A microfluidic immunosensor for automatic detection of carcinoembryonic antigen based on immunomagnetic separation and droplet arrays

Abstract

Using immunomagnetic separation technology to enrich the target in the sample, combined with solid-phase surface modification technology to complete the quantitative detection of the sample concentration, enabling a more simplified detection process.

Advanced manufacturing of nanoparticle formulations of drugs and biologics using microfluidics

“…164 The separation and purification of nano-drugs and biologics, such as liposomes, viruses, DNA nano-balls, and extracellular vesicles (EVs), are essential prerequisites for their biomedical and clinical applications. [165][166][167][168] Both passive and active techniques including acoustofluidics, [169][170][171] hydrodynamics, [172][173][174] and dielectrophoresis (DEP) [175][176][177][178][179] have been employed for the separation and purification of these substances.…”

“…Furthermore, surface acoustic waves (SAWs) are generated by a transducer called an interdigital transducer (IDT) and particle movements are affected by SAWs, as shown in Fig.8D 164. The separation and purification of nano-drugs and biologics, such as liposomes, viruses, DNA nano-balls, and extracellular vesicles (EVs), are essential prerequisites for their biomedical and clinical applications [165][166][167][168]. Both passive and active techniques including acoustofluidics,[169][170][171] hydrodynamics,[172][173][174] and dielectrophoresis (DEP)[175][176][177][178][179] have been employed for the separation and purification of these substances.Acoustofluidic technologies make use of acoustic waves to precisely control fluids and particles immersed in fluids, which enables contact-free and biocompatible separation of NPs.…”

Biosensor-Based Nanodiagnosis of Carcinoembryonic Antigen (CEA): an Approach to Classification and Precise Detection of Cancer Biomarker

“…5 Representation of the process of constructing a self-supporting electrochemical biosensor based on PPy composite film, adapted from Ref (43) immunomagnetic separation. The developed platform has the benefits of automation, cost-effectiveness, high throughput, low sample consumption, and encouraging applications in biochemistry [53]. ELC immunosensor for rapid determination of CEA using luminol-coated AgNPs was fabricated.…”

A digital microfluidic device integrated with electrochemical sensor and 3D matrix for detecting soluble PD-L1

“…As an alternative, immunosensors have been gaining attention as they can be easily integrated with microfluidic technologies to create a point-of-care (POC) tool ( Hu et al, 2023 ; Li and Lillehoj ). In these sensors, antibodies conjugate with target analytes to produce specific changes in output signal.…”