and pathophysiology are not well understood. To better understand the importance of H2O2 in biological systems, it is essential that researchers are able to quantify this reactive species in various settings, including in vitro, ex vivo and in vivo systems. This review covers a broad range of H2O2 sensors that have been used in biological systems, highlighting advancements that have taken place since 2015.

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“…Then, 7 μL of the potato extract was carefully spotted onto the center of each paper sheet. After being dried, these modified paper sheets were stored in a refrigerator (4°C) for the next experiments [33].…”

Section: Preparation Of the Paper Based Nanobiosensormentioning

confidence: 99%

Rapid Determination of Phenolic Compounds in Water Samples: Development of a Paper‐based Nanobiosensor Modified with Functionalized Silica Nanoparticles and Potato Tissue

et al. 2019

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In this study, we present a fast, simple, low‐cost and disposable method for determination of phenolic content in water samples, using a paper based polyphenol oxidase biosensor. The propylamine functionalized silica nanoparticles was dropped onto a paper sheet. After drying at room temperature, the potato tissue extract including polyphenol oxidase was immobilized on the paper via physical and chemical adsorption. The modified paper was placed on the top of the graphite screen printed electrode. To construct of an electrochemical nanobiosensor, the electrochemical behavior of the modified electrode in different steps was investigated by cyclic voltammetry and electrochemical impedance spectroscopy methods. After being optimized the effective parameters, the changes in the biosensor electrochemical response vs. to the different concentrations of the substrate (phenol solution) were monitored by differential pulse voltammetry and amperometry methods. The linear relationships for phenol detection were obtained in the concentration ranges of 0.01–160 μM and 0.1–300 μM with a detection limit of 0.007 μM and 0.042 μM with DPV and amperometry methods, respectively. This method was successfully used in the voltammetric determination of the phenol content in the real samples, like the river water and the wastewater of wood factory.

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A mediated turnip tissue paper-based amperometric hydrogen peroxide biosensor

Cited by 30 publications

References 38 publications

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

Hydrogen Peroxide Sensors for Biomedical Applications

Rapid Determination of Phenolic Compounds in Water Samples: Development of a Paper‐based Nanobiosensor Modified with Functionalized Silica Nanoparticles and Potato Tissue

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