A Method for in vitro Studies on Fluoride Uptake in Enamel Using Single Tooth Surfaces

Self Cite

The fluoride uptake from six different fluoride solutions applied to the buccal surfaces of extracted, newly erupted premolars was measured. The fluoride concentration of two symmetrically situated areas were compared at various distances from the enamel surface after treatment of one of the areas with a fluoride solution. Exposure of enamel to sodium fluoride, acidic phosphate fluoride, monofluorophosphate, stannous fluoride, sodium hexafluorostannate and cetylaminhydrofluoride was found to produce significant but widely variable increases in the fluoride content of the superficial layers. The uptake of fluoride from all test solutions, except cetylaminhydrofluoride appeared to be confined mainly to the outermost 10–15 μm of enamel.

Section: Methodsmentioning

confidence: 99%

In vitro Fluoride Uptake in Human Dental Enamel from Various Fluoride Solutions

Kirkegaard¹

1977

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“…On the other hand, a 30-40% reduction in caries increment has been shown after a few topical applications of a concentrated fluo ride solution [Horowitz, 1973], The moderate clinical effect of fluoride dentifrices, may be related to the insignifi cant penetration of F into the enamel as demonstrated in the present study. Topical application of fluoride results in much high er concentrations of fluoride in the enamel when measured by a similar technique [Kirkegaard, 1976]. Fluoride-containing denti frice may thus exert this effect by mecha nisms other than reduction of enamel solu bility through fluoride incorporation in the enamel.…”

Section: Discussionmentioning

confidence: 99%

“…Fluoride uptake studies using enamel powder have proven valuable in the evaluation of dentifrices. However, the fluoride uptake of intact enamel may not be the same as that of enamel powder [Ericsson, 1961], The purpose of the pre sent study was to evaluate the fluoride up take into intact enamel surfaces from four fluoride-containing dentifrices using a standardized in vitro method described pre viously [Kirkegaard et al, 1976].…”

mentioning

confidence: 99%

In vitro Fluoride Uptake in Human Dental Enamel from Four Different Dentifrices

Kirkegaard¹

1977

Self Cite

The in vitro fluoride uptake in human dental enamel treated with four different dentifrices was evaluated using a standardized technique. The fluoride concentration in two symmetrical areas of buccal surfaces of newly erupted premolars was analyzed in eight subsequent layers. One of the areas was treated with the dentifrice and the other served as a control. Fluoride gradient curves were computed for the two areas and the differences in the fluoride concentration at various distances from the enamel surface was calculated. Application of a dentifrice resulted in a small increase in the fluoride concentration of the enamel surface. After 1 h of treatment, however, the fluoride uptake was confined to the outer 5 μm of the enamel surface. No definite relationship was found between the fluoride ion activities in the dentifrice slurries applied to the tooth surface and the fluoride uptake. The small fluoride uptake in the enamel from the dentifrices, containing fluoride, may possibly be one reason for the moderate caries reduction obtained by daily use of fluoride-containing dentifrices.

“…Although fluoride concen tration in the enamel surface is variable, the average fluoride concentrations of two symmetrical areas on a tooth surface are almost identical [Brunn, 1973;Kirkegaard et al, 1976;Retief et al, 1980], making them suitable to compare pre-and post-treatment flu oride levels. In this investigation, samples from two such areas at each site were used to calculate uptake.…”

Section: Pre-treatment Fluoride Concentrationsmentioning

confidence: 99%

Enamel Fluoride Uptake Affected by Site of Application: Comparing Sodium and Amine Fluorides

Mok¹,

Hill²,

Newman

1990

In enamel fluoride uptake studies, the most frequently sampled site is the middle third of the buccal surface. Because different parts of the enamel surface vary in fluoride concentration, the present study investigated fluoride uptake at contrasting sites using two different topical agents. One was a neutral aqueous solution of sodium fluoride containing 2% w/w of fluoride, and the second was an aqueous solution of two amine fluorides containing 1% w/w of fluoride. The enamel of 10 pairs of clinically sound extracted human premolars was etched initially and after treatment with one of these agents on the cervical and middle thirds of the buccal surface and on the proximal surface, yielding the pre- and post-treatment fluoride concentrations of these sites at depths of 5 and 10 μm from the surface. Enamel treated with the amine F solution had significantly higher fluoride uptakes at all sites compared to the NaF-treated specimens. The differences in uptake from the two agents varied with site, being smallest for the buccal middle third enamel and greatest for the proximal enamel. It is suggested that these results relate to possibje differences in enamel maturation or to the presence of initial proximal caries, and the greater affinity of amine fluoride for porous enamel. The findings emphasise the importance of obtaining site-specific data in the study of fluoride in enamel.