A microfluidic platform for real-time and <i>in situ</i> monitoring of virus infection process

Xu, Na; Zhang, Zhenfeng; Wang, Li; Gao, Bo; Pang, Dai‐Wen; Wang, Hanzhong; Zhang, Zhiling

doi:10.1063/1.4756793

Cited by 21 publications

(17 citation statements)

References 38 publications

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“…Microfluidic technologies have also been applied to investigate viral replication. Several studies have used microfluidic chips, coupled with fluorescent imaging to quantify viral replication in a variety of cell types [32,33]. That microfluidic techniques have proved highly compatible with live imaging experiments further validates their usefulness for this purpose.…”

Section: Microfluidic Platforms For the Study Of Viral Cell Biology Amentioning

confidence: 88%

Microfluidics: an Untapped Resource in Viral Diagnostics and Viral Cell Biology

Simpson

Lee

et al. 2018

Curr Clin Micro Rpt

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Purpose of Review Microfluidic platforms have become valuable tools in a wide variety of research environments. With the ability to allow detailed examination of an array of cell biological processes, their use in the field of virology is becoming progressively more common. This review will discuss the potential applications of microfluidics in viral cell biology and explore the potential of these techniques to alter the way in which we study the biology of infection. Recent Findings In recent years, scientists have utilised microfluidic platforms for detailed study of the viral life cycle. Microfluidic technologies have allowed investigation of viral infectivity, measurement of fusion kinetics, and monitoring of viral responses to neutralising compounds. In addition, microfluidic platforms represent promising new clinical tools with applications in diagnostics and drug screening. Summary Although the potential of microfluidics in virology is beginning to be realised, it has certainly not been fully explored. While not a replacement for macroscale investigative techniques, microfluidic platforms have the potential to be utilised alongside systems biology to provide novel methods of detailed virus study, with unique advantages.

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Section: Microfluidic Platforms For the Study Of Viral Cell Biology Amentioning

confidence: 88%

Microfluidics: an Untapped Resource in Viral Diagnostics and Viral Cell Biology

Simpson

Lee

et al. 2018

Curr Clin Micro Rpt

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“…A monomer of an elastomer such as PDMS (Karthik et al, 2015;Xu et al, 2012) is poured over a three-dimensional (3D) mask used as the (Hierholzer et al, 1969). A large number of patterning techniques are developed for the microfabrication of microfluidic devices, such as replica molding, microcontact printing, micromolding in capillaries, hot embossing, and microtransfer molding.…”

Section: Technology For Loc Fabricationmentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

195

146

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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“…For example, incorporating dielectrophoretic filters within microfluidic chips has enabled the capture of viral particles from a flowing system and their real-time imaging (Akin et al 2004), a useful technique for separating and concentrating viruses from mixed communities. Multi-layer devices have been employed to regulate the delivery of viruses to a host population and to image the infection dynamics in real time (Cimetta et al 2012, Xu et al 2012). …”

Section: A Dynamic Neighborhoodmentioning

confidence: 99%

Microfluidics Expanding the Frontiers of Microbial Ecology

Rusconi

Garren

Stocker

2014

Annu. Rev. Biophys.

177

146

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The ability afforded by microfluidics to observe the behaviors of microbes in highly controlled and confined microenvironments, across scales from a single cell to mixed communities, has significantly contributed to expand the frontiers of microbial ecology over the last decade. Spatially and temporally varying distributions of organisms and chemical cues that mimic natural microbial habitats can now be established by exploiting physics at the micrometer scale and by incorporating structures with specific geometries and materials. Here we review applications of microfluidics that have resulted in highly insightful discoveries on fundamental aspects of microbial life, ranging from growth and sensing to cell-cell interactions and population dynamics. We anticipate that this flexible, multidisciplinary technology will continue to facilitate discoveries regarding the ecology of microorganisms and help uncover strategies to control phenomena such as biofilm formation and antibiotic resistance.

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A microfluidic platform for real-time and in situ monitoring of virus infection process

Cited by 21 publications

References 38 publications

Microfluidics: an Untapped Resource in Viral Diagnostics and Viral Cell Biology

Microfluidics: an Untapped Resource in Viral Diagnostics and Viral Cell Biology

Recent advances in lab-on-a-chip technologies for viral diagnosis

Microfluidics Expanding the Frontiers of Microbial Ecology

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