A microRNA profile inFmr1knockout mice reveals microRNA expression alterations with possible roles in fragile X syndrome

Abstract

Fragile X syndrome (FXS), a common form of inherited mental retardation, is caused by a loss of expression of the fragile X mental retardation protein (FMRP). FMRP is involved in brain functions by interacting with mRNAs and microRNAs (miRNAs) that selectively control gene expression at translational level. However, little is known about the role of FMRP in regulating miRNA expression. Here, we found a development-dependant dynamic expression of Fmr1 mRNA (encoding FMRP) in mouse hippocampus with a small peak at postnatal day 7 (P7). MiRNA microarray analysis showed that the levels of 38 miRNAs showed a significant increase with about 15∼250 folds and the levels of 26 miRNAs showed a significant decrease with only about 2∼4 folds in the hippocampus of P7 Fmr1 KO mice. Q-PCR assay showed that 9 of the most increased miRNAs (>100 folds in microarrays) were increased about 40∼70 folds and their pre-miRNAs were increased about 5∼10 folds, but no significant difference in their pri-miRNA levels was observed, suggesting a role of FMRP in regulating miRNA processing from pri-miRNA to pre-miRNA. We further demonstrated that a set of protein-coding mRNAs, potentially targeted by the 9 miRNAs were down-regulated in the hippocampus of Fmr1 KO mice. Finally, luciferase assays demonstrated that miR-34b, miR-340, miR-148a could down-regulate the reporter gene expression by interacting with the Met 3′ UTR. Taken together these findings suggest that the miRNA expression alterations resulted from the absence of FMRP might contribute to molecular pathology of FXS.

A MicroRNA Profile in Fmr1 Knockout Mice Reveals MicroRNA Expression Alterations with Possible Roles in Fragile X Syndrome

“…Our results show a development-dependant dynamic expression of Fmr1 mRNA in mouse hippocampus with a peak at P7, suggesting an important role of FMRP in the hippocampal development and function. The P7 KO mouse, corresponding to the beginning of human full-term infant (Romijn et al 1991), is accepted as a model for the FXS (Bilousova et al 2009), which is the reason why we investigated the miRNA expression profile in the hippocampus of P7 Fmr1 KO mouse certified by peer review) is the author/funder. All rights reserved.…”