A metabolomics approach to study the dual modulation by characterization of chemical alteration during processing of Gardeniae Fructus using UPLC-ESI-QTOF

Li, Huan; Hong, Yang; Xue, Xuejia; Liu, Xiao; Tian, Fajun; Poh, Yanhong; Cai, Hao; Lee, Yian Hoon; Yu, Hanry; Ong, Seng Poon; Cai, Baochang

doi:10.1039/c5ay03265b

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…Currently, some efforts have been made to study the changes in the processing of GFP. A strategy by UPLC-ESI-QTOF and a multivariate statistical analysis found that some chemical changes existed between GF and GFP, showing downward trends of geniposide, genipin-1- O -gentiobioside, 6a-hydroxygeniposide, jasminoside B, crocin, and mannitol, while mussaenosidic acid increased [ 12 ]. The study with the aid of a UPLC characteristic chromatogram and color value demonstrated that there were significant differences in crocins as well as chroma values between GFP and other processed products [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Quality Evaluation of Gardeniae fructus Praeparatus during Heat Processing Based on Sensory Characteristics and Chemical Compositions

et al. 2022

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The intrinsic chemical components and sensory characteristics of Gardeniae fructus Praeparatus (GFP) directly reflect its quality and subsequently, affect its clinical curative effect. However, there is little research on the correlation between the appearance traits and chemical compositions of GFP during heat processing. In this study, the major components of five typical processed decoction pieces of GFP were determined. With the deepening of processing, the contents of geniposidic acid and 5-HMF gradually increased, while the contents of deacetyl-asperulosidic acid methyl ester, gardenoside, and two pigments declined. Moreover, the electronic eye, electronic tongue, and electronic nose were applied to quantify GFP’s sensory properties. It was found that the chroma values showed a downward trend during the processing of GFP. The results of odor showed that ammonia, alkenes, hydrogen, and aromatic compounds were the material base for aroma characteristics. Complex bitterness in GF was more obvious than that in other GFP processed products. Furthermore, one mathematical model was established to evaluate the correlation between the sensory characteristics and chemical composition of GFP during five different stages. A cluster analysis and neural network analysis contributed to recognizing the processing stage of GFP. This study provided an alternative method for the exterior and interior correlation-based quality evaluation of herbs.

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Section: Introductionmentioning

confidence: 99%

A Novel Method for Quality Evaluation of Gardeniae fructus Praeparatus during Heat Processing Based on Sensory Characteristics and Chemical Compositions

et al. 2022

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“…Some researchers argue that the changes in chemical constituents of GFP associated with hemostasis are vital for stopping the bleeding. For example, the contents of iridoid glycoside and crocin with antithrombotic effects are lower in GFP compared to GF ( 19 , 20 ), while the effective hemostatic components, such as tannin and crocetin, are higher in GFP than in GF ( 21 , 22 ), which contributes to the hemostatic effect of GFP. These pharmacodynamic experiments have demonstrated initial success in the procoagulant activity of GFP but have not examined the mechanism of action.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the hemostatic mechanism of Gardeniae fructus Praeparatus based on pharmacological evaluation and network pharmacology

Zheng¹,

Wang²,

Xia³

et al. 2022

Ann Transl Med

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Background: As documented in the Chinese Pharmacopoeia, Gardeniae fructus Praeparatus (GFP) can cool the blood during hemostasis and treat various internal hemorrhagic diseases. However, the underlying mechanisms are not yet well understood. This work was designed to decipher the possible mechanism by which GFP prevents hemorrhage. The integration of pharmacodynamics-based and bioinformatics-based methods provided evidence to support the clinical effects of GFP in treating bleeding.Methods: Using ultra-performance liquid chromatography (UPLC) analysis, we quantified the main active ingredients for a preliminary quality assessment of GFP. The pharmacology study was conducted to confirm the essential antihemorrhagic effects of GFP. A rat model of ethanol-induced gastric hemorrhage was established and was followed by intervention with GFP in low, middle, and high doses (4.5, 9, 18 g/kg).Gastric tissues were harvested for macroscopic and histological evaluation of lesions. The contents of thromboxane B2 (TXB 2 ) and 6-keto-prostaglandin-F1α (6-keto-PGF 1α ) in the serum were determined.Additionally, network pharmacology was proposed to illuminate the potential mechanisms. Following the collection of GFP compositions, the compound-and hemorrhage-related targets were retrieved from public databases. The protein-protein interaction (PPI), gene ontology, pathways analysis, and molecular docking were performed for targets of GFP in gastrointestinal bleeding. Results:The study found ten main active ingredients that could be used for quality control of GFP.Importantly, the middle and high doses of GFP were found to promote the healing of gastric bleeding.The content of 6-keto-PGF 1α was significantly degraded in the middle and high treated groups (P<0.05).The level of TXB 2 was augmented by a middle (P<0.05) and high dose of GFP. Further, we constructed the network of candidate ingredients and hemorrhage-related targets. Pathway analysis predicted the mechanisms associated with interleukin 4 and interleukin 13 signaling and platelet activation. PPI analysis identified subnetworks with biological functions and also sifted hub targets that affected the antihemorrhagic progress. The candidate proteins had a good binding force with major components.Conclusions: GFP exhibits a promising effect in ameliorating bleeding, with the relevant molecular mechanisms possibly being related to the regulation of the immune system and platelet activation. Therefore, GFP can potentially exert a protective effect on gastrointestinal bleeding in clinic.

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Discovery of minor quality evaluation marker compounds for Chinese patent medicine products using a two-leveled metabolomics strategy

Xue

Shang

et al. 2021

Journal of Chromatography A

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A metabolomics approach to study the dual modulation by characterization of chemical alteration during processing of Gardeniae Fructus using UPLC-ESI-QTOF

Cited by 5 publications

References 29 publications

A Novel Method for Quality Evaluation of Gardeniae fructus Praeparatus during Heat Processing Based on Sensory Characteristics and Chemical Compositions

A Novel Method for Quality Evaluation of Gardeniae fructus Praeparatus during Heat Processing Based on Sensory Characteristics and Chemical Compositions

Investigation of the hemostatic mechanism of Gardeniae fructus Praeparatus based on pharmacological evaluation and network pharmacology

Discovery of minor quality evaluation marker compounds for Chinese patent medicine products using a two-leveled metabolomics strategy

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