A microfluidic device for tissue biopsy culture and interrogation

Abstract

This communication reports the development of a microfluidic device capable of maintaining the long-term culture of viable tissue biopsies. Tissue-based models will enable evaluation of cell-cell and cellmatrix interactions within multi-cellular systems. The device demonstrated is a prototype, fabricated with the capacity to receive biopsy samples up to 2 mm 3 , from various tissue sources. Presently, this system has been tested with human colorectal tissue biopsies, for periods in excess of 3 days. The response of normal colorectal tissue and neoplastic biopsies to hypoxia was assayed by the release of vascular endothelial growth factor (VEGF) into the media, which was measured off-chip. As anticipated, the hypoxia induced a greater VEGF response in the tumour biopsies than the nonmalignant tissue.

A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow

“…The initial high levels of LDH release are thought to be due to unavoidable damage caused by the handling of the tissue from the resection and then preparation for insertion into the device. [23][24][25][26]30,31 To quantify the proportion of viable cells that remained in the tissue throughout the duration of the experiment, a portion of the tissue taken from patients was immediately put into 10% v/v lysis solution for 24 h, and the total LDH release was measured. Similarly, tissue which had been in the device for 24 or 72 h was also treated in this way, and the total amounts released were quantified (Fig.…”

Microfluidic 3D Cell Culture: Potential Application For Tissue-Based Bioassays

“…Since glass is impermeable to oxygen, this property has been used to create hypoxic environment for oxygen related cellular studies [50]. For instance, Webster et al reported a glass microfluidic device for tissue biopsy culture [50].…”

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

“…7,8 To date, a number of biomedical application, such as cell-based investigation, in particular, cell cultivation and proliferation has been shown to great success relied on microfluidic chip. 9,10 Apart from cells, [11][12][13] embryos, [14][15][16][17][18] nematodes, [19][20][21][22] and tissues, 23,24 all have realized on-chip cultivation or manipulation. However, the microscale channels fail to easily handle the multicellular organism of large size, such as zebrafish embryos, which might obscure their promise of application in compound-related toxicity and teratogenicity assay.…”