A microfluidic platform integrated with field-effect transistors for enumeration of circulating tumor cells

Chen, Yihong; Pulikkathodi, Anil Kumar; Ma, Yu-Dong; Wang, Yulin; Lee, Gwo‐Bin

doi:10.1039/c8lc01072b

Cited by 47 publications

(37 citation statements)

References 45 publications

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“…The numbered microscope slide was fabricated using an SU‐8 standard photolithography process [ 44 ] to facilitate CTC counting. Concisely, a 30 µm thick layer of SU‐8 3035 (MicroChem, USA) was spin‐coated on a glass microscope slide.…”

Section: Methodsmentioning

confidence: 99%

Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

Gopinathan

Chiang

Bandaru

et al. 2020

Adv Healthcare Materials

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The search of alternative approaches to epithelial cell adhesion molecule (EpCAM), for the isolation of circulating tumor cells (CTC), is on the rise. This work attempts at evaluating the feasibility of using a new glycosaminoglycan, SCH45, as a probe to isolate CTCs from the peripheral blood of 65 advanced/metastatic cholangiocarcinoma (CCA) patients. The positive enrichment of CTCs from 1 mL of blood using SCH45‐bound magnetic beads and subsequent staining on an integrated microfluidic platform is demonstrated. Results detailing CTC concentrations averaging ≥1 CTCs mL−1 of blood are shown, and a conventional protein biomarker, EpCAM, has been used to corroborate the finding that 100% of the patients possess CTCs in their blood. Studies detailing the use of CTCs in the prognostic monitoring and treatment effectiveness of advanced/metastatic CCA are scarce, and the isolation of CTCs from all CCA patients tested has not been reported yet. A strong correlation between CTC counts and disease progression at the time of and/or in advance of radiographic imaging in patients receiving chemotherapy is also reported. This study is one of its kind with the new probe and reduced sample volume and has potential for use in CCA diagnosis and prognosis in the near future.

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Section: Methodsmentioning

confidence: 99%

Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

Gopinathan

Chiang

Bandaru

et al. 2020

Adv Healthcare Materials

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“…Besides, only the target cell that binds to specific aptamers on the FET sensor can be tested (Figure 3d), thus, this design prevents the interference of other blood cells. [68] A microchip for single-cell separation was designed by Wang et al according to the cell size and immune-phenotype. A large The diagram of the main program of the microfluidic system for multiple detections.…”

Section: High Precision Devicementioning

confidence: 99%

Advanced Devices for Tumor Diagnosis and Therapy

Wang

Dai

Lan

et al. 2021

Small

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Chinese mythology says that in the time of the Chou Dynasty ten suns were present in the sky, leading to a hard life for people because of the enormous heat. A hero named "Houyi" At present, tumor diagnosis is performed using common procedures, which are slow, costly, and still presenting difficulties in diagnosing tumors at their early stage. Tumor therapeutic methods also mainly rely on large-scale equipment or non-intelligent treatment approaches. Thus, an early and accurate tumor diagnosis and personalized treatment may represent the best treatment option for a successful result, and the efforts in finding them are still in progress and mainly focusing on non-destructive, integrated, and multiple technologies. These objectives can be achieved with the development of advanced devices and smart technology that represent the topic of the current investigations. Therefore, this review summarizes the progress in tumor diagnosis and therapy and briefly explains the advantages and disadvantages of the described microdevices, finally proposing advanced micro smart devices as the future development trend for tumor diagnosis and therapy.was sent from heaven to shoot down nine suns, leaving only the one that is still shining today to allow a normal life for people (Scheme 1). In real life, cancer threatens people's lives like the nine suns in mythology. The history of the struggle between human beings and tumors is represented by the development of science and technology. The diagnosis of diseases in ancient China by the Chinese medicine used the approach of "observing, listening, asking, and pulse feeling". Observing is referred to as the general inspection of the body and tongue inspection. Listening is referred to listening to sounds and smelling odor. Asking is referred to ask the symptoms. Pulse feeling represents the use of fingers to evaluate the radial artery pulsation behind the wrist, and it represents the essence of the diagnosis by traditional Chinese medicine. It is also the most difficult one among the four diagnostic methods because doctor should determine which organs have lesions from the pulsation changes, which is difficult to perceive by ordinary people. Therefore, without any anatomy support, pulse feeling in the diagnosis of incurable diseases has very large limitations. Pulse feeling can be replaced by intuitive diagnostic techniques in modern medicine. Indeed, tumors are diagnosed in various ways thanks to the development of modern science and technology, and the ever-evolving therapy technology is also transformed into an "arrow of Houyi" to shoot the "poisonous sun" that threatens human health.A timely and accurate diagnosis of cancer is crucial for choosing the correct treatment plan for a consequent successful cure. Usually, the methods to diagnose tumors include imaging, endoscopic investigation, and diagnostic pathology. Pathology techniques are important in clinical diagnosis, but they require biopsy and cannot be monitored in real time. Endoscopic technology allows a direct tumor monitori...

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“…The chip was tested with human colon cancer cell lines (HCT-8), as a CTC model and blood samples spiked with HCT-8 cells. The device was able to capture a maximum of 42 from a total of 1000 cancer cells [57].…”

Section: Platforms Based On Circulating Tumor Cells Analysismentioning

confidence: 99%

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Sierra

Marrugo-Ramírez

Rodriguez-Trujíllo

et al. 2020

Sensors

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Cancer represents one of the conditions with the most causes of death worldwide. Common methods for its diagnosis are based on tissue biopsies—the extraction of tissue from the primary tumor, which is used for its histological analysis. However, this technique represents a risk for the patient, along with being expensive and time-consuming and so it cannot be frequently used to follow the progress of the disease. Liquid biopsy is a new cancer diagnostic alternative, which allows the analysis of the molecular information of the solid tumors via a body fluid draw. This fluid-based diagnostic method displays relevant advantages, including its minimal invasiveness, lower risk, use as often as required, it can be analyzed with the use of microfluidic-based platforms with low consumption of reagent, and it does not require specialized personnel and expensive equipment for the diagnosis. In recent years, the integration of sensors in microfluidics lab-on-a-chip devices was performed for liquid biopsies applications, granting significant advantages in the separation and detection of circulating tumor nucleic acids (ctNAs), circulating tumor cells (CTCs) and exosomes. The improvements in isolation and detection technologies offer increasingly sensitive and selective equipment’s, and the integration in microfluidic devices provides a better characterization and analysis of these biomarkers. These fully integrated systems will facilitate the generation of fully automatized platforms at low-cost for compact cancer diagnosis systems at an early stage and for the prediction and prognosis of cancer treatment through the biomarkers for personalized tumor analysis.

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A microfluidic platform integrated with field-effect transistors for enumeration of circulating tumor cells

Abstract: A new integrated microfluidic system was developed for automatic detection and enumeration of CTCs with field-effect transistors (FETs).

Cited by 47 publications

References 45 publications

Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform

Advanced Devices for Tumor Diagnosis and Therapy

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

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