A Microfluidic Chip Based on Localized Surface Plasmon Resonance for Real-Time Monitoring of Antigen–Antibody Reactions

Abstract

Localized surface plasmon resonance (LSPR) connecting to noble metal nanoparticles is an important issue for many analytical and biological applications. Therefore, the development of microfluidic LSPR chip that allows studying biomolecular interactions becomes an essential requirement for micro total analysis systems (mTAS) integration. However, miniaturized process of the conventional surface plasmon resonance system has been faced with some limitations, especially with the usage of Kretschmann configuration in total internal reflection mode. In this study, we have tried to solve this problem by proposing a novel microfluidic LSPR chip operated with a simple collinear optical system. The poly(dimethylsiloxane) (PDMS) based microfluidic chip was fabricated by soft-lithography technique and enables to interrogate specific insulin and anti-insulin antibody reaction in real-time after immobilizing antibody on its surface. Moreover, the sensing ability of microfluidic LSPR chip was also evaluated with various glucose concentrations. The kinetic constant of insulin and anti-insulin antibody was determined and the detection limit of 100 ng/mL insulin was archived.

Localized Surface Plasmon Resonance as a Biosensing Platform for Developing Countries

“…Insulin is one of the most important indicators for diabetes diagnosis, a disease where 80% of related deaths occur in developing countries [ 40 ]. A novel polydimethylsiloxane (PDMS) microfluidic LSPR chip has been proposed to interrogate antibody-antigen reactions in real time with the chip later used to detect insulin levels in the real-time monitoring of insulin and anti-insulin antibody immunoreactions with a 100 ng/mL detection limit [ 41 ].…”

Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

“…At the same time, a large amount of sample volume is needed, which has a great impact on the saving of precious samples especially in clinical applications [16][17][18]. Aside from the technologies mentioned above, the localized surface plasmon resonance (LSPR) is another widely used method for fast, label-free and real-time monitoring of biomolecule binding events [19][20][21][22]. The LSPR is a plasmonic phenomenon that arises around nanoscale structures or nanoparticles of noble metals when light is illuminated onto a nanoscale-featured sensing surface [21,[23][24][25].…”

New trends in instrumental design for surface plasmon resonance-based biosensors

“…Working with droplets rather than direct flow has the advantage of improving the accuracy of kinetic parameters measurements in the mass transport-limited regime. In addition to conventional SPR, microfluidic microsystems were also applied to localized SPR (Hiep et al 2008; Huang et al 2009). …”