A Method for Quick Determination of Bile Acids in Bile of Patients with Biliary Lithiasis

Coca, Enrique López de; Ribas, B.; Trigueros, G.; Sarmiento, Juan M.; Borque, M.; Ortega, Dolores; Sobrino, Agua; Mallen, Adrianne; Pablos, I. De; Fdez-Represa, J. Alvarez

doi:10.1080/10826079408013768

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…Analysis of conjugated bile salts was performed essentially according to Coca et al (1994). To 100 μL of bile, 5 μL of internal standard (dexamethasone, 20 mg mL −1 ) was added before diluting 50‐fold with 0.7 M phosphate buffer (pH 7.0).…”

Section: Methodsmentioning

confidence: 99%

Digestibility ofCalanus finmarchicuswax esters in Atlantic salmon (Salmo salar) freshwater presmolts and seawater postsmolts maintained at constant water temperature

Oxley

Bogevik

Henderson

et al. 2009

Aquaculture Nutrition

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Calanoid copepods are a rich source of marine lipid for potential use in aquafeeds. Copepod oil is primarily composed of wax esters (WE) and there are concerns over the efficiency of wax ester, versus triacylglycerol (TAG), digestion and utilization in fish. As smoltification represents a period of major physiological adaptation, the present study examined the digestibility of a high WE diet (Calanus oil; 48% WE, 26% TAG), compared with a TAG diet (fish oil; 58% TAG), in Atlantic salmon freshwater presmolts and seawater postsmolts, of similar age (9 months) and weight (112 g and 141 g initial, respectively), over a 98‐day period at constant temperature. Fish grew significantly better, and possessed lower feed conversion ratios (FCR), in seawater than freshwater. However, total lipid apparent digestibility coefficient (ADC) values were significantly lower in seawater fish, as were total fasted bile volumes. Dietary Calanus oil also had a significant effect, reducing growth and lipid ADC values in both freshwater and seawater groups. Postsmolts fed dietary Calanus oil had the poorest lipid ADC values and analysis of faecal lipid class composition revealed that 33% of the remaining lipid was WE and 32% fatty alcohols. Dietary prevalent 22:1n‐11 and 20:1n‐9 fatty alcohols were particularly poorly utilized. A decrease in primary bile acid, taurocholate, concentration was observed in the bile of dietary Calanus oil groups which could be related to the lower cholesterol content of the diet. The dietary WE : TAG ratio is discussed in relation to life stage and biliary intestinal adaptation to the seawater environment postsmoltification.

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Section: Methodsmentioning

confidence: 99%

Digestibility ofCalanus finmarchicuswax esters in Atlantic salmon (Salmo salar) freshwater presmolts and seawater postsmolts maintained at constant water temperature

Oxley

Bogevik

Henderson

et al. 2009

Aquaculture Nutrition

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“…Chemical transformations of bile acids and determination in biological materials have challenged researchers to pay attention on their biosynthesis or develop various analytical methods [19,20,21,22,23]. The HPLC method is quite simple and punctilious.…”

Section: Discussionmentioning

confidence: 99%

Concentrations of sodium 3α, 7α--dihydroxy-12-oxo 5β cholanate in biological material after its intravenous and intranasal application

Stević¹,

Mikov²,

Stanojević-Ristić³

et al. 2017

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Newly synthetized derivative of bile acid, sodium salt of 3α, 7α-dihydroxy-12-oxo 5β cholanic acid (monoketocholanate) expressed a good characteristic as intranasal transport enhancer of xenobiotics.The aim of our sudy was to explore if it has an influence on bile metabolism and to measure its concentration in blood and bile after intravenous and intranasal administration. The experiment was performed in vivo on adult male Wistar rats. The determination of monoketocholanate (MKCh) in rats blood and bile, was carried out by high-performance liquid chromatography (HPLC), on an HP ODS2 column, using methanol/acetonitrile/acetate buffer as mobile phase. Absorbances were measured at 210 nm.Blood samples were taken from the prepared right axillary artery in 0, 1, 5, 10, 20, 30, 60, 90, 120 and 180 minutes from the beginning of the experiment. Bile was collected in a half an hour intervals,during the three hour period. The results showed that MKCh changed the amount of excreted bile depending on the way of application. Intranasal application increased the bile volume and the MKCh concentration, both in blood and bile compared to the intravenous application (p<0.05). Distributionm of MKCh through animal organism depends on the way of application of the substance, which probably determines its caracterisation as the transport promotor of applied xenobiotics. HPLC has proved as aa relatively simple, fast and effective method for the determination of synthetic bile acid,MKCh in these biological materials. key words: bile acids and salts, HPLC method, intranasal and intravenous administration, rats

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“…A modification of the HPLC procedure described by Scalia (1988) was used to determine BSH activity. Traditionally, in-vitro bile acid experimentation has involved the use of HPLC to determine the quantity of various tauro-and glycol-bile acids in complex mixtures of added bile acids in complex aqueous media (Cantafora et al 1987, Coca et al 1994, Scalia 1988). Methods of separating such mixtures have required a lengthy workup involving a (1:4; v:v) sample:isopropanol extraction followed by evaporation and resuspension steps (Cantafora et al 1987, Coca et al 1994, Scalia 1988.…”

Section: Bile Salt Hydrolase Assaymentioning

confidence: 99%

“…Traditionally, in-vitro bile acid experimentation has involved the use of HPLC to determine the quantity of various tauro-and glycol-bile acids in complex mixtures of added bile acids in complex aqueous media (Cantafora et al 1987, Coca et al 1994, Scalia 1988). Methods of separating such mixtures have required a lengthy workup involving a (1:4; v:v) sample:isopropanol extraction followed by evaporation and resuspension steps (Cantafora et al 1987, Coca et al 1994, Scalia 1988. While this method can produce accurate results, we eliminated the time-consuming and labor-intensive workup step of evaporation, allowing for an efficient workup while preserving the quality of bile acid separation and quantification.…”

Section: Bile Salt Hydrolase Assaymentioning

confidence: 99%

Deconjugation of Bile Acids with Immobilized Genetically EngineeredLactobacillus plantarum80(pCBH1)

Jones

Martoni

Chen

et al. 2005

Applied Bionics and Biomechanics

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Bile acids are important to normal human physiology. However, bile acids can be toxic when produced in pathologically high concentrations in hepatobileary and other diseases. This study shows that immobilized genetically engineered Lactobacillus plantarum 80 (pCBH1) (LP80 (pCBH1)) can efficiently hydrolyze bile acids and establishes a basis for their use. Results show that immobilized LP80 (pCBH1) is able to effectively break down the conjugated bile acids into glycodeoxycholic acid (GDCA) and taurodeoxycholic acid (TDCA) with bile salt hydrolase (BSH) activities of 0.17 and 0.07 µmol DCA/mg CDW/h, respectively. The deconjugation product, deoxycholic acid (DCA), was diminished by LP80 (pCBH1) within 4 h of initial BSH activity. This in-vitro study suggests that immobilized genetically engineered bacterial cells have important potential for deconjugation of bile acids for lowering of high levels of bile acids for therapy.

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A Method for Quick Determination of Bile Acids in Bile of Patients with Biliary Lithiasis

Cited by 7 publications

References 12 publications

Digestibility ofCalanus finmarchicuswax esters in Atlantic salmon (Salmo salar) freshwater presmolts and seawater postsmolts maintained at constant water temperature

Digestibility ofCalanus finmarchicuswax esters in Atlantic salmon (Salmo salar) freshwater presmolts and seawater postsmolts maintained at constant water temperature

Concentrations of sodium 3α, 7α--dihydroxy-12-oxo 5β cholanate in biological material after its intravenous and intranasal application

Deconjugation of Bile Acids with Immobilized Genetically EngineeredLactobacillus plantarum80(pCBH1)

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