A Microfluidic Chip and Dark-Field Imaging System for Size Measurement of Metal Wear Particles in Oil

Haiden, Christoph; Wopelka, Thomas; Jech, Martin; Keplinger, Franz; Vellekoop, Michael J.

doi:10.1109/jsen.2015.2501355

Cited by 35 publications

(15 citation statements)

References 40 publications

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“…As this work was a proof of principle, a lab microscope was used to verify the feasibility of the system. Portable solutions have already been presented in [ 30 , 31 ] and it is the aim of future work to miniaturize the system for fluorescence single particle tracking in order for it to be transportable for in-field measurements.…”

Section: Discussionmentioning

confidence: 99%

“…A MATLAB script (2011b, MathWorks, Natick, MU, USA) utilizing 2D particle tracking (Crocker–Grier algorithm [ 28 ]) is used to link the exact position (centroids) of objects appearing over subsequent frames [ 29 , 30 ]. Based on this approach, a single frame i ( i = 1, …, N ) is processed to detect multiple bright spots (representing particles) over a dark background (here the variability in pixel intensity is used to ‘find’ a particle) (see Figure 3 b).…”

Section: Design and Methodsmentioning

confidence: 99%

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Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples

Malec

Haiden

Giouroudi

2018

Sensors

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Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illness and even death. Rapid and multiplexed measurement of such waterborne pathogens is vital and the challenge is to instantly detect in these liquid samples different types of pathogens with high sensitivity and specificity. In this work, we propose a biosensing system in which the bacteria are labelled with streptavidin coated magnetic markers (MPs—magnetic particles) forming compounds (MLBs—magnetically labelled bacteria). Video microscopy in combination with a particle tracking software are used for their detection and quantification. When the liquid containing the MLBs is introduced into the developed, microfluidic platform, the MLBs are accelerated towards the outlet by means of a magnetic field gradient generated by integrated microconductors, which are sequentially switched ON and OFF by a microcontroller. The velocities of the MLBs and that of reference MPs, suspended in the same liquid in a parallel reference microfluidic channel, are calculated and compared in real time by a digital camera mounted on a conventional optical microscope in combination with a particle trajectory tracking software. The MLBs will be slower than the reference MPs due to the enhanced Stokes’ drag force exerted on them, resulting from their greater volume and altered hydrodynamic shape. The results of the investigation showed that the parameters obtained from this method emerged as reliable predictors for E. coli concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Design and Methodsmentioning

confidence: 99%

Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples

Malec

Haiden

Giouroudi

2018

Sensors

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“…Many authors use optical setups for microcantilevers. However, an optical output has several disadvantages during operation in water when the refractive index of water changes [30,31].…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive detection of cadmium ions using a microcantilever-based piezoresistive sensor for groundwater

Rotake

Darji

Kale

2020

Beilstein J. Nanotechnol.

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This paper proposes the selective and ultrasensitive detection of Cd(II) ions using a cysteamine-functionalized microcantilever-based sensor with cross-linked ᴅʟ-glyceraldehyde (DL-GC). The detection time for various laboratory-based techniques is generally 12–24 hours. The experiments were performed to create self-assembled monolayers (SAMs) of cysteamine cross-linked with ᴅʟ-glyceraldehyde on the microcantilever surface to selectively capture the targeted Cd(II). The proposed portable microfluidic platform is able to achieve the detection in 20–23 min with a limit of detection (LOD) of 0.56 ng (2.78 pM), which perfectly describes its excellent performance over other reported techniques. Many researchers used nanoparticle-based sensors for the detection of heavy metal ions, but daily increasing usage and commercialization of nanoparticles are rapidly expanding their deleterious effect on human health and the environment. The proposed technique uses a blend of thin-film and microcantilever (micro-electromechanical systems) technology, which mitigate the disadvantages of the nanoparticle approaches, for the selective detection of Cd(II) with a LOD below the WHO limit of 3 μg/L.

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“…Unlike the absorption process, the scattering process is directional. Knowing the directional scattering properties is highly informative for identifying the size and shape of suspended particles in water [1]- [7].…”

Section: Introductionmentioning

confidence: 99%

Measuring the Three-Dimensional Volume Scattering Functions of Microsphere Suspension: Design and Laboratory Experiments

Wang

Yang

et al. 2019

IEEE Photonics J.

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The measurement of the volume scattering function (VSF) of suspended particles is an ongoing research topic since it has the potential to provide a rapid, nondestructive means of analyzing hydrosols. Despite the success of scattering theory, the measurement of multidirectional scattering intensity distributions over a wide, continuous range of scattering angles and multiple scattering planes, which is described by a three-dimensional VSF (3D VSF), has proven to be difficult. To measure the 3D VSF of submicron particles suspended in water, a measurement approach is proposed, built and tested with several standard submicron particles with diameters ranging from 60 nm to 1 μm. The experimental results indicate that the proposed setup can obtain an accurate 3D VSF for scattering angles from 21°to 160°and azimuthal angles from 1°to 179°. Comparisons between the experiment and theory show a high level of consistency for particles with diameters less than 200 nm. The variation in the VSF in response to the polarization direction of the incident light is also discussed. Both the experiment and theory show the potential of the device to detect the 3D VSF of marine submicron biological particles.

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A Microfluidic Chip and Dark-Field Imaging System for Size Measurement of Metal Wear Particles in Oil

Cited by 35 publications

References 40 publications

Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples

Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples

Ultrasensitive detection of cadmium ions using a microcantilever-based piezoresistive sensor for groundwater

Measuring the Three-Dimensional Volume Scattering Functions of Microsphere Suspension: Design and Laboratory Experiments

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