A microfluidic device for noninvasive cell electrical stimulation and extracellular field potential analysis

Ni, Liwei; Kc, Pawan; Mulvany, Emily; Zhang, Ge; Zhe, Jiang

doi:10.1007/s10544-019-0364-2

Cited by 11 publications

(8 citation statements)

References 37 publications

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“…These limitations overcame in microfluidic chip EStim chambers [129][130][131][132]. Microfluidic EStim chambers consist of (1) an inlet for loading cells, (2) a main fluidic channel, (3) a constriction microchannel/microchip, (4) a pair of stimulation electrodes for applying electrical stimulation and reference electrodes for measuring extracellular field potential simultaneously, and (5) an outlet reservoir for collection of cells after EStim [133]. To use this chamber, cells are first loaded through the inlet, then, by controlling the driving pressure of the flow and using a constriction channel, cells are trapped on the surface of measurement electrodes, where they are exposed to EStim.…”

Section: Microfluidic Chip Estim Chambersmentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

162

148

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Electrical stimulation (EStim) has been shown to promote bone healing and regeneration both in animal experiments and clinical treatments. Therefore, incorporating EStim into promising new bone tissue engineering (BTE) therapies is a logical next step. The goal of current BTE research is to develop combinations of cells, scaffolds, and chemical and physical stimuli that optimize treatment outcomes. Recent studies demonstrating EStim's positive osteogenic effects at the cellular and molecular level provide intriguing clues to the underlying mechanisms by which it promotes bone healing. In this review, we discuss results of recent in vitro and in vivo research focused on using EStim to promote bone healing and regeneration and consider possible strategies for its application to improve outcomes in BTE treatments. Technical aspects of exposing cells and tissues to EStim in in vitro and in vivo model systems are also discussed.

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Section: Microfluidic Chip Estim Chambersmentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

162

148

View full text Add to dashboard Cite

show abstract

“…Other interesting microfluidic platform was developed in order to quickly apply versatile ES signals to cells suspended in microfluidic channels and measure extracellular field potential simultaneously. [ 285 ] The system was able not just to noninvasively distinguish electrically excitable cells, such as cardiomyocytes cells, from electrically nonexcitable cells, such as human umbilical vein endothelial cells, but also to detect viable cells in cardiac tissue. The results demonstrate the potential of this tool to optimize the electric stimulation conditions to facilitate the functional engineered cardiac tissue development.…”

Section: Physically Active Bioreactors—main Typesmentioning

confidence: 99%

Physically Active Bioreactors for Tissue Engineering Applications

et al. 2020

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Tissue engineering (TE) is a dynamic and growing scientific field that merges the knowledge of different areas such as biology, physics, medicine, and engineering. [1] The TE discipline was first coined at a National Science Foundation sponsored meeting in 1987, thus originating a field that employs life sciences and engineering with the purpose of developing biologic or synthetic supports to restore, keep, or enhance tissue functions or damaged organs. [2] The classical TE approach has been mainly focusing on associating cells with a supporting matrix, also called biomaterials or scaffolds, that essentially acts as a passive template for tissue formation in vitro by allowing cells to adhere, migrate, differentiate, and produce tissue. Usually, the cells are seeded onto the scaffolds and, occasionally, growth factors are also added. The combination of cells, growth factors, and scaffold is often referred as the TE triad. [3] Nevertheless, novel approaches in TE that include the application of a biophysical stimuli to the scaffolds through the use of a bioreactor are emerging. [4] Tissue engineering (TE) is a strongly expanding research area. TE approaches require biocompatible scaffolds, cells, and different applied stimuli, which altogether mimic the natural tissue microenvironment. Also, the extracellular matrix serves as a structural base for cells and as a source of growth factors and biophysical cues. The 3D characteristics of the microenvironment is one of the most recognized key factors for obtaining specific cell responses in vivo, being the physical cues increasingly investigated. Supporting those advances is the progress of smart and multifunctional materials design, whose properties improve the cell behavior control through the possibility of providing specific chemical and physical stimuli to the cellular environment. In this sense, a varying set of bioreactors that properly stimulate those materials and cells in vitro, creating an appropriate biomimetic microenvironment, is developed to obtain active bioreactors. This review provides a comprehensive overview on the important microenvironments of different cells and tissues, the smart materials type used for providing such microenvironments and the specific bioreactor technologies that allow subjecting the cells/ tissues to the required biomimetic biochemical and biophysical cues. Further, it is shown that microfluidic bioreactors represent a growing and interesting field that hold great promise for achieving suitable TE strategies.

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“…Such in vitro devices include, but are not limited to, devices for measuring the electrical impedance of cells to evaluate specific biological activities (e.g., proliferation), 4 devices for inducing specific cell responses by means of low voltage electrical stimulation (e.g., cell migration, differentiation), 3,5,6 and devices for stimulating and/or recording electrical activities of cells using multi-electrode arrays (MEAs) and multipurpose setups (e.g., lab-on-achip). [7][8][9] In both in vivo and in vitro electrical stimulation and recording devices, electrodes are the key components responsible for communicating electrical signals with the extracellular fluid (ECF). For biologically safe and effective stimulation and recording, electrodes should provide several physical, electrochemical, mechanical, and biological properties that might not be found in a single material.…”

Section: Introductionmentioning

confidence: 99%