A micro thin layer chromatography technique for urinary and serum amino acids using fluorescamine as a staining reagent

A rapid semi-quantitative determination of phenylalanine, glycine and cystine from urine and phenylalanine from serum

“…Recently we described a micro technique for thin layer chromatography of amino acids from urine and serum using ftuorescamine as a staining reagent (Wang et al, 1983). We report here the extension of this study to a rapid semi-quantitative determination of urinary and/or serum amino acid concentrations.…”

“…All the material used is as described before (Wang et al, 1983). Chromatography and staining of amino acid were also as described (Wang et aL, 1983).…”

“…, 4, 2 folds; 10, undiluted serum; 1, standard amino acid mixture. Chromatographic procedures were as described in Wang et al (1983). Figure 1 shows the results by method I (sample dilution) applied to the estimation of concentration of urinary phenylalanine, glycine and cystine and serum phenylalanine.…”

“…One tenth of a microliter of this mixture was used for chromatography as described previously (Wang et al, 1983). Radioactivity was determined by (a) exposure of the plate to the ordinary K o d a k film overnight and by (b) elution of radioactivity from the thin layer plate (cellulose of the thin layer plate was scraped in 0.5 cm/fraction and eluted with 0.5 cc of water).…”

“…Five /A of undiluted radioactive [14C]phenylalanine (Wang et al, 1983) was added to 50/~l of serum. One tenth of a microliter of this mixture was used for chromatography as described previously (Wang et al, 1983).…”