A method for the analysis of six thyroid hormones in thyroid gland by liquid chromatography–tandem mass spectrometry

Kunisue, Tatsuya; Fisher, Jeffrey W.; Fatuyi, Babatope

doi:10.1016/j.jchromb.2010.04.031

Cited by 29 publications

(19 citation statements)

References 36 publications

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“…In addition to routine laboratory quality controls, a pool of serum from the cohort was created at the beginning of the study and 2 aliquots, undistinguishable from the test samples were inserted in each laboratory run. Sex steroids were quantified by the Liquid Chromatography Tandem Mass Spectrometry on an Applied Biosystems API4000 triple stage quadrupole mass spectrometer, which has demonstrated good laboratory performances as supported by other studies (24–27). Laboratory quality controls most closely corresponding to the levels observed in the population: 0.1 ng/mL for testosterone, 5.0 ng/mL for androstenedione, 5.0 ng/mL for 17-OH-progesterone, 75.0 ng/mL for progesterone, and 5.0 ng/mL for estradiol, showed inter-run coefficients of variation (CV) of 14.6%, 5.3%, 5.0%, 5.0% and 8.3%, respectively.…”

Section: Methodsmentioning

confidence: 80%

Circulating Sex Steroids during Pregnancy and Maternal Risk of Non-epithelial Ovarian Cancer

Chen

Surcel

Lundin

et al. 2011

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background Sex steroid hormones have been proposed to play a role in the development of non-epithelial ovarian cancers (NEOC) but so far no direct epidemiological data are available. Methods A case-control study was nested within the Finnish Maternity Cohort, the world’s largest bio-repository of serum specimens from pregnant women. Study subjects were selected among women who donated a blood sample during a singleton pregnancy that led to the birth of their last child preceding diagnosis of NEOC. Case subjects were 41 women with sex-cord stromal tumors (SCST) and 21 with germ cell tumors (GCT). Three controls, matching the index case for age, parity at the index pregnancy, and date at blood donation were selected (n=171). Odds ratios (OR) and 95% confidence intervals (CI) associated with concentrations of testosterone, androstenedione, 17-OH-progesterone, progesterone, estradiol and sex hormone binding globulin (SHBG) were estimated through conditional logistic regression. Results For SCST, doubling of testosterone, androstenedione and 17-OH-progesterone concentrations were associated with about 2-fold higher risk of SCST [ORs and 95% CI of 2.16 (1.25–3.74), 2.16 (1.20–3.87), and 2.62 (1.27–5.38), respectively]. These associations remained largely unchanged after excluding women within 2, 4 or 6 years lag-time between blood donation and cancer diagnosis. Sex steroid hormones concentrations were not related to maternal risk of GCT. Conclusions This is the first prospective study providing initial evidence that elevated androgens play a role in the pathogenesis of SCST. Impact Our study may note a particular need for larger confirmatory investigations on sex steroids and NEOC.

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Section: Methodsmentioning

confidence: 80%

Circulating Sex Steroids during Pregnancy and Maternal Risk of Non-epithelial Ovarian Cancer

Chen

Surcel

Lundin

et al. 2011

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…The method is based on solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for separation and detection. Recently, the applicability of SPE and LC-MS/MS to the analysis of thyroid hormones in the thyroid gland was successfully demonstrated [17], however, to the best of our knowledge this is the first method for the simultaneous quantitative analysis of these compounds in the different water matrices.…”

Section: Introductionmentioning

confidence: 99%

Analysis of thyroid hormones in raw and treated waste water

Svanfelt

Eriksson

Krönberg

2010

Journal of Chromatography A

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“…This intention is somehow surprising since in humans it is assumed that only approximately 20% of circulating T 3 originates directly from the thyroid [7], while the thyroidal secretion of the 'inactive' rT 3 is insignificant [8]. There are descriptions of endogenous T 2 and rT 3 tissue levels using less sensitive methodologies [3,9,10].…”

Section: Discussion/significance Of the Resultsmentioning

confidence: 99%

“…The scientific advancement of this method-oriented work merely detecting tissue T 4 and T 3 levels is confined, since other publications using LC-MS/MS or immunological methods have already reported tissue TH profiles in more detail [2][3][4].…”

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confidence: 99%

LC–MS/MS detection of thyroid hormone metabolites in tissue samples

Hoefig

Köhrle

2012

Expert Review of Endocrinology & Metabolism

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The recent article by Ackermans et al., published in Biomedical Chromatography [1] , describes the liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based determination of five thyroid hormone (TH) metabolites (T 4 , T 3 , rT 3 , 'rT 2 ' [i.e., 3,5-T 2 ], and T 2 [3,3´-T 2 ]) within one analytical run in samples from different tissues. The aim of the article is to use this technique to better understand tissue-specific TH metabolism. Unfortunately, the authors used an unusual and even misleading abbreviation for 3,5-T 2 (they call it "rT 2 "). In the context that rT 3 is frequently used as abbreviation for the 'reverse' 3,3´,5´-triiodothyronine isomer, which is devoid of T 3 -like thyromimetic activity, one would intuitively assume that "rT 2 " might be a direct 3-deiodination product of rT 3 . However, this is chemically impossible and not the case because 3,5-T 2 , named "rT 2 " by the authors can only be formed from T 3 by 3´-deiodination. Only two endogenous hormones, namely T 4 and T 3 , were detected above the limit of quantification in the tissue samples analyzed; however, the detection of other endogenous TH metabolites would be desirable. Further preanalytical sample workup www.expert-reviews.com Key Paper Evaluation Key issues• A liquid chromatography-tandem mass spectrometry method was used to measure five thyroid hormone (TH) metabolites in one analytical run. • Four deuterated standards were used for the validation of TH quantification.• T 4 and T 3 were detected only qualitatively but not quantified in tested rat tissue samples; other metabolites were not detected at all.• The possibility to link tissue TH levels to local iodothyronine deiodinase expression is proposed.• However, the issues relating to the contribution of carrier-mediated TH import and export to local concentration analyzed are not addressed.

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A method for the analysis of six thyroid hormones in thyroid gland by liquid chromatography–tandem mass spectrometry

Cited by 29 publications

References 36 publications

Circulating Sex Steroids during Pregnancy and Maternal Risk of Non-epithelial Ovarian Cancer

Circulating Sex Steroids during Pregnancy and Maternal Risk of Non-epithelial Ovarian Cancer

Analysis of thyroid hormones in raw and treated waste water

LC–MS/MS detection of thyroid hormone metabolites in tissue samples

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