New trends in instrumental design for surface plasmon resonance-based biosensors

Abbas, Abdennour; Linman, Matthew J.; Cheng, Quan

doi:10.1016/j.bios.2010.09.030

Cited by 273 publications

(154 citation statements)

References 121 publications

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“…In our SPR system, the sensitivity can be reached up to 30 pg/cm 2 (0.3 RU) in case of protein adsorption. In order to add high selectivity, biochemical recognition elements, such as antibodies, enzymes, proteins, DNA and cells are immobilized on the solid surface of the SPR sensor [7][8][9][10]. Biochemical recognition elements can be immobilized by physical adsorption [11], by embedding in polymers or membranes [12,13], by trapping in solgels [14,15], and by using functionalized alkanethiol or functionalized alkylsilane self-assembled monolayer (SAM) [16,17].…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…200 SPR is a collective oscillation of free charges present at the interface of two media (metal-dielectric), with permittivities of opposite sign. 201 This method is based on measuring the refractive index of very thin layers of material adsorbed on a metal surface. At certain conditions, surface plasmons on a metallic film can be excited by photons, transforming a photon into a surface plasmon depending on the refractive index of the adsorbate.…”

Section: Spr Biosensorsmentioning

confidence: 99%

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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“…We initially consider the attenuated total reflection (ATR) method with a Kretschmann configuration (Fig.1) [5] as this forms the basis of the theoretical model for the fiber SPR-based sensor proposed in this paper. As shown in Fig.1, the structure comprises a coupling prism with a high refractive index (RI), a metal layer and a sensing sample.…”

Section: Theoretical Formalismmentioning

confidence: 99%

“…Traditional SPR sensors are based on the so-called Kretschmann configuration, where a thin metal film is deposited on the base of a coupling prism [4]. For such prism-based SPR sensors, Homola provides a comprehensive overview of developments in the past 20 years and numerous designs for novel SPR biosensors [5]. However, a prism-based SPR configuration has a number of disadvantages, such as bulky size and complex operation and in addition real time/remote sensing is difficult to implement.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity enhancement for a multimode fiber sensor with an axisymmetric metal grating layer

Farrell

Semenova

et al. 2014

Photonics and Nanostructures - Fundamentals and Applications

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This paper proposes a novel design for a surface plasmon resonance (SPR) fiber sensor with an axisymmetric sub-wavelength metal grating layer. The relationship between the sensor performance (the sensitivity S and the quality factor Q of the SPR dip) and the characteristic parameters are investigated. Numerical simulation results show that the proposed sensor can achieve a maximum sensitivity of 13000nm/RIU (refractive index unit) for a refractive index range from 1.3 to 1.4.

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New trends in instrumental design for surface plasmon resonance-based biosensors

Cited by 273 publications

References 121 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Nanoscale virus biosensors: state of the art

Sensitivity enhancement for a multimode fiber sensor with an axisymmetric metal grating layer

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