Abstract:⎯A method was developed for the simultaneous detection of V. cholerae strains and the presence of drug-resistant genes in their genomes by real-time PCR. Resistance to four antibiotics used for cholera treatment (tetracycline, trimethoprim, chloramphenicol and ciprofloxacin) was analyzed. The sensitivity of the panel was 1 × 10 3 CFU/mL for pure cultures, simulated clinical material, and environmental samples. PCR efficiency was confirmed by the analysis of 60 natural V. cholerae strains isolated from patients… Show more
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