A Methodological Study of the Enzymatic Determination of Glucose in Blood

Hjelm, M.; Verdier, C.-H. De

doi:10.3109/00365516309079764

Cited by 186 publications

(56 citation statements)

References 21 publications

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“…6 Serum insulin was measured by the Phadebas Insulin Test (Pharmacia, Uppsala, Sweden). 7 PAI-1 activity in plasma was measured with Spectolysea pL kits from Biopool AB (Umea Ê, Sweden), using polylysine as a stimulator.…”

Section: Methodsmentioning

confidence: 99%

Sagittal abdominal diameter compared with other anthropometric measurements in relation to cardiovascular risk

2000

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BACKGROUND: Abdominal adiposity has been described as an independent risk factor for coronary heart disease. Sagittal abdominal diameter has been found to be closely related to the amount of visceral adipose tissue. AIM: To compare the sagittal abdominal diameter with other anthropometric measures regarding their relationships to risk factors for coronary heart disease (CHD). DESIGN: A study of 885 men and women participating in a health survey. MEASUREMENTS: Sagittal abdominal diameter, body mass index (BMI), waist and hip circumferences, waist-to-hip ratio, serum concentrations of risk factors for CHD, blood pressure. RESULTS: In men the sagittal abdominal diameter showed stronger correlations to the CHD risk factors serum cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, glucose, insulin, apolipoprotein B (apoB), plasminogen activator inhibitor tissue-type plasminogen activator (t-PA) and lipid-corrected alpha tocopherol, and to systolic and diastolic blood pressures than the other anthropometric measurements. In women, compared with the other anthropometric measurements the sagittal abdominal diameter was more strongly correlated to serum cholesterol, LDL cholesterol, LDLaHDL (high-density lipoprotein), apo B and t-PA, and to systolic and diastolic blood pressure. The sagittal abdominal diameter showed a stronger correlation to`total risk' for cardiovascular disease ( 0.66 for men, 0.62 for women), than waist circumference ( 0.63 for men, 0.57 for women) and waist-to-hip ratio ( 0.61 for men and 0.48 for women; P`0.0001 for all correlations). This diameter was also more strongly correlated tò metabolic risk' ( 0.64 for men, 0.59 for women) than waist circumference ( 0.60 for men, 0.59 for women) and waist-to-hip ratio ( 0.58 for men, 0.52 for women)(P`0.0001 for all correlations). In a regression analysis including the anthropometric measurements and the risk values, the sagittal diameter was the strongest measure of cardiovascular risk in both men and women. CONCLUSIONS: Among both men and women in this study the sagittal abdominal diameter showed stronger correlations to cardiovascular risk and to other risk factors in the metabolic syndrome than other anthropometric variables such as waist circumference, waist-to hip ratio and BMI.

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Section: Methodsmentioning

confidence: 99%

Sagittal abdominal diameter compared with other anthropometric measurements in relation to cardiovascular risk

2000

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“…17 The serum insulin concentrations were measured with the Phadebas (Pharmacia, Uppsala, Sweden) test method based on a radioimmunological technique. 18 The TG and cholesterol (Choi) concentrations in serum and in the isolated lipoprotein fractions were determined by semiautomated methods in a Technicon Autoanalyzer II 19 by using a combination of preparative ultracentrifugation 20 and heparin-manganese precipitation 21 to separate VLDL, the low density lipoproteins (LDL), and HDL as earlier described.…”

Section: Blood Samplingmentioning

confidence: 99%

Serum lipoprotein and lipoprotein lipase in overweight, type II diabetics during and after supplemented fasting.

Vessby¹,

Selinus²,

Lithell³

1985

Arteriosclerosis

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“…There are obvious theoretical advantages in dispensing with the secondary peroxide-peroxidase chromogen step which is involved in most glucose oxidase methods for the measurement of glucose as it is this step which is most susceptible to interference and which is responsible for loss of specificity (Hjelm and de Verdier, 1963). Recently a number of publications (Kadish et al, 1968;Okuda and Okuda, 1969;Williams et al, 1970;Makin and Warren, 1970) have omitted this step by using electrometric techniques but each of these utilises a specially designed electrode and employs a manually operated discrete sampling system.…”

Section: Discussionmentioning

confidence: 99%

Continuous Flow Electrometric Glucose Determination

Wilde

Sewell

1972

Ann Clin Biochem

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A continuous flow system for the determination of glucose in blood or plasma using glucose oxidase is described. Oxygen uptake is measured by a Clarke electrode linked through an oxygen monitor and pH meter to a recorder. The omission of a peroxidase-chromogen step increases the specificity of the assay which gives very good correlation with the hexokinase-NADPH method.The system has a high sensitivity which can be adjusted by changing the oxygen-nitrogen ratio of the segmenting gas. It is useful when measuring 'true glucose' in low concentration and gives a linear response within the range 0 to 200 mg/loo ml. The electrode response time limits the rate of analysis to 30 samples per hour if adequate precision is to be maintained.The advantages of a closed continuous flow system over manual electrometric methods are discussed.

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A Methodological Study of the Enzymatic Determination of Glucose in Blood

Cited by 186 publications

References 21 publications

Sagittal abdominal diameter compared with other anthropometric measurements in relation to cardiovascular risk

Sagittal abdominal diameter compared with other anthropometric measurements in relation to cardiovascular risk

Serum lipoprotein and lipoprotein lipase in overweight, type II diabetics during and after supplemented fasting.

Continuous Flow Electrometric Glucose Determination

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