2017
DOI: 10.1039/c7lc00763a
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A microfluidic trap array for longitudinal monitoring and multi-modal phenotypic analysis of individual stem cell aggregates

Abstract: Three-dimensional pluripotent stem cell (PSC) cultures have the ability to undergo differentiation, self-organization, and morphogenesis to yield complex, in vitro tissue models that recapitulate key elements of native tissues. These tissue models offer a system for studying mechanisms of tissue development, investigating disease mechanisms, and performing drug screening. It remains challenging, however, to standardize PSC aggregate differentiation and morphogenesis methods due to heterogeneity stemming from b… Show more

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Cited by 21 publications
(22 citation statements)
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References 56 publications
(49 reference statements)
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“…The method has been pioneered for and widely applied to the study of eukaryotic cells, exploiting either micro-wells structures acting as mechanical barriers 9,10,27 or the difference in hydraulic resistance between different fluidic paths to drive cells towards small openings. [28][29][30] However, it has not been applied as extensively to the study of single bacterial cells, with only a few examples available in the relevant literature. 31,32 For most of the aforementioned bacteria trapping platforms, growth is employed as a signature of susceptibility to antibiotics, either by direct imaging 4,16 or by indirect measurements such as fluorescence 21,22 or Raman analysis.…”
Section: Introductionmentioning
confidence: 99%
“…The method has been pioneered for and widely applied to the study of eukaryotic cells, exploiting either micro-wells structures acting as mechanical barriers 9,10,27 or the difference in hydraulic resistance between different fluidic paths to drive cells towards small openings. [28][29][30] However, it has not been applied as extensively to the study of single bacterial cells, with only a few examples available in the relevant literature. 31,32 For most of the aforementioned bacteria trapping platforms, growth is employed as a signature of susceptibility to antibiotics, either by direct imaging 4,16 or by indirect measurements such as fluorescence 21,22 or Raman analysis.…”
Section: Introductionmentioning
confidence: 99%
“…Recent efforts with droplet generators have incorporated a droplet trapping array downstream of the flow channel to facilitate dynamic high-throughput screening of single cells on-chip while minimizing the chance for contamination and decreasing operator influence [18]. Interesting recent examples include a microfluidic trap array developed by Jackson-Holmes and colleagues for analyzing individual stem cell aggregates [19] and a static droplet array developed by Jin and colleagues for analysis of cell-cell communication in a confined microenvironment [15]. Trapping arrays are highly beneficial due to enabling process integration and increasing the overall analytical throughput by confining cells in pre-defined patterns which significantly reduces the computational burden and the time required for both image collection and automated image processing.…”
Section: Introductionmentioning
confidence: 99%
“…Worm images on our database were derived from brightfield and darkfield microscope configurations, solid and liquid imaging environments, and included both processed andbinarized images as well as unprocessed frames from raw videos (Figure1B). Stem cell aggregate images on our database were derived from phase images of both live and fixed aggregates grown in tissue culture plates as well as aggregates grown in microfluidic devices [12] (Figure1C). For both nematode and stem cell aggregate applications, users are presented with randomized images from the full dataset and draw a single contour.…”
Section: Resultsmentioning
confidence: 99%