A microfluidic biosensor for rapid simultaneous detection of waterborne pathogens

Muhsin, Sura A.; Al-Amidie, Muthana; Shen, Zhenyu; Mlaji, Zahar; Liu, Jiayu; Abdullah, Amjed; El-Dweik, Majed; Zhang, Shuping; Almasri, Mahmoud

doi:10.1016/j.bios.2022.113993

Cited by 36 publications

(12 citation statements)

References 15 publications

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“…S.C. Terry reported the first lab-on-a-chip (LOC) analysis system in 1979, which was investigated for gas chromatography applications [37]. Since then, microfluidic devices have been investigated for a variety of applications, including biosensors [38], separation [39], analysis [40], drug delivery [41,42], optoelectronics [43], cell manipulation [44], and chemical synthesis [45,46]. There has been much advancement in surface chemistry, which has enabled the development of smart surfaces and devices for various applications [47,48].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

et al. 2022

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CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages.

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Section: Introductionmentioning

confidence: 99%

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

et al. 2022

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“…Using pDEP enrichment, the bacteria are focused on the edges of interdigitated electrodes functionalized with antibodies, bacterial capture was found to be 3–5 fold higher than for immunocapture without DEP enrichment. A recent example is the work by Muhsin et al [ 88 ] who focused bacteria onto an antibody functionalized IDE array, thus achieving selective detection of living legionella cells in water samples in concentration of 3 cells/mL. Similar result was also reported for capacity sensors based on single wall carbon nanotubes (SWCN) [ 89 ].…”

Section: Current Trends In Biosensors Assisted By Dielectrophoresismentioning

confidence: 62%

Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?

2022

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The performance of receptor-based biosensors is often limited by either diffusion of the analyte causing unreasonable long assay times or a lack of specificity limiting the sensitivity due to the noise of nonspecific binding. Alternating current (AC) electrokinetics and its effect on biosensing is an increasing field of research dedicated to address this issue and can improve mass transfer of the analyte by electrothermal effects, electroosmosis, or dielectrophoresis (DEP). Accordingly, several works have shown improved sensitivity and lowered assay times by order of magnitude thanks to the improved mass transfer with these techniques. To realize high sensitivity in real samples with realistic sample matrix avoiding nonspecific binding is critical and the improved mass transfer should ideally be specific to the target analyte. In this paper we cover recent approaches to combine biosensors with DEP, which is the AC kinetic approach with the highest selectivity. We conclude that while associated with many challenges, for several applications the approach could be beneficial, especially if more work is dedicated to minimizing nonspecific bindings, for which DEP offers interesting perspectives.

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“…Thus, exploring on-site ultrafast, highly sensitive, and specific detection sensors to monitor waterborne pathogens in early stages is essential to improve public health all over the world. Muhsin et al developed a microfluidic impedance biosensor for the simultaneous analysis of E. coli O157:H7, Salmonella, and Legionella in tap water and wastewater samples [114].…”

Section: Waterborne Pathogenic Bacteriamentioning

confidence: 99%

“…Ingestion of water contaminated by pathogenic bacteria results in various infectious diseases that affect public health in both developing and developed countries [93, 114, 115]. It is reported that 7.15 million people are infected with waterborne pathogens every year and the death cases reach 6630 with economic loss surpassing $3.3 billion directed to the healthcare system [116].…”

Section: Microchip Electrophoresis For Analysis Of Pathogensmentioning

confidence: 99%

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Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses

et al. 2022

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Life‐threatening diseases, such as hepatitis B, pneumonia, tuberculosis, and COVID‐19, are widespread due to pathogenic bacteria and viruses. Therefore, the development of highly sensitive, rapid, portable, cost‐effective, and selective methods for the analysis of such microorganisms is a great challenge. Microchip electrophoresis (ME) has been widely used in recent years for the analysis of bacterial and viral pathogens in biological and environmental samples owing to its portability, simplicity, cost‐effectiveness, and rapid analysis. However, microbial enrichment and purification are critical steps for accurate and sensitive analysis of pathogenic bacteria and viruses in complex matrices. Therefore, we first discussed the advances in the sample preparation technologies associated with the accurate analysis of such microorganisms, especially the on‐chip microfluidic‐based sample preparations such as dielectrophoresis and microfluidic membrane filtration. Thereafter, we focused on the recent advances in the lab‐on‐a‐chip electrophoretic analysis of pathogenic bacteria and viruses in different complex matrices. As the microbial analysis is mainly based on the analysis of nucleic acid of the microorganism, the integration of nucleic acid‐based amplification techniques such as polymerase chain reaction (PCR), quantitative PCR, and multiplex PCR with ME will result in an accurate and sensitive analysis of microbial pathogens. Such analyses are very important for the point‐of‐care diagnosis of various infectious diseases.

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A microfluidic biosensor for rapid simultaneous detection of waterborne pathogens

Cited by 36 publications

References 15 publications

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?

Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses

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