A Method for the Simultaneous Separation of Human Thrombocytes and Leucocytes

Uptake of Labelled Antigens by Lymphocytes of Rabbits with Delayed Hypersensitivity Reactions

“…The washed, white cells were sus pended in 10 drops of Eagle's medium (without scrum), and 5 drops of labelled antigen (2 mg protein per ml) were added. After incubation at 37"C for 30 min the few remain ing red cells were hemolized by distilled water lysis as described by Nelken et al (10). The cells were shaken for 20 sec with 3 ml of distilled water, after which 1 ml of a 3.4 percent NaCl solution was immediately added.…”

“…After about 45 min at 3 7 0 the clear supernatant, containing white cells and a few red cells, could be transferred to smaller tubes and washed twice. To avoid agglutination of the white cells saline with 10% of the F.DTA solution with I 0,, WR-1339, recommended by Nei.ken et al (10). was used as washing medium.…”

A Two-Stage Agglutination Test for the Detection of Antithrombocyte Antibodies

“…Human thrombo cytes, separated from whole blood by differential centrifugation [10] and washed four times, were injected subcutaneously and intramuscularly into chickens weighing 1.5-2.0 kg, at 5-day intervals. The first four injections contained an equal volume of complete Freund's adjuvant ; three further injections were given without adjuvant.…”

Effect of Hemin and Protoporphyrin IX on the Protein-Synthesizing Activity of Human Granulocytes, Lymphocytes and Platelets

“…It consisted mainly of a mixture of granulocytes, lymphocytes and plate lets and was separated into three equal portions, used for the preparation of pure cell populations. Granulocytes were separated by the method of Skoog and Beck [17]; subsequent hemolysis of the residual red blood cells was carried out according to Nelken et al, [12], Lymphocytes were separat ed by the Ficoll-Hypaque procedure [18], and the platelets, as previously described [2]. Sterile condi tions were maintained throughout.…”