A Method for Local Labelling of the Thymus and of the Bursa of Fabricius of the Chicken With Tritiated Thymidine

Linna, T. Juhani; Hemmingsson, E. J.; Bäck, Rune

doi:10.1111/j.1699-0463.1969.tb04262.x

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous report found that the peripheralization of bursacytes in blood and the spleen starts at the 18 th day of embryogenesis in chickens using a 3 H-thymidine assay (Woods & Linna 1965;Linna et al 1969). The flow cytometric data of the present study indicated the presence of a small number of sIgM + cells in the spleen at e18, but there was no difference in the sIgM + cell rate between splenocytes of e18 and those of e12.…”

Section: Discussionsupporting

confidence: 44%

“…A previous report found that the peripheralization of bursacytes in blood and the spleen starts at the 18 th day of embryogenesis in chickens using a 3 H‐thymidine assay (Woods & Linna 1965; Linna et al. 1969).…”

Section: Discussionmentioning

confidence: 99%

“…Matured B cells distribute throughout the body from the bursa of Fabricius via the blood stream, and colonize peripheral lymphoid organs such as the spleen and cecal tonsil from e18 day in chick embryos (Woods & Linna 1965;Linna et al 1969). In the peripheral organs, antigen stimulation gives rise to B cell differentiation into antibody-producing cells via clonal expansion.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

Narabara

Abe

Gerilechaogetu

et al. 2009

Animal Science Journal

View full text Add to dashboard Cite

Flow cytometric analysis and immunohistochemical observation were used to qualitatively and quantitatively clarify the nature of B cell differentiation in the bursa of Fabricius of chick embryos and to determine the timing of antibody class switching in chicken spleens based on positivity of IgM and IgG on and in the cells. In the bursa, the sIgM-positive cell population formed from the 12(th) to 15(th) day of embryogenesis. The proportion of sIgM-high expressing (sIgM(high)) cells was lower among bursacytes than splenocytes of hatched chicks, suggesting that the sIgM(high) bursacytes are to be released to peripheral sites. The proportion of sIgM(high) cells was higher at 0 days old than at any other examined stage of development. Colonization of the spleen by B cells occurred between the 18(th) day of embryogenesis and 0 days old. Antibody class switching was thought to start in the spleen between 1 and 2 weeks of age, because IgG-positive cells were present in the spleen of 2-week-old chicks, but not 0-day-old or 1-week-old chicks.

show abstract

Section: Discussionsupporting

confidence: 44%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

Narabara

Abe

Gerilechaogetu

et al. 2009

Animal Science Journal

View full text Add to dashboard Cite

show abstract

Influence of antigenic stimulation on lymphoid cell traffic in the chicken

Bäck

Linna

1973

Eur J Immunol

Self Cite

View full text Add to dashboard Cite

These individual probabilities are given by the hypergeometric distribution and the result reduces to:where C is independent of N, and bl+bol+bool+bml = total number (D) of different antibody molecules detected in the whole sample.From this result, relative probabilities for different values of N can be calculated using Stirling's approximation for factorial For hand calculation, the value of N giving the greatest probability (i.e. the maximum likelihood estimate of N) can most easily be found by considering the ratio of these probabilities for successive values of N. The estimate is then the largest valur of N for which Probabilities corresponding t o the data of Table 4 (i.e. n l =138, n,=6l, n3=67, n4=71, D=332) are shown in Fig. 4. If all assumptions are correct, a value of approximately 8000 can be given as an estimate of N, although clearly there is even then a wide range of likely values.Within a particular donor/recipients system some antibody types may have a greater chance of being sampled than others. However assumption (ii) is invalid only if this difference depends on some property of the antibody types (i.e. if some types have a relatively high probability of selection in all donor/recipients systems). If assumption (ii) is invalid, or if some of the spectra that were considered identical were in fact produced by different antibodies, the above method might be expected t o underestimate N. We might then consider 2000 to represent the smallest likely value, but no upper limit could then be given.However, let us now consider the case in which each mouse is genetically capable of producing only A (less than N) different antibody types (i.e. assumption (i) is invalid), and the A different types are not necessarily the same for all four mice. If we accept assumption (ii), the method given above should in this case be regarded as providing an overestimate of A rather than an estimate of N. If A is only slightly less than N, the degree by which A is overestimated should be small and 2000 as a minimum estimate of both A and N is unlikely t o be misleading. If A is much less than N, then, in general, no meaning can be attached t o the estimate. However, in this particular case, estimates of the number of different antibodies actually produced by a donor mouse (based on an analysis of the frequency of occurrence of identical antibodies in different mice receiving cell transfer from the same donor mouse) suggest that 2000, as a minimum estimate of N, is still unlikely t o be misleading.

show abstract

DIFFERENCES IN ³H‐THYMIDINE UPTAKE BY CHICKEN LYMPHOID TISSUES AFTER INTRAVENOUS OR INTRAPERITONEAL ADMINISTRATION

Hemmingsson

1972

Acta Pathologica Microbiologica Scandinavica Section A Patholog

View full text Add to dashboard Cite

Intraperitoneal injection of tritiated thymidine, as compared to intravenous injection, was shown to cause a local labelling of the organs inside the peritoneal cavity. This labelling was most intensive in organs situated in the part of the cavity where the injection was made. In autoradiographs of ileum from the intraperitoneally labelled animals heavily labelled cells were found in the serous membrane, the muscular layers, and even in the lamina propria close to the surface epithelium. It could be calculated that the tritiated thymidine available in the general circulation after an intraperitoneal injection was about 60 per cent of that available after an intravenous injection of the same dose.

show abstract

A Method for Local Labelling of the Thymus and of the Bursa of Fabricius of the Chicken With Tritiated Thymidine

Cited by 3 publications

References 0 publications

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

Influence of antigenic stimulation on lymphoid cell traffic in the chicken

DIFFERENCES IN ³H‐THYMIDINE UPTAKE BY CHICKEN LYMPHOID TISSUES AFTER INTRAVENOUS OR INTRAPERITONEAL ADMINISTRATION

Contact Info

Product

Resources

About

A Method for Local Labelling of the Thymus and of the Bursa of Fabricius of the Chicken With Tritiated Thymidine

Cited by 3 publications

References 0 publications

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

Influence of antigenic stimulation on lymphoid cell traffic in the chicken

DIFFERENCES IN 3H‐THYMIDINE UPTAKE BY CHICKEN LYMPHOID TISSUES AFTER INTRAVENOUS OR INTRAPERITONEAL ADMINISTRATION

Contact Info

Product

Resources

About

DIFFERENCES IN ³H‐THYMIDINE UPTAKE BY CHICKEN LYMPHOID TISSUES AFTER INTRAVENOUS OR INTRAPERITONEAL ADMINISTRATION