A Micromethod for the Determination of Uronic Acids

Burkhart, B. A.; Baur, Lorenz; Link, Karl Paul

doi:10.1016/s0021-9258(18)75804-3

Cited by 28 publications

(2 citation statements)

References 11 publications

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“…acetic acid was contained in the first two fractions. The uronic anhydride was determined by the method of Burkhart, Baur, and Link (10) and reducing sugars, after acid hydrolysis, by the Hagedorn-Jensen method (11). Viscosities were measured in 0.9 per cent salt solution with an Ostwald viscometer at 20°C.…”

Section: Chemical and Physical Properties Of Specific Polysaccharides Of Types I Ii And Iii Pneumococcus--the Chemical And Physical Propementioning

confidence: 99%

The Specific Polysaccharides of Types I, Ii, and Iii Pneumococcus

Heidelberger

Kendall

Scherp

1936

Journal of Experimental Medicine

120

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For some years it has become increasingly evident that the methods originally proposed (1) for the isolation of the soluble specific substance (S) of pneumococcus were in need of revision. Avery and Goebel (2) and Enders and Pappenheimer (3) showed that more cautious manipulation of Type I pneumococcus or its culture filtrate led to the isolation of a product chemically and immunologically different from S I as originally described. Avery and Goebel presented evidence that these differences were due to a labile acetyl group which was removed by the treatment with alkali called for in the original method (1). We therefore undertook the preparation of the specific polysaccharides of Types II and III pneumococcus (S II and S III) without the use of alkali, and found no chemical differences (4) from the preparations made as originally described.Avery and Goebel (2) had also shown qualitatively that the acetyl S I precipitated antibody from Type I antipneumococcus horse serum which the alkali-treated, deacetylated S I failed to throw down. Our quantitative determinations on a Type I antibody solution showed that the new product precipitated 2.0 rag. of antibody nitrogen as compared with 1.3 rag. thrown down by the deacetylated product. However, the S II and S I I I preparations which had not been alkali-treated failed to precipitate more antibody from homologous antipneumococcus horse serum than did the older preparations.

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Section: Chemical and Physical Properties Of Specific Polysaccharides Of Types I Ii And Iii Pneumococcus--the Chemical And Physical Propementioning

confidence: 99%

The Specific Polysaccharides of Types I, Ii, and Iii Pneumococcus

Heidelberger

Kendall

Scherp

1936

Journal of Experimental Medicine

120

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show abstract

“…Mannan in wood and in «cellulose were determined by a very slight modification of the method of Hágglund and Bratt (?). Uronic acid was determined by a modification of the method of Burkart, Baur, and Link (3). This modification involved heating the sample with acid at 70°C.…”

Section: Analysis Of Pulpwoodsmentioning

confidence: 99%