A method to establish antibody secreting B cell lines and simultaneously perform frequency analysis

Zhang, J W; Henderikx, Paula; Cong, Ying; Medaer, Robert; Raus, Jef

doi:10.1016/0022-1759(89)90042-2

Cited by 9 publications

References 8 publications

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Pico/Nanoliter Chamber Array Chips for Single‐cell,DNAand Protein Analyses

Yamamura

Sathuluri

Tamiya

2003

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction Multiplexed Polymerase Chain Reaction from A Single Copy DNA using Nanoliter‐volume Microchamber Array PCR Microchamber Array Chip System Microchamber Array Chip Fabrication Sample Loading with a Nanoliter Dispenser Multiplexed Detection of Different Target DNA on a Single Chip On‐chip Quantification of Amplified DNA On‐chip Cell‐free Protein Synthesis using A Picoliter Chamber Array Cell‐free Protein Synthesis Chip Fabrication Cell‐free Protein Synthesis using a Microchamber Array High‐throughput Single‐cell Analysis System using Pico‐liter Microarray Single‐cell Microarray Chip Fabrication Pico‐liter Microarray for Single‐cell Studies Single‐cell Microarray System for Analysis of Antigen‐specific Single B‐cells Conclusions Acknowledgments

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Pico/Nanoliter Chamber Array Chips for Single‐cell,DNAand Protein Analyses

Yamamura

Sathuluri

Tamiya

2003

Nanotechnologies for the Life Sciences

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Microsystems Technology and Biosensing

Sathuluri

Yamamura

Tamiya

Biosensing for the 21st Century

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This review addresses the recent developments in miniaturized microsystems or lab-on-a-chip devices for biosensing of different biomolecules: DNA, proteins, small molecules, and cells, especially at the single-molecule and single-cell level. In order to sense these biomolecules with sensitivity we have fabricated chip devices with respect to the biomolecule to be analyzed. The details of the fabrication are also dealt with in this review. We mainly developed microarray and microfluidic chip devices for DNA, protein, and cell analyses. In addition, we have introduced the porous anodic alumina layer chip with nanometer scale and gold nanoparticles for label-free sensing of DNA and protein interactions. We also describe the use of microarray and microfluidic chip devices for cell-based assays and single-cell analysis in drug discovery research.

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