A metallothionein-like protein of rice (rgMT) functions in E. coli and its gene expression is induced by abiotic stresses

Jin, Shumei; Cheng, Yuxiang; Guan, Qing; Liu, Dali; Takano, Tetsuo

doi:10.1007/s10529-006-9152-1

Cited by 58 publications

(35 citation statements)

References 14 publications

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“…E. coli cells expressing pET-30a-EhMT1 were more tolerant to Cu exposure and accumulated more Cu than control cells. Similar results were reported in E. coli expressing PsMT A and rgMT (Evans et al 1992;Jin et al 2006). AtMT1 from Arabidopsis enhances Cu resistance in yeast cells (Zhou and Goldsbrough 1994).…”

Section: Discussionsupporting

confidence: 84%

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Xia

Yuan

et al. 2012

Acta Physiol Plant

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EhMT1, a type 1 metallothionein (MT) gene, was cloned from Elsholtzia haichowensis, a Cu-tolerant plant. Typical of plant type 1 MTs, EhMT1 encodes a putative peptide of 74 amino acid residues containing cysteine-rich domains. Sequence comparisons with various databases revealed strong similarities at the nucleotide and amino acid levels between EhMT1 and the type 1 MT of Mimulus guttatus. EhMT1 transcription was greater in the roots than in the leaves, and was markedly increased by treatments with Cu, H 2 O 2 , and heat shock. EhMT1-GUS was localized to the cytoplasm of onion epidermal cells. Escherichia coli cells expressing pET-30a-EhMT1 were more tolerant to Cu and accumulated more Cu than control cells. Our results show that EhMT1 is involved in Cu tolerance and accumulation in E. haichowensis.

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Section: Discussionsupporting

confidence: 84%

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Xia

Yuan

et al. 2012

Acta Physiol Plant

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“…Rice OsMT expression was induced by several abiotic stresses including salts (NaCl and NaHCO 3 ), drought (PEG), and metal ions (CuCl 2 , ZnCl 2 , and CdCl 2 ) [28]. In the current study, IbMT1 and IbMT3 exhibited similar expression patterns in response to abiotic stresses and heavy metals (Figs.…”

Section: Discussionsupporting

confidence: 64%

Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals

et al. 2014

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Metallothioneins (MTs) are cysteine-rich, low molecular weight, metal-binding proteins that are widely distributed in living organisms. Plants produce metal-chelating proteins such as MTs to overcome the toxic effects of heavy metals. We cloned three MT genes from sweetpotato leaves [Ipomoea batatas (L.) Lam.]. The three IbMT genes were classified according to their cysteine residue alignment into type 1 (IbMT1), type 2 (IbMT2), and type 3 (IbMT3). IbMT1 was the most abundantly transcribed MT. It was predominantly expressed in leaves, roots, and callus. IbMT2 transcript was detected only in stems and fibrous roots, whereas IbMT3 was strongly expressed in leaves and stems. The IbMT expression profiles were investigated in plants exposed to heavy metals and abiotic stresses. The levels of IbMT1 expression were strongly elevated in response to Cd and Fe, and moderately higher in response to Cu. The IbMT3 expression pattern in response to heavy metals was similar to that of IbMT1. Exposure to abiotic stresses such as methyl viologen (MV; paraquat), NaCl, polyethylene glycol (PEG), and H2O2 up-regulated IbMT expression; IbMT1 responded strongly to MV and NaCl, whereas IbMT3 was induced by low temperature and PEG. Transgenic Escherichia coli overexpressing IbMT1 protein exhibited results suggest that IbMT could be a useful tool for engineering plants with enhanced tolerance to environmental stresses and heavy metals.

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“…Metallothionein is a well-known chelator of Cd/Cu both in yeast and in rice (Ecker et al, 1986;Jin et al, 2006). Expression of Ta HsfA4a induced CUP1 expression in yeast and Os MT-I-1a expression in rice ( Figures 5A and 6C), while deletion of the metallothionein gene abolished the Ta HsfA4a-mediated Cd tolerance in yeast ( Figure 5B).…”

Section: Discussionmentioning

confidence: 95%

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

et al. 2009

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Cadmium (Cd) is a widespread soil pollutant; thus, the underlying molecular controls of plant Cd tolerance are of substantial interest. A screen for wheat (Triticum aestivum) genes that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a (HsfA4a). Ta HsfA4a is most similar to the class A4 Hsfs from monocots. The most closely related rice (Oryza sativa) homolog, Os HsfA4a, conferred Cd tolerance in yeast, as did Ta HsfA4a, but the second most closely related rice homolog, Os HsfA4d, did not. Cd tolerance was enhanced in rice plants expressing Ta HsfA4a and decreased in rice plants with knocked-down expression of Os HsfA4a. An analysis of the functional domain using chimeric proteins constructed from Ta HsfA4a and Os HsfA4d revealed that the DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Moreover, Ta HsfA4a-mediated Cd resistance in yeast requires metallothionein (MT). In the roots of wheat and rice, Cd stress caused increases in HsfA4a expression, together the MT genes. Our findings thus suggest that HsfA4a of wheat and rice confers Cd tolerance by upregulating MT gene expression in planta.

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A metallothionein-like protein of rice (rgMT) functions in E. coli and its gene expression is induced by abiotic stresses

Cited by 58 publications

References 14 publications

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

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