2018
DOI: 10.1038/s41598-018-23247-z
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A micro-CT-based method for quantitative brain lesion characterization and electrode localization

Abstract: Lesion verification and quantification is traditionally done via histological examination of sectioned brains, a time-consuming process that relies heavily on manual estimation. Such methods are particularly problematic in posterior cortical regions (e.g. visual cortex), where sectioning leads to significant damage and distortion of tissue. Even more challenging is the post hoc localization of micro-electrodes, which relies on the same techniques, suffers from similar drawbacks and requires even higher precisi… Show more

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Cited by 29 publications
(28 citation statements)
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“…To utilize micro-CT for structural imaging of the brain it is critical to add a substrate to provide 162 contrast to resolve different brain structures. In developing our protocol, our first goal was to 163 create as simple a procedure as possible, and one that does not require osmication, 164 dehydration of the tissue, nor imbedding in resin (Masís et al, 2018). We therefore developed a 165 protocol wherein we use eosin to provide contrast within the brain, and 166…”
Section: Results: 160mentioning
confidence: 99%
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“…To utilize micro-CT for structural imaging of the brain it is critical to add a substrate to provide 162 contrast to resolve different brain structures. In developing our protocol, our first goal was to 163 create as simple a procedure as possible, and one that does not require osmication, 164 dehydration of the tissue, nor imbedding in resin (Masís et al, 2018). We therefore developed a 165 protocol wherein we use eosin to provide contrast within the brain, and 166…”
Section: Results: 160mentioning
confidence: 99%
“…The CT protocol enables clear visualization of the lesioned area of the brain (Fig 3A). 196 Quantification of the lesion could be done by image segmentation, as was done with previous 197 micro-CT processing (Masís et al, 2018); however, image segmentation is laborious and prone 198 to subjectivity. Therefore, we sought a more automatable and objective approach for 199…”
Section: Lesion Quantification 191mentioning
confidence: 99%
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“…It has been recommended that we must select the contrast agent(s) effectively to enhance the contrast (Gignac et al, ; Metscher, , ; Smith et al, ). Recently, studies of micro‐CT observations for the brain of various animal taxa have been published (e.g., mammals, de Crespigny et al, ; Saito & Murase, ; birds, Gignac & Kley, ; Masis et al, ; fish, Kotrschal et al, ; insects, Smith et al, ; Steinhoff et al, ; cephalopods, Kerbl et al, ; Liu et al, ). Also, the contrast agents that are suitable for micro‐CT observation have been determined in some animals (Metscher, , ; Pauwels et al, ).…”
Section: Discussionmentioning
confidence: 99%
“…Micro-CT scan is a powerful technique capable of scanning a sample at various degrees using an x-ray to form 2D projections of the sample. Furthermore, a 3D volume can be generated using the collected 2D projections with the help of computer-based reconstruction techniques [7]. Therefore, the final output of micro-CT imaging technique is a high-resolution 3D volume.…”
Section: Molecular Micro-ct Scanmentioning
confidence: 99%