A micropit for biological cell positioning

Clow, Andrew L.; Gaynor, Paul; Oback, Björn

doi:10.1109/icca.2009.5410507

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…In general, a microelectrode for DEP is fabricated by photolithography, laser ablation, MEMS technique and so on [8][9][10]. However, these methods spend much money and need many processes.…”

Section: Fabrication Of the Measurement Chipmentioning

confidence: 99%

Cellular Temperature Measurement by Dielectrophoretic Impedance Measurement Method

Kido

Taguchi

2015

Journal of the Japan Society of Applied Electromagnetics and Me

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Dielectrophoretic impedance measurement (DEPIM) method has recently attracted attention because the method is simple and immediately. We demonstrated that the impedance between the electrodes with trapped cell has thermal property and measured cellular temperature by the impedance change even if the number of trapped cells did not change. However, this result was not observed when cells were not trapped between the electrodes. In summary, the cellular temperature could be measured by observing the change in shunt voltage. In this study, we developed cellular temperature measurement system and it was expected to be used in biosensor application. In the future, we want to measure single cellular temperature with isolation by optical tweezers.

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Section: Fabrication Of the Measurement Chipmentioning

confidence: 99%

Cellular Temperature Measurement by Dielectrophoretic Impedance Measurement Method

Kido

Taguchi

2015

Journal of the Japan Society of Applied Electromagnetics and Me

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“…In medical field, it may be possible to use this method to test whether the cell is normal or not with little amount of sample. In general, a micro electrode of DEP is fabricated by photolithography or laser ablation and so on [3,4]. These methods are costly and need complex processes.…”

Section: Introductionmentioning

confidence: 99%

Cell Culture and Trapping of Yeast Protoplasts Using Au Thin-Film Dielectrophoresis Chip

Nishimoto¹,

Taguchi²,

Aritoshi³

2015

Proceedings of the 2015 International Conference on Electrical, Automation and Mechanical Engineering

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Dielectrophoresis (DEP) force will arise when an inhomogeneous AC electric field with sinusoidal wave form is applied to micro electrodes. The DEP is able to distinguish between viable and non-viable biological cells by their movement through a non-uniform electric field. The viable cells can be used for analytic chemistry, cell culture or fusion. The aim of this research is yeast cell culture after distinction of viable and nonviable cells on Au thin-film DEP chip. We fabricated a planar double pole electrode for DEP using Au thin-film and a box cutter. This fabrication method is low cost and simpler than the previous existing methods. We observed cell culture under the terms of DEP (1Vpp-5MHz) after distinction between viable and non-viable yeast cells. The Au thin-film DEP chip could obtain viable cells which were trapped at the micro electrodes. The daughter cell became as big as the mature cells after about 50 minutes of observation. In other experiments, we confirmed whether the Au thin-film DEP chip can trap yeast protoplasts or not. When we applied 4Vpp and over 40kHz, the yeast protoplasts were trapped at the micro electrode. This experimental result is very important for performing fusion of cells. We confirmed usefulness and possibility of Au thin-film DEP chip through these experiments.

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“…DEP is promising for identification of abnormal cells, which cause cancer, and normal cells. In general, microelectrodes used for DEP are fabricated by photolithography or laser ablation and so on [4,5]. These methods are costly and complex processes.…”

Section: Introductionmentioning

confidence: 99%

Combination of Au Dielectrophoresis Chip and Optical Tweezers for Cell Culture

Nishimoto

Taguchi

2015

KEM

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Dielectrophoresis (DEP) force will arise when an inhomogeneous AC electric field with sinusoidal wave is applied to microelectrodes. By using DEP, we could distinguish between viable and non-viable cells by their movement through a non-uniform electric field. In this paper, we propose a yeast cell separation system, which utilizes an Au DEP chip and an optical tweezers. The Au DEP chip is planar quadrupole microelectrodes, which were fabricated by Au thin-film and a box cutter. This fabrication method is low cost and simpler than previous existing methods. The tip of the optical tweezers was fabricated by dynamic chemical etching in a mixture of hydrogen fluoride and toluene. The optical tweezers has the feature of high manipulation performance. That does not require objective lens for focusing light because the tip of optical tweezers has conical shape. By using both the Au DEP chip and optical tweezers, we could obtain three-dimensional manipulation of specific cells after viability separation.

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A micropit for biological cell positioning

Cited by 6 publications

References 18 publications

Cellular Temperature Measurement by Dielectrophoretic Impedance Measurement Method

Cellular Temperature Measurement by Dielectrophoretic Impedance Measurement Method

Cell Culture and Trapping of Yeast Protoplasts Using Au Thin-Film Dielectrophoresis Chip

Combination of Au Dielectrophoresis Chip and Optical Tweezers for Cell Culture

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