A method for semiautomatic differential analysis of urinary catecholamines

Hathaway, Paul W.; Jakoi, Laszlo; Troyer, William G.; Bogdonoff, Morton D.

doi:10.1016/0003-2697(67)90291-6

Cited by 11 publications

(2 citation statements)

References 8 publications

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“…The assay was sensitive over the range 0.04-1 .OO nmol. Catecholamines were determined by a combined technique of Hathaway et al (1967) and Chang (1964). 5-Hydroxytryptamine (5-HT, serotonin) was detected according to Snyder et al (1965).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Rapid Preparation of Synaptosomes from Mammalian Brain Using Nontoxic Isoosmotic Gradient Material (Percoll)

Nagy¹,

Delgado‐Escueta²

1984

Journal of Neurochemistry

325

166

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A new procedure is described for the isolation of synaptosomes from various parts of mammalian brain. This method utilizes an isoosmotic Percoll/sucrose discontinuous gradient and has some advantages over the traditionally used synaptosomal isolation techniques: (1) it is possible to prepare suitable gradients while retaining isoosmolarity; (2) the time of the preparation is remarkably short (approximately 1 h); (3) if necessary, the gradient material can be easily removed from the samples. Intact synaptosomes were recovered from the 10%/16% (vol/vol) Percoll interphase. The fractions were identified and characterized by electron microscopy and by several biochemical markers for synaptosomes and other subcellular organelles. The homogeneity of the preparations is comparable to or better than that of synaptosomes prepared by the conventional methods. This procedure has been successfully used for the isolation of synaptosomes from very small tissue samples of various experimental animals and human brain.

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Section: Enzyme Assaysmentioning

confidence: 99%

Rapid Preparation of Synaptosomes from Mammalian Brain Using Nontoxic Isoosmotic Gradient Material (Percoll)

Nagy¹,

Delgado‐Escueta²

1984

Journal of Neurochemistry

325

166

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“…Urinary NE and E measurements were performed, as described by Hathaway et a/., 10 involving alumina elution and assay with an AutoAnalyzer* by a method utilizing the trihydroxyindole reaction. Urine osmolality and creatinine levels were also determined.…”

Section: Vol XXXI No I 1969mentioning

confidence: 99%

Urine Flow, Catecholamines, and Blood Pressure

Hathaway

Brehm

Clapp

et al. 1969

Psychosomatic Medicine

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The relationship of catecholamines, urine flow, blood pressure, and other variables were studied in 34 healthy college males placed alone in a laboratory relatively free of external stimuli. Urine was collected pre and post ingestion of water, and the urine flow was found to vary widely in a single individual, both pre and post ingestion of water. The initial urinary flow and excretion rates of norepinephrine (NE) and epinephrine (E) correlated with the degree of water diuresis which occurred within 1 hr. after ingestion of water. The urinary excretion rates for NE and E were also positively correlated with the magnitude of urine flow following water ingestion as well as with systolic and diastolic blood pressure. The heart rate was positively correlated only with E excretion.The baseline NE excretion of smokers was greater than that of nonsmokers. The baseline urine flow of smokers was one-half that of nonsmokers.Serial Nowlis mood-adjective check lists revealed that subjects with a high urine flow checked less anxiety adjectives on arrival. They also checked more adjectives for pleasant feelings at the end of the study and showed an increase in generalized activation items.In view of the recent findings that urinary excretion rates of catecholamines are independent of urine flow and urine pH, it is suggested that the high rate of catecholamine excretion associated with increased urine flow is indicative of elevated plasma levels. These data suggest an interrelationship between autonomic nervous system activity and the response of the individual to an imposed water load.Our most striking finding is that there is a wide variation of response to a simple laboratory atmosphere and that individual differences, present at the very onset of a study, in part determine the subjects' urine flow rates, feeling states, catecholamine excretions, and cardiovascular responses to the laboratory environment and to water ingestion. T Aw -ms STUDY WAS UNDERTAKEN to evalu-tion of intrasubject urine flow rates, ate the phenomenon of the wide varia-often encountered in experimental situa-

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Transfer From a Coronary Care Unit

Klein¹

1968

Arch Intern Med

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A method for semiautomatic differential analysis of urinary catecholamines

Cited by 11 publications

References 8 publications

Rapid Preparation of Synaptosomes from Mammalian Brain Using Nontoxic Isoosmotic Gradient Material (Percoll)

Rapid Preparation of Synaptosomes from Mammalian Brain Using Nontoxic Isoosmotic Gradient Material (Percoll)

Urine Flow, Catecholamines, and Blood Pressure

Transfer From a Coronary Care Unit

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