A method for micropropagation of Cornus wilsoniana: An important biofuel plant

Li, Yongxin; Wang, Xiaoming; Chen, Jianjun; Cai, Neng; Zeng, Huijie; Qiao, Zhongquan; Wang, Xiangying

doi:10.1016/j.indcrop.2015.06.042

Cited by 15 publications

(15 citation statements)

References 27 publications

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“…The information available on acclimation of Cornus is limited. However, previous reports indicate that 60–100% of plants can acclimate to ex vitro conditions depending on the species (Kaveriappa et al 1997 ; Ishimaru et al 1998 ; Ďurkovič 2008 ; Ďurkovič and Bukovská 2009 ; Li et al 2015 ).…”

Section: Introductionmentioning

confidence: 98%

“…Premature death of explants can be encountered as well as vitrification, chlorosis of leaves, and little or no root induction (Ilczuk et al 2013 ). Several studies have been carried out to optimize conditions for the regeneration and multiplication of different Cornus species (Lu 1984 , 1985 ; Lattier et al 2014 ; Li et al 2015 ). Regeneration efficiency depends on the medium composition: macro- and micronutrients, type and concentration of growth regulators, and carbon source.…”

Section: Introductionmentioning

confidence: 99%

“…The most commonly used media for tissue culture of dogwood are woody plant medium (WPM; Lloyd and McCown 1980 ) for Cornus florida and Cornus mas (Kaveriappa et al 1997 ; Ďurkovič 2008 ) and MS (Murashige and Skoog 1962 ) for Cornus kousa (Ishimaru et al 1993 ), and C. alba (Zhang and Li 2005a , b , 2010 ). Other media are less frequently reported to be used: Linsmaier and Skoog ( 1965 ) (LS) for Cornus officinalis (Ishimaru et al 1998 ), Schenk and Hildebrandt ( 1972 ) (SH) for C. florida (Trigiano et al 1989 ), Driver and Kuniyuki ( 1984 ) (DKW) for Cornus wilsoniana (Li et al 2015 ), and broad-leaved tree medium (BW; Sato 1991 ) for C. kousa and Cornus capitata (Ishimaru et al 1998 ; Hadziabdic 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…Sucrose concentration is a decisive factor for shoot regeneration and growth (Gibson 2000 ). Several authors recommend 30 g L −1 sucrose as the optimal concentration to guarantee a high multiplication rate in C. nuttallii, C. florida , and C. wilsoniana (Edson et al 1994 ; Sharma et al 2005 ; Li et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Ilczuk

Jacygrad

2016

In Vitro Cell.Dev.Biol.-Plant

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Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars ‘Aurea’ and ‘Elegantissima’ were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L−1 BA, 0.1 mg L−1 NAA, and 20–30 g L−1 sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L−1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for ‘Aurea’ and 90% for ‘Elegantissima’. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in ‘Aurea’ and ‘Elegantissima’ plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Ilczuk

Jacygrad

2016

In Vitro Cell.Dev.Biol.-Plant

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“…Ni is an essential micronutrient for plants (Brown et al, 1987;Eskew et al, 1983;Hand and Reed, 2014;Ragsdale, 1998). The same DKW medium has been shown to improve in vitro shoot multiplication of Cornus wilsoniana (Li et al, 2015). It might be possible that Ni is important to the growth of Weigela and its absence in the other media might affect axillary shoot production.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Weigela florida ‘Tango’ through In Vitro Shoot Culture

Wang¹,

Li²,

Zeng³

et al. 2017

horts

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Weigela florida (Bunge) A. DC. is a popular flowering shrub adapted to a wide range of environmental conditions. Efficient methods for micropropagation of this species have not been well developed. The present study established a protocol for in vitro shoot culture of W. florida ‘Tango’ after a systematic evaluation of different culture media, cytokinins, and auxins on axillary shoot induction. Single-node stems were cultured on Driver and Kuniyuki Walnut (DKW) medium for initial production of axillary shoots. The shoots were used as explants and cultured on DKW medium supplemented with 8.88 μm 6-benzylaminopurine (BA) and 0.27 μm naphthaleneacetic acid (NAA), resulting in the production of more than six axillary shoots per explant. The axillary shoots could either be used as explants for additional shoot production or be cultured on ½ DKW medium supplemented with 0.25 μm indole-3-butyric acid (IBA) for rooting. Plantlets were transplanted into a substrate with 99% survival rate in a shaded greenhouse. This established method could be used for rapid propagation of W. florida to speed the introduction of new hybrids or cultivars for commercial production.

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Micropropagation and HPLC-DAD, UPLC MS/MS analysis of oenothein B and phenolic acids in shoot cultures and in regenerated plants of fireweed (Chamerion angustifolium (L.) Holub)

Dreger

Gryszczyńska

Szalata

et al. 2020

Plant Cell Tiss Organ Cult

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In this study, a micropropagation protocol using nodal explants from in vitro grown plants of Chamerion angustifolium (L.) Holub was developed and analysis of oenothein B and selected phenolic acids in shoot cultures was performed for the first time. For shoot induction and multiplication Murashige and Skoog’s (MS) basal medium supplemented with 2-isopentenyladenine (2iP), zeatin (Z) and 6-benzyloaminopurine (BAP) was used. 2iP was the most responsive in terms of promoting shoots per explant with the maximum (6.57 ± 1.14) recorded at a concentration of 2.0 mg L−1 after 6 weeks of culture. After two subcultures the multiplication rate was increased up to 19 shoots per explant on medium with 2iP (1.0 mg L−1). To prevent tissue browning, ascorbic acid and casein hydrolysate were added to the induction medium, resulting in a reduction of browning by 30%. The rooted plantlets were successfully transferred to soil and acclimatized with 97% frequency. Quantitative and qualitative assessments of oenothein B and phenolic acid contents in in vitro regenerated shoots as well as in ex vitro plants were performed using high-performance liquid chromatography with a diode-array detector (HPLC-DAD) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC MS/MS) methods. Oenothein B (1.62‒4.55 g 100 g−1 DW), ellagic acid, gallic and caffeic acids were identified in in vitro regenerated plants. The results of this study confirm that the oenothein B-producing plantlets can be obtained using the micropropagation method with axillary shoots being a valuable source of oenothein B and phenolic acids.

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A method for micropropagation of Cornus wilsoniana: An important biofuel plant

Cited by 15 publications

References 27 publications

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Micropropagation of Weigela florida ‘Tango’ through In Vitro Shoot Culture

Micropropagation and HPLC-DAD, UPLC MS/MS analysis of oenothein B and phenolic acids in shoot cultures and in regenerated plants of fireweed (Chamerion angustifolium (L.) Holub)

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