A method to quantify several tyrosine kinase inhibitors in plasma by micellar liquid chromatography and validation according to the European Medicines Agency guidelines

Recent developments in the chromatographic bioanalysis of approved kinase inhibitor drugs in oncology

“…The main downside of light spectroscopy is the lower quantification limit, which in some cases is not sufficient to measure the in TDM commonly used trough-levels. This is true for most TKIs for which a relatively low trough level is required, such as afatinib [98], dasatinib [98,99,127], lapatinib, and sunitinib [105]. For ponatinib, and trametinib, the required trough levels are also expected to range below the detection limit for LV-UV (see Table 2).…”

“…Equilibrium dialysis is the most commonly used since non-specific adsorption of drugs to the device and membrane has less impact than other techniques, provided that an equilibrium is reached [103,104]. In TKI bioanalysis, ultracentrifugation is used for the determination of unbound imatinib [102] and erlotinib, imatinib, sunitinib, sorafenib, and lapatinib [105].…”

“…With the less selective UV-detection, better separation is often required. All eluting components should be base-line separated, requiring 20 to 35 minute runtimes [100,105,127] for up to 9 different drugs [127].…”

Antineoplastic drugs and their analysis: a state of the art review

“…Garrido-Cano et al developed an LC-UV method using a micellar mobile phase allowing direct injection of filtered plasma for the determination of 4 TK inhibitors. 464 A total analysis time of about 20 min and LOQ of 50 ng mL −1 were obtained. Finally, even if most of the TK inhibitors determination was performed with LC-UV and LC-MS, a LC-FD method was also published for the analysis of dasanitib in plasma with sensitivities close to those obtained in LC-UV (i.e., 50 ng mL −1 ).…”

Simultaneous determination of erlotinib and tamoxifen in rat plasma using UPLC–MS/MS: Application to pharmacokinetic interaction studies

“…They include HPLC-UV [15,16], HPLC-DAD [17], HPLC-MS/MS [18][19][20][21], and UPLC-MS/MS [22,23]. Also, TAM has been analyzed in biological matrices using numerous liquid chromatographic techniques, namely HPLCfluorimetry [24][25][26], HPLC-DAD [27], HPLC-UV [28], HPLC-MS/MS [26,[29][30][31][32], and UPLC-MS/MS [33][34][35][36].…”