A microarray approach to the identification of polyurethanes for the isolation of human skeletal progenitor cells and augmentation of skeletal cell growth

Tare, Rahul S.; Khan, Ferdous; Tourniaire, Guilhem; Morgan, Sara; Bradley, Mark; Oreffo, Richard O.C.

doi:10.1016/j.biomaterials.2008.10.038

Cited by 53 publications

(33 citation statements)

References 46 publications

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“…In this context, the physicochemical properties, such as hydrophobicity, roughness, formal charge and chemical composition are all known to play key roles in regulating cell-polymer reactivity [44][45][46][47][48]. Thus polymer materials lacking surface charge are known to mediate their effects on cell attachment by physiosorption of extracellular matrix (ECM) proteins [49].…”

Section: Discussionmentioning

confidence: 99%

Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

et al. 2010

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“…This was demonstrated with osteoprogenic cells, whereupon polymer microarrays were used to identify a material from a library of polyurethanes that could exclusively bind STRO-1+ cells, derived from a human bone marrow mononuclear cell population. This material was used to isolate these cells from other immature osteoblasts like MG-63 cells, STRO-1+ human fetal skeletal cells and differentiated osteoblast-like SoOs cells, demonstrating the selectivity of the identified material [85].…”

Section: Assessment Of Biological Response To Microarraysmentioning

confidence: 96%

High throughput methods applied in biomaterial development and discovery

et al. 2010

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The high throughput discovery of new materials can be achieved by rapidly screening many different materials synthesised by a combinatorial approach to identify the optimal material that fulfils a particular biomedical application. Here we review the literature in this area and conclude that for polymers, this process is best achieved in a microarray format, which enable thousands of cell-material interactions to be monitored on a single chip. Polymer microarrays can be formed by printing pre-synthesised polymers or by printing monomers onto the chip where on-slide polymerisation is initiated.The surface properties of the material can be analysed and correlated to the biological performance using high throughput surface analysis, including time-of-flight secondary ion mass spectrometry (ToF-SIMS), Xray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements. This approach enables the surface properties responsible for the success of a material to be understood, which in turn provides the foundations of future material design. The high throughput discovery of materials using polymer microarrays has been explored for many cell-based applications including the isolation of specific 2 cells from heterogeneous populations, the attachment and differentiation of stem cells and the controlled transfection of cells.Further development of polymerisation techniques and high throughput biological assays amenable to the polymer microarray format will broaden the combinatorial space and biological phenomenon that polymer microarrays can explore, and increase their efficacy. This will, in turn, result in the discovery of optimised polymeric materials for many biomaterial applications.

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“…Recent developments in high-throughput screening of combinatorial libraries of materials and soluble factors offer a cost-effective tool to identify conditions and materials that support self-renewal and differentiation of stem cells [21–28]. In this study, we modified our array-based high-throughput approach[29, 30] for systematic investigation of the effects of polymeric biomaterials on hPSC self-renewal.…”

Section: Introductionmentioning

confidence: 99%

Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces

et al. 2010

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Realization of the full potential of human pluripotent stem cells (hPSCs) in regenerative medicine requires the development of well-defined culture conditions for their long-term growth and directed differentiation. Current practices for maintaining hPSCs generally utilize empirically determined combinations of feeder cells and other animal-based products, which are expensive, difficult to isolate, subject to batch-to-batch variations, and unsuitable for cell-based therapies. Using a high-throughput screening approach, we identified several polymers that can support self-renewal of hPSCs. While most of these polymers provide support for only a short period of time, we identified a synthetic polymer poly(methyl vinyl ether-alt-maleic anhydride) (PMVE-alt-MA) that supported attachment, proliferation and self-renewal of HUES1, HUES9, and iPSCs over five passages. The hPSCs cultured on PMVE-alt-MA maintained their characteristic morphology, expressed high levels of markers of pluripotency, and retained a normal karyotype. Such cost-effective, polymer-based matrices that support long-term self-renewal and proliferation of hPSCs will not only help to accelerate the translational perspectives of hPSCs, but also provide a platform to elucidate the underlying molecular mechanisms that regulate stem cell proliferation and differentiation.

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A microarray approach to the identification of polyurethanes for the isolation of human skeletal progenitor cells and augmentation of skeletal cell growth

Cited by 53 publications

References 46 publications

Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

High throughput methods applied in biomaterial development and discovery

Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces

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