A Method for testicular denervation in rats

Kullendorff, Carl‐Magnus; Ivarsson, H.; Ekelund, Mats; Holmin, T.

doi:10.1002/micr.1920030407

Cited by 5 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the method it is possible to identify and selec tively resect the thin nerve bundles and fibres in the hepato-duodenal ligament. A similar technique has been previously developed for identification of the nerves running close to the spermatic vessels in the same species [7,9]. From a technical and microsurgical point of view, these new techniques represent valu able contributions for the elucidation of the nervous regulation of different functions in the parenchymatous organs of small animals.…”

Section: Discussionmentioning

confidence: 99%

A Microsurgical Method for Denervation of the Liver in the Rat

Holmin¹,

Ekelund²,

Kullendorff

et al. 1984

Eur Surg Res

Self Cite

View full text Add to dashboard Cite

The role of the nerves in the hepato-duodenal ligament should be of special surgical interest, since these nerves are often injured in hepatic and biliary tract surgery. We have developed a microsurgical procedure for liver denervation. All visible nerves were resected after staining. Norepinephrine values in central liver tissue of denervated rats were only 18 % of the corresponding values in innervated liver tissue, indicating an appreciable degree of sympathetic denervation. No detectable immunoreactivity of gastrin, cholecystokinin, vasoactive intestinal polypeptide, substance P, somatostatin or enkephalin were found within resected hepatic nerves. The described denervation procedure is simple and reproducible. It can be used to obtain additional information about the nervous regulation of various liver functions.

show abstract

Section: Discussionmentioning

confidence: 99%

A Microsurgical Method for Denervation of the Liver in the Rat

Holmin¹,

Ekelund²,

Kullendorff

et al. 1984

Eur Surg Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Species variation may be important. There is very little evidence of a pronounced difference in blood loss in humans between those techniques that advocate extrahepatic dissection [4], and those which concentrate on intrahepatic vascular access [5]. On the other hand, denervation may be very much more important for the transplanter, who will be working with a truly denervated model.…”

Section: Griffith and Emerymentioning

confidence: 99%

“…Such a dissection may involve the unintentional division of most of the hepatic nerves. A recently developed microsurgical method for denervation of the spermatic vessels in the rat [3,4] was adapted for denervation of the rat liver [5]. The method facilitates identification and selective resection of the majority of the nerves within the hepatoduodenal ligament.…”

mentioning

confidence: 99%

Excessive bleeding at hepatic resection after experimental liver denervation

et al. 1984

Self Cite

View full text Add to dashboard Cite

The effects of microsurgical denervation of the liver on blood loss, bleeding time, platelet count, and partial thromboplastin time (APT‐time) after standardized liver resection were studied in the rat. The hepatic nerves in the hepatoduodenal ligament were dyed, identified, and resected with the aid of a microsurgical technique. During the same anesthesia and also one week after denervation, a 3‐ to 4‐fold increase in blood loss and bleeding time was demonstrated when compared to sham‐operated and control rats. As a result of the increased blood loss, hemoglobin and hematocrit values fell, whereas platelet count and APT‐time were essentially unchanged. The denervation procedure had no influence on platelet aggregation. In all probability, the increased bleeding after hepatic resection in the denervated rats is a reflection of an interrupted nerve supply to the liver. The results may have interesting implications for human liver surgery.

show abstract

A microsurgical method for experimental kidney denervation

Kullendorff

1986

Microsurgery

View full text Add to dashboard Cite

A successful microsurgical method for denervation of the kidney in the rat is presented. Unilateral kidney denervation was performed in eight animals. Ten sham-operated animals were used as controls. Under ether anaesthesia, the kidney vessels on one side were exposed. The peritoneum above the vessels was incised and the area was stained with 1% toluidine blue solution. The nerves were identified. With microsurgical technique, the nerves were carefully separated from the vessels and resected. Tissue specimens from both kidneys in all animals were removed and analysed for norepinephrine content. There was no normal difference between the kidneys in the sham-operated rats. About 1 week after denervation, the norepinephrine concentration in the denervated kidney showed a 95% reduction, indicating a complete denervation. The described microsurgical procedure was a rapid, simple, and reproducible method for kidney denervation in the rat.

show abstract

A Method for testicular denervation in rats

Cited by 5 publications

References 5 publications

A Microsurgical Method for Denervation of the Liver in the Rat

A Microsurgical Method for Denervation of the Liver in the Rat

Excessive bleeding at hepatic resection after experimental liver denervation

A microsurgical method for experimental kidney denervation

Contact Info

Product

Resources

About