A Method to Assess Histamine-Induced Cyclic AMP Production in Isolated Gastric Mucosal Cells from Human Biopsies

Sarem-Aslani, A.; Ratge, D.; Bigge, H.-H.; Walker, S.; Klotz, Ulrich; Wisser, H.

doi:10.1159/000138815

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…As a result, the combination of the choices of supportive bedding and enriched media, determine the resulting cellular composition (reviewed in Table 1), as compared to simple cell culture systems. 10,12,[14][15][16]18,46,[60][61][62] Therefore, we believe that our combination of MEF bedding and the 2D enriched media yield a cellular composition that includes epithelial, gastric-specific, and progenitor cells.…”

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

A simple and cost‐efficient adherent culture platform for human gastric primary cells, as an in vitro model for Helicobacter pylori infection

et al. 2018

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Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

A simple and cost‐efficient adherent culture platform for human gastric primary cells, as an in vitro model for Helicobacter pylori infection

et al. 2018

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“…Gastric mucosal cells were prepared according to the methods of Mardh et al [30] and Sarem-Aslani et al [31] under sterile conditions. 5-7 biopsies were tak en endoscopically from antral mucosa and were trans ferred to a thermostated glass tube containing 3 mg pronase (from Streptomyces griseus: Boehringer, Mannheim) in 2 ml medium I (70 mmol/1 NaCl.…”

Section: Isolation O F Human Gastric Mucosal Cellsmentioning

confidence: 99%

Interaction between <i>Helicobacter pylori and </i>Human Gastric Epithelial Cells in Culture: Effect of Antiulcer Drugs

Wagner¹,

Beil²,

U³

et al. 1994

Pharmacology

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A human in vitro model to study the interaction between Helicobacter pylori and gastric epithelial cells was developed using primary cultures of gastric mucosal cells (isolated from gastric biopsies or operative specimen and maintained in culture for 2 weeks) as well as the well-differentiated human gastric carcinoma cell line HM02, the undifferentiated gastric tumour cell line HM51, and the laryngeal epithelial cell line HEp-2. Primary cultures and all cell lines were exposed to seven isolates of H. pylori isolated from gastritis and duodenal ulcer patients. Microbial adherence was assessed by microscopical evaluation of Giemsa-stained preparations and by culturing the viable bacteria attached to the epithelial cells. All H. pylori isolates adhered to the gastric cells in primary culture, to HM02 cells, and to HEp-2 cells with the greatest binding affinity found in primary gastric cells. No adherence was detected in HM51 cells. H. pylori adherence was dependent on bacterial load, incubation time, and temperature. There was no difference in microbial binding between H. pylori isolates derived from gastritis and duodenal ulcer patients. The effect of antiulcer drugs on H pylori adherence was investigated by pre-incubating isolates of H. pylori with omeprazole, cimetidine, and bismuth subcitrate. Omeprazole and cimetidine failed to significantly influence microbial adherence. In contrast, bismuth subcitrate already in concentrations below the MIC range decreased H. pylori adherence in gastric epithelial cells and in HEp-2 cells substantially. Our study shows that primary cultured human gastric mucosal cells and the human gastric carcinoma cell line HM02 provide suitable in vitro models for the study of the interactions between H. pylori and the gastric epithelium. This gastric cell model is characterized by a high affinity for H. pylori binding.

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Isolation, identification and quantitative evaluation of specific cell types from the mammalian gastric mucosa

et al. 1993

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Functional in vitro studies with isolated gastric mucosal cells require cytological identification of different cell types in suspension or primary culture. Since suitable techniques have not been well established, different staining methods for the discrimination of dispersed pig and guinea pig gastric cells have been developed on the basis of modified previous protocols for enzymatic cell dispersion. Chief and parietal cells were visualized by combined periodic acid-Schiff stains. Surface mucous and mucous neck cells were identified by affinity-labelling, using lectins with selective staining properties in situ. Two of the lectins were found to be specific markers for gastric polymorphonuclear cells. The following vital tests were found to be useful: succinic dehydrogenase for parietal cells, Nile blue/brilliant cresyl blue stains for chief cells, and different phagocytosis assays for endothelial cells and gastric phagocytes. Endocrine cells were characterized by immunocytochemistry using specific antibodies against gastrin, somatostatin, histamine and serotonin. The same technique using a vimentin antibody was performed for the identification of fibroblasts. Proliferation of mucosal cells in primary culture was monitored by the incorporation of bromo-deoxyuridine, which was subsequently detected by a monoclonal antibody.

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A Method to Assess Histamine-Induced Cyclic AMP Production in Isolated Gastric Mucosal Cells from Human Biopsies

Cited by 5 publications

References 15 publications

A simple and cost‐efficient adherent culture platform for human gastric primary cells, as an in vitro model for Helicobacter pylori infection

A simple and cost‐efficient adherent culture platform for human gastric primary cells, as an in vitro model for Helicobacter pylori infection

Interaction between <i>Helicobacter pylori and </i>Human Gastric Epithelial Cells in Culture: Effect of Antiulcer Drugs

Isolation, identification and quantitative evaluation of specific cell types from the mammalian gastric mucosa

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