A micro-QuEChERS method coupled to GC–MS for the quantification of pesticides in specific maternal and fetal tissues

Brandhonneur, Nolwenn; Mendes, Maïlys De Sousa; Lepvrier, Eléonore; Esseiva, E. Flejou; Chevanne, François; Corre, Pascal Le

doi:10.1016/j.jpba.2014.10.035

Cited by 26 publications

(20 citation statements)

References 29 publications

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“…The feasibility of this approach was based on preliminary work undertaken for a limited selection of pharmaceuticals (two) in biota, 17 bisphenol A in urine 33 and pesticides in tissues 41,32 . Typically, sample availability for environmental analyses are not a concern, however, for handling “hazardous” wastewater smaller sample sizes offer health and safety benefits by de‐risking the measurement of these materials.…”

Section: Resultsmentioning

confidence: 99%

An investigation of the utility of QuEChERS for extracting acid, base, neutral and amphiphilic species from example environmental and clinical matrices

Townsend

Keulen

Desbrow³

et al. 2020

Analytical Science Advances

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Accurate measurement of the composition of complex samples is key for the safety and efficacy of a range of products used in daily life, with sample preparation a critical step in this workflow. QuEChERS is one such method, however published protocols do not explicitly address acidic, basic, neutral, and amphiphilic species in a single protocol and often use extra steps or an alternative preparation to recover the breadth of chemical types. Our work addresses this need by investigating the use of QuEChERS for monitoring this wide range of chemistries within environmental solids and blood plasma, using a protocol that can accommodate both milliliter and microliter sample volumes. While published methods can require significant resource and time, our approach offers a reduction in preparation time (for environmental samples), with the "micro-QuEChERS" protocol offering a further reduction in cost. The analytical performance of these methods were assessed using reversed-phase LC-MS and showed good accuracy, precision, and sensitivity for the expected concentrations in the tested applications. Target analytes of variable lipophilicity/acidity were extracted and isolated from soil, with largely repeatable matrix effects < 15%RSD and recoveries of 39-100%. An initial "proof-of-concept" investigation using the "micro-QuEChERS" protocol showed reduced matrix enhancement (median value of 90%ME) for soil, and improved matrix effects and recovery (>65%) for blood plasma. This novel sample preparation method can therefore offer an improved approach with wider applicability providing "cleaner" extracts than other methods used for high-throughput clinical analysis.

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Section: Resultsmentioning

confidence: 99%

An investigation of the utility of QuEChERS for extracting acid, base, neutral and amphiphilic species from example environmental and clinical matrices

Townsend

Keulen

Desbrow³

et al. 2020

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…Extraction procedure was previously described (Brandhonneur et al, 2015). Briefly, blood samples (200 ul) and organ samples (weighting between 150 and 250 mg) were thawed, dehydrated and homogenized with MgSO 4 .…”

Section: Sample Preparationmentioning

confidence: 99%

“…We used Thermo Systems Instrument Trace GC and Trace DSQ2 for the simultaneous separation and quantification in samples of the chlorotriazines ATZ, SIM and PRO and their chlorinated metabolites DIA, DEA and DACT (Brandhonneur et al, 2015).…”

Section: Gc-ms Analytical Proceduresmentioning

confidence: 99%

Maternal and fetal blood pharmacokinetics and organ distribution of atrazine, propazine, simazine and their metabolites in pregnant rats after chronic oral administration

Brandhonneur

Hutin

Cordier

et al. 2019

Toxicological Sciences

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Environmental contamination by chlorotriazines has been evidenced in mother-child cohort suggesting more detailed risk assessment of these compounds in drinking water. Exposure of rodents to atrazine has been associated to alterations of endocrine and reproductive functions by disrupting neuroendocrine control at hypothalamus level. Perinatal exposure to low doses of atrazine has been associated to reproductive dysfunction, and to behavioral abnormalities in adult exposed during embryogenesis. The objectives of the current investigation were to (1) evaluate the influence of physico-chemical properties of chlorotriazines on tissue distribution in pregnant rats and in fetuses, (2) gain a better understanding of fetal distribution of chlorotriazines in specific tissues, particularly in brain. Serial blood samples were obtained from pregnant rats after administration of atrazine (ATZ), propazine (PRO) and simazine (SIM) via oral route at a dose of 10 mg/kg from day 15 to day 19. Maternal and fetal tissues were harvested at day 20, 24 hrs after the last dosing. The metabolic extraction ratio was estimated to 87% suggesting a significant first-pass effect explaining the low oral bioavailability. Blood exposure to parent compounds (ATZ, PRO and SIM) was negligible (lower than 5%) compared to metabolite exposure. The main metabolite exposure involved diamino-s-chlorotriazine (DACT), ranging from 60 to 90% depending on the molecules administered. A correlation between tissue-to-blood ratio and physico-chemical descriptors were observed for fat and mammary gland tissues but not for brain in adult rats. A more pronounced distribution in fetal brain was observed for ATZ and PRO, the two most lipophilic compounds.

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“…Recently, to compensate the rst problem to some extent, there is increasing trend of the development of miniaturized, more efficient, economical and green extraction methods that could signicantly reduce the toxic organic solvents and chemical consumption. [38][39][40] Particularly, QuEChERS sample preparation method got attentions for extraction of pesticides from complex food and other matrices because of its simplicity, miniaturization, inexpensiveness, efficiency, ruggedness and amenability to high throughput. However, it consists of multiple extraction and cleaning steps; despite that, it is unable to complete elimination of matrix interferences and risk of analyte contamination and loss are maximum during the multiple extraction, evaporation and reconstitution steps; especially, from complex food samples like honey, fruit and vegetables.…”

Section: Introductionmentioning

confidence: 99%

“…Besides these, QuEChERS method every time needs to modify according to the nature of matrix and analytes of interest. 19,38,39,[41][42][43][44] The matrix interferences and analyte loss problems can be resolved by making sample preparation method online with the 2D-IC system just like SPE methods aer making online proved more efficient, versatile, reproducible, selective, reduce labor, human error, costs, solvent and time. [45][46][47][48] Since last two decades, an online column-switching technique for heart-cutting is extensively utilized for complex samples clean-up and concentration to reduce matrix interferences.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive two-dimensional ion chromatography (2D-IC) coupled to a post-column photochemical fluorescence detection system for determination of neonicotinoids (imidacloprid and clothianidin) in food samples

et al. 2018

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A micro-QuEChERS method coupled to GC–MS for the quantification of pesticides in specific maternal and fetal tissues

Cited by 26 publications

References 29 publications

An investigation of the utility of QuEChERS for extracting acid, base, neutral and amphiphilic species from example environmental and clinical matrices

An investigation of the utility of QuEChERS for extracting acid, base, neutral and amphiphilic species from example environmental and clinical matrices

Maternal and fetal blood pharmacokinetics and organ distribution of atrazine, propazine, simazine and their metabolites in pregnant rats after chronic oral administration

Comprehensive two-dimensional ion chromatography (2D-IC) coupled to a post-column photochemical fluorescence detection system for determination of neonicotinoids (imidacloprid and clothianidin) in food samples

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