2009
DOI: 10.1039/b815475a
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A micro-ELISA system for the rapid and sensitive measurement of total and specific immunoglobulin E and clinical application to allergy diagnosis

Abstract: We have developed a novel, practical micro-ELISA system for sensitive and rapid allergy diagnosis. The enzymatic reactions occurred under stopped-flow conditions, resulting in both high precision and high sensitivity. A BSA-biotin-avidin linker was introduced for the immobilization of water-soluble allergens on polystyrene microbeads, enabling immobilization of allergens in sufficient density to provide high sensitivity. Evaluation of the system's performance showed a good detection limit (2 ng/mL) for total I… Show more

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Cited by 81 publications
(70 citation statements)
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“…Our group developed a microchip-based ELISA: micro-ELISA. 13,14 By introducing micro-beads with immobilized antibodies into a microchannel, very sensitive and fast ELISA was demonstrated. For the detection, thermal lens microscopy (TLM) was used, [15][16][17] which allowed for very sensitive detection based on enzymatically colorimetric reactions.…”
Section: Introductionmentioning
confidence: 99%
“…Our group developed a microchip-based ELISA: micro-ELISA. 13,14 By introducing micro-beads with immobilized antibodies into a microchannel, very sensitive and fast ELISA was demonstrated. For the detection, thermal lens microscopy (TLM) was used, [15][16][17] which allowed for very sensitive detection based on enzymatically colorimetric reactions.…”
Section: Introductionmentioning
confidence: 99%
“…2). 25 The microfluidic device was set on a Peltier temperature controller that controlled the reaction temperature. A 250-μL syringe pump was used for the infusion.…”
Section: Micro-padlock/rca Systemmentioning
confidence: 99%
“…A beads-based microfluidic ELISA chip (5)(6)(7) and a microfluidic device utilizing centrifugal force for liquid flow (8,9) have been reported. These systems effectively reduce assay time, although high throughput screening is not easily performed owing to the difficulty of parallel reactions of many reaction chambers.…”
Section: Introductionmentioning
confidence: 99%