A method for quantitative characterization of growth in the 3-D structure of rat pulmonary arteries

Razavi, Hedi; Dusch, Marie N.; Zarafshar, Shahrzad Y.; Taylor, Charles A.; Feinstein, Jeffrey A.

doi:10.1016/j.mvr.2011.12.003

Cited by 25 publications

(32 citation statements)

References 24 publications

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“…Our data exhibited a similar trend to that of Lee et al The slightly larger values obtained by Lee et al were likely the result of the fact that their measurements were made in older rats (27). Sera et al (29) reported an average D/D P value of 0.89 for the rat airways, measured using microfocal X-ray CT, which is similar to our value of 0.80 (SD 0.22).…”

Section: Airwaysupporting

confidence: 90%

Airway and pulmonary vascular measurements using contrast-enhanced micro-CT in rodents

Counter

Wang

Farncombe

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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Counter WB, Wang IQ, Farncombe TH, Labiris NR. Airway and pulmonary vascular measurements using contrast-enhanced micro-CT in rodents. Am J Physiol Lung Cell Mol Physiol 304: L831-L843, 2013. First published April 5, 2013 doi:10.1152/ajplung.00281.2012.-Preclinical imaging allows pulmonary researchers to study lung disease and pulmonary drug delivery noninvasively and longitudinally in small animals. However, anatomically localizing a pathology or drug deposition to a particular lung region is not easily done. Thus, a detailed knowledge of the anatomical structure of small animal lungs is necessary for understanding disease progression and in addition would facilitate the analysis of the imaging data, mapping drug deposition and relating function to structure. In this study, contrast-enhanced micro-computed tomography (CT) of the lung produced high-resolution images that allowed for the characterization of the rodent airway and pulmonary vasculature. Contrast-enhanced micro-CT was used to visualize the airways and pulmonary vasculature in Sprague-Dawley rats (200 -225 g) and BALB/c mice (20 -25 g) postmortem. Segmented volumes from these images were processed to yield automated measurements of the airways and pulmonary vasculature. The diameters, lengths, and branching angles of the airway, arterial, and venous trees were measured and analyzed as a function of generation number and vessel diameter to establish rules that could be applied at all levels of tree hierarchy. In the rat, airway, arterial, and venous tress were measured down to the 20th, 16th, and 14th generation, respectively. In the mouse, airway, arterial, and venous trees were measured down to the 16th, 8th, and 7th generation, respectively. This structural information, catalogued in a rodent database, will increase our understanding of lung structure and will aid in future studies of the relationship between structure and function in animal models of disease. computed tomography; contrast agents; pulmonary circulation; airway nomenclature THE LUNG IS A COMPLEX ORGAN that works dynamically and must react to changes in posture, environment, and disease (20). Its major function is gas exchange, bringing oxygen into the body while removing carbon dioxide. To accomplish this task, a well-coordinated interaction between the lung and pulmonary circulation is essential. The rodent lung is comprised of two branching networks: the airways and pulmonary vasculature. With the advent of small animal imaging systems, researchers are able to visualize these networks in situ and noninvasively study the progression of lung disease and assess drug efficacy in animal models of disease. Much of this research involves combining the anatomical data obtained using computed tomography (CT) with the functional data obtained using single photon emission computed tomography (SPECT) or positron emission tomography (PET). A detailed knowledge of the structure of the lung would facilitate the mapping of functional data relative to lung anatomy and thus further our understandi...

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Section: Airwaysupporting

confidence: 90%

Airway and pulmonary vascular measurements using contrast-enhanced micro-CT in rodents

Counter

Wang

Farncombe

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Methods to provide contrast for the pulmonary arteries against the lung parenchyma in micro-CT imaging have been described previously (3,28). Briefly, pulmonary arteries were infused with a radiopaque contrast agent in banded (n ϭ 7) and sham-operated (n ϭ 6) animals.…”

Section: Arterial Castingmentioning

confidence: 99%

“…Fixed heart and lungs samples after arterial casting were imaged using two different micro-CT scanners (28). To validate the presence of the band around the LPA in the banded group, the fixed tissue samples were first evaluated under X-ray using an eXplore RS micro-CT scanner (GE Healthcare).…”

Section: High-resolution Micro-ct Imagingmentioning

confidence: 99%

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Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs

Razavi¹,

Stewart

Xu³

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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Pulmonary artery (PA) stenosis is a difficult obstructive defect to manage since clinicians cannot know a priori which obstructions to treat and when. Prognosis of PA stenosis and its chronic effects on lung development are poorly understood. This study aimed to characterize the hemodynamic and structural effects of PA stenosis during development. Fourteen male Sprague-Dawley rats underwent left PA (LPA) banding at age 21 days, and 13 underwent sham operation. Hemodynamic and structural impacts were studied longitudinally at 20, 36, 52, 100, and 160 days. Chronic LPA banding resulted in a significant reduction in LPA flow (P < 0.0001) and size of both proximal LPA (P < 0.0001) and distal LPA (P < 0.01), as well as a significant increase in flow and size of the right PA (P < 0.05) throughout development. Flows and sizes adapted such that normal levels of wall shear were restored after banding. At 160 days, LPA banding resulted in a significant decrease in left lung volume and an increase in right lung volume but no significant differences in total lung volume. There was an elevation of proximal LPA pressure as well as right ventricular hypertrophy in the banded animals. The banded lung exhibited arterial disorganization, loss of vessels, and enlargement of its bronchial arteries, whereas the contralateral lung showed signs of vascular pathology. There are consequences on development of both lungs in the presence of an LPA stenosis at young age. These results suggest that early intervention may be necessary to optimize left lung growth and minimize right lung vascular pathology.

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“…The latter phenomenon is also important for the understanding of tumor growth and could be used in oncological treatment strategies, especially in patients treated by biological therapy with antibodies against vascular endothelial factor A (bevacizumab), which inhibits neoangiogenesis . Micro-CT imaging of pathological vascularity can provide new information, e.g., about changes in vessel walls in atherosclerosis or other pulmonary vascular diseases (Razavi, 2012).…”

Section: Current Applications Of Micro-ct In Biomechanics and Medicinementioning

confidence: 99%

Correlating Micro-CT Imaging with Quantitative Histology

Gregor¹,

Kochová²,

Eberlová³

et al. 2012

Injury and Skeletal Biomechanics

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A method for quantitative characterization of growth in the 3-D structure of rat pulmonary arteries

Cited by 25 publications

References 24 publications

Airway and pulmonary vascular measurements using contrast-enhanced micro-CT in rodents

Airway and pulmonary vascular measurements using contrast-enhanced micro-CT in rodents

Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs

Correlating Micro-CT Imaging with Quantitative Histology

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