A Method for the Production of Stress Erosion in the Mouse Stomach and Related Pharmacological Studies

Abstract-The influences of restraint stress on the functions of T cells, B cells and adherent cells in antibody responses were investigated. Antibody response against sheep red blood cells (SRBC), a T cell-dependent antigen, in cultured splenocytes from restrained mice was reduced to about 40-50% of that from the control mice. Addition of normal T cells to these cultures, however, restored the suppressed response.Moreover, helper T cell activities were lowered in restrained mice. On the other hand, suppressor T cell activities induced by both concanavalin A (Con A) and SRBC were significantly decreased in restrained mice. However, the antibody responses to T cell-independent antigens in stressed mice were approximately 40% higher than the control response. These enhancemant were also observed in T cell-depleted splenocytes. Polyclonal antibody response induced by lipopolysaccharide (LPS) was increased in stressed mice. Antigen presenting cell activities were little influenced by restraint stress. Proliferative response to Con A, but not that to LPS, was suppressed in splenocytes from restrained mice. These results suggest that both helper and suppressor activities of T cells are suppressed, but B cell activity is rather enhanced in splenocytes from restrained mice.

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“…The restraint cages were prepared according to the literature (17). Usually, mice were restrained for 2 con secutive days.…”

Section: Tamatsukurimentioning

confidence: 99%

Stress and Immune Responses II. Identification of Stress-Sensitive Cells in Murine Spleen Cells

Okimura

Satomi-Sasaki

Ohkuma

1986

Japanese Journal of Pharmacology

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“…Restraint and water immersion stress produces a high incidence and severity of gastric lesions in rats (1, 2) and mice (3,4). Exposure of rats to this stress induced an increase in gastric motility (5), and thus the increased gastric motility as well as the presence of gastric juice (6) and the disorder of gastric mucosal blood flow (7,8) was postulated to play an im portant role in forming stress-induced gastric lesions (9,10).…”

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confidence: 99%

Role of Gastric Motility in Development of Stress-Induced Gastric Lesions of Rats

Yano

Akahane

Harada

1978

Japanese Journal of Pharmacology

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Abstract-Gastric motility of stressed rats was studied to determine its role in producing stress-induced gastric lesions. Restraint and water immersion resulted in an increase in gastric motility which consisted of an increase in frequency and amplitude of con tractions and a rise in gastric tone.This increase reached maximal levels 2 to 4 hr after stress, and persisted thereafter. Restraint and water immersion stress produces a high incidence and severity of gastric lesions in rats (1, 2) and mice (3, 4). Exposure of rats to this stress induced an increase in gastric motility (5), and thus the increased gastric motility as well as the presence of gastric juice (6) and the disorder of gastric mucosal blood flow (7, 8) was postulated to play an im portant role in forming stress-induced gastric lesions (9, 10). However, there is apparently no documentation demonstrating a causal relationship between gastric motility changes and stress ulcer formation.Gastric motility is increased by stress through vagal activity which becomes more prominent than sympatho-adrenal activity (11,12). Therefore, gastric motility may vary in its increasing pattern depending on the stress since intensity and duration of stress affect the activation of vagal tone in a different manner.The present study was designed to determine if gastric motility changes were associated with the formation of gastric lesions in stress and, if so, to determine whether this involve ment was a main cause for the pathogenesis of stress-induced gastric lesions. MATERIALS AND METHODSMale Wistar rats weighing 240 to 280 g were deprived of food for 18 hr, but permitted water ad libitunz.Two procedures were used to induce stress gastric lesions.In the first procedure, animals were restrained in firmly fitted restraint cages and placed in a room of 22-23'C.

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“…These and subsequent studies have suggested that enhanced gastric secretion (7) and/or motility (8,9) are important in the etiology of stress ulcer, giving a theoretical basis for the development of antiulcer drugs that suppress gastric secretion.…”

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confidence: 99%

Sulpiride Specifically Attenuates Psychological Stress-Induced Gastric Lesions in Rodents

Nomura¹,

Maeda²,

Kuratani³

et al. 1995

Japanese Journal of Pharmacology

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ABSTRACT-Gastric lesions were developed in the communication box paradigm (CB) in mice as well as in the activity-stress paradigm (AS) in rats. Treatment with sulpiride (10-320 mg/kg, p.o.) attenuated these psychological stress-induced gastric lesions in a dose-dependent manner, while it failed to suppress those induced by physical stress such as restraint water-immersion (WI) and indomethacin treatment (IND). In contrast, treatment with famotidine (0.32-10 mg/kg, p.o.) dose-dependently attenuated the gastric lesions induced by physical stress but not those by psychological stress. Pylorus-ligation study revealed that famotidine strongly reduced gastric acid secretion, whereas sulpiride minimally affected that. It was also demonstrated that physical stress (WI) enhanced acid secretion while psychological stress (CB and AS) rather depressed that. These results suggest that the mechanisms of gastric lesion formation are clearly different between physical and psychological stress and that sulpiride specifically attenuates psychological stress lesions possibly through a central mechanism.

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A Method for the Production of Stress Erosion in the Mouse Stomach and Related Pharmacological Studies

Abstract: Abstract-A stress method in mice which develops gastric erosions was devised. This stress procedure consisted of restraint and water immersion.

Cited by 31 publications

References 5 publications

Stress and Immune Responses II. Identification of Stress-Sensitive Cells in Murine Spleen Cells

Stress and Immune Responses II. Identification of Stress-Sensitive Cells in Murine Spleen Cells

Role of Gastric Motility in Development of Stress-Induced Gastric Lesions of Rats

Sulpiride Specifically Attenuates Psychological Stress-Induced Gastric Lesions in Rodents

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