A microbiological study in relation to the presence of caries and calculus

Dahlén, Gunnar; Konradsson, Karolina; Eriksson, Sophia; Teanpaisan, Rawee; Piwat, Supatcharin; Carlén, Anette

doi:10.3109/00016351003745514

Cited by 18 publications

(15 citation statements)

References 34 publications

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“…The majority of studies using the checkerboard DNA-DNA hybridisation technique have focused on studying plaque bacterial ecology in relation to periodontal disease [14-17]. These studies revealed a directly proportional relationship between the bacterial count and periodontal pocket depth.…”

Section: Resultsmentioning

confidence: 99%

Supragingival plaque microbial analysis in reflection to caries experience

et al. 2013

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BackgroundDental caries develops as a result of the metabolism of carbohydrates by cariogenic bacteria present in a complex biofilm. The present study aimed to examine if bacteria in pooled supragingival plaque samples quantified using a “checkerboard DNA-DNA hybridization” based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous investigation.MethodsA total of 86 mothers and their children aged 4–6 years and 12–16 years old participated. Caries experience (DMFT/dmft; Decayed, Missing and Filled Teeth for permanent and primary teeth) was registered clinically and radiographically. Caries was recorded at the D3 level (caries into dentine). The D/d component was divided into three categories. A pooled supragingival plaque sample per participant was obtained from posterior approximal sites. Analyses of 15 bacterial species were performed using the checkerboard DNA-DNA hybridisation technique.ResultsNo significant relationships were found between the bacterial scores and DMFT/dmft nor D/d groups.ConclusionsUnlike the saliva samples and the chair-side method, interproximal pooled plaque samples analysed using the “checkerboard DNA-DNA hybridization technique” did not reveal any significant relations between the bacterial counts and the caries experience.

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Section: Resultsmentioning

confidence: 99%

Supragingival plaque microbial analysis in reflection to caries experience

et al. 2013

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“…F. alocis , a fastidious, obligate, anaerobic rod‐shaped bacterium, has been repeatedly identified in periodontal lesions 25,26 and was considered to be an excellent marker organism for periodontal disease 27,28 . Additionally, F. alocis was previously found to be associated with caries plaque 29 and endodontic infection, including periapical lesions 30,31 . Studies showed that F. alocis predominantly colonized the apical and middle third of the carriers, preferably settled on the side of the carrier facing the soft tissues, 32 and has the potential to contribute to periodontal tissue breakdown 27 .…”

Section: Discussionmentioning

confidence: 99%

Bacteria Community Study of Combined Periodontal‐Endodontic Lesions Using Denaturing Gradient Gel Electrophoresis and Sequencing Analysis

Guan

Sun

et al. 2014

Journal of Periodontology

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“…However, through molecular approaches it is becoming increasingly apparent that the presence of F. alocis is indicative of a number of oral diseases including caries, endodontic infections and periodontal disease. Filifactor alocis is weakly glycolytic, and children with caries have been shown to have elevated levels of F. alocis in plaque (Dahlen et al. , 2010).…”

Section: Introductionmentioning

confidence: 99%

Filifactor alocis interactions with gingival epithelial cells

Moffatt

Whitmore

Griffen

et al. 2011

Molecular Oral Microbiology

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An association between the gram-positive anaerobe Filifactor alocis and periodontal disease has recently emerged; however, possible pathogenic mechanisms have not been investigated. In this study we examined the responses of primary cultures of gingival epithelial cells (GECs) to infection with F. alocis. Secretion of the proinflammatory cytokines IL-1β, IL-6 and TNF-α from GECs was stimulated by F. alocis infection. F. alocis also induced apoptosis in GECs through pathways that involved caspase-3 but not caspase-9. Apoptosis was coincident with inhibition of MEK (MAPK kinase) activation. These results show that F. alocis has characteristics in common with established periodontal pathogens and has the potential to contribute to periodontal tissue destruction.

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A microbiological study in relation to the presence of caries and calculus

Cited by 18 publications

References 34 publications

Supragingival plaque microbial analysis in reflection to caries experience

Supragingival plaque microbial analysis in reflection to caries experience

Bacteria Community Study of Combined Periodontal‐Endodontic Lesions Using Denaturing Gradient Gel Electrophoresis and Sequencing Analysis

Filifactor alocis interactions with gingival epithelial cells

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