A microsatellite-based genetic linkage map and putative sex-determining genomic regions in Lake Victoria cichlids

Kudo, Yuji; Nikaido, Masato; Kondo, Akiko; Suzuki, Haruna; Yoshida, Kohta; Kikuchi, Kiyoshi; Okada, Norihiro

doi:10.1016/j.gene.2015.01.057

Cited by 13 publications

(24 citation statements)

References 59 publications

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“…Maintaining the community naming convention based on the original O. niloticus linkage map, this linkage group would be given the rather cumbersome designation LG8-24-16-21. A previous report suggested that one of the fusions in A. burtoni consisted of LG15 and LG19 relative to other cichlids [31], but we find no evidence of linkage between markers mapping to those linkage groups (LOD = 0.8, Additional file 4). This discrepancy may stem from interchromosomal rearrangements more complex than simple fusions, where different portions of a particular tilapia LG map to different A. burtoni LGs.…”

Section: Resultscontrasting

confidence: 72%

“…In Lake Malawi haplochromine cichlid species, LG3, LG5, LG20, and two loci on LG7 have been associated with sex determination [21, 23]. In haplochromine cichlids from Lake Victoria, LG2, LG5, and supernumerary B chromosomes have been associated with sex [31, 32]. Here we identify two loci impacting sex determination in the riverine haplochromine A. burtoni, LG5-14 and LG13.…”

Section: Resultsmentioning

confidence: 88%

“…Colored bars indicate range of sex-associated markers relative to A. burtoni scaffolds above in a . Kudo et al [31] described association with sex without indicating an XY or ZW system. Roberts et al [21] mapped the orange blotch (OB) pigmentation allele, tightly linked to a W sex determination allele …”

Section: Resultsmentioning

confidence: 99%

“…These sex determination loci exist in various contexts, including polygenic sex determination [22, 23, 30, 31], on supernumerary chromosomes [32], and in regions of potential recombination suppression indicative of early sex chromosome evolution [21, 36, 37]. The riverine cichlid Astatotilapia burtoni has served as a longstanding model of sexual physiology, behavior, and related gene expression [38], yet until recently sex determination in the species was largely unexamined.…”

Section: Introductionmentioning

confidence: 99%

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Polygenic sex determination in the cichlid fish Astatotilapia burtoni

et al. 2016

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BackgroundThe East African riverine cichlid species Astatotilapia burtoni serves as an important laboratory model for sexually dimorphic physiology and behavior, and also serves as an outgroup species for the explosive adaptive radiations of cichlid species in Lake Malawi and Lake Victoria. An astounding diversity of genetic sex determination systems have been revealed within the adaptive radiation of East African cichlids thus far, including polygenic sex determination systems involving the epistatic interaction of multiple, independently segregating sex determination alleles. However, sex determination has remained unmapped in A. burtoni. Here we present mapping results supporting the presence of multiple, novel sex determination alleles, and thus the presence of polygenic sex determination in A. burtoni.ResultsUsing mapping in small families in conjunction with restriction-site associated DNA sequencing strategies, we identify associations with sex at loci on linkage group 13 and linkage group 5–14. Inheritance patterns support an XY sex determination system on linkage group 5–14 (a chromosome fusion relative to other cichlids studied), and an XYW system on linkage group 13, and these associations are replicated in multiple families. Additionally, combining our genetic data with comparative genomic analysis identifies another fusion that is unassociated with sex, with linkage group 8–24 and linkage group 16–21 fused in A. burtoni relative to other East African cichlid species.ConclusionsWe identify genetic signals supporting the presence of three previously unidentified sex determination alleles at two loci in the species A. burtoni, strongly supporting the presence of polygenic sex determination system in the species. These results provide a foundation for future mapping of multiple sex determination genes and their interactions. A better understanding of sex determination in A. burtoni provides important context for their use in behavioral studies, as well as studies of the evolution of genetic sex determination and sexual conflicts in East African cichlids.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3177-1) contains supplementary material, which is available to authorized users.

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Section: Resultscontrasting

confidence: 72%

Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polygenic sex determination in the cichlid fish Astatotilapia burtoni

et al. 2016

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“…In the mbuna cichlids from Lake Malawi (LM), an XX-XY system has been mapped to LG7, whereas a ZZ-ZW system in the same clade maps to LG5 [13]. Sex in two cichlid species from Lake Victoria (LV) has been mapped to a different region also on LG5 [8]. In the tilapiine lineage (basal to the lake radiations), which feature male and female heterogametic systems with additional ESD to some extent, sex-associated chromosomes include LGs 1, 3 and 23 [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

et al. 2016

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BackgroundSex chromosomes change more frequently in fish than in mammals or birds. However, certain chromosomes or genes are repeatedly used as sex determinants in different members of the teleostean lineage. East African cichlids are an enigmatic model system in evolutionary biology representing some of the most diverse extant vertebrate adaptive radiations. How sex is determined and if different sex-determining mechanisms contribute to speciation is unknown for almost all of the over 1,500 cichlid species of the Great Lakes. Here, we investigated the genetic basis of sex determination in a cichlid from Lake Tanganyika, Astatotilapia burtoni, a member of the most species-rich cichlid lineage, the haplochromines.ResultsWe used RAD-sequencing of crosses for two populations of A. burtoni, a lab strain and fish caught at the south of Lake Tanganyika. Using association mapping and comparative genomics, we confirmed male heterogamety in A. burtoni and identified different sex chromosomes (LG5 and LG18) in the two populations of the same species. LG5, the sex chromosome of the lab strain, is a fusion chromosome in A. burtoni. Wnt4 is located on this chromosome, representing the best candidate identified so far for the master sex-determining gene in our lab strain of A. burtoni.ConclusionsCichlids exemplify the high turnover rate of sex chromosomes in fish with two different chromosomes, LG5 and LG18, containing major sex-determining loci in the two populations of A. burtoni examined here. However, they also illustrate that particular chromosomes are more likely to be used as sex chromosomes. Chromosome 5 is such a chromosome, which has evolved several times as a sex chromosome, both in haplochromine cichlids from all Great Lakes and also in other teleost fishes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3178-0) contains supplementary material, which is available to authorized users.

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Genetic sex determination in Astatotilapia calliptera, a prototype species for the Lake Malawi cichlid radiation

Peterson

Cline

Moore

et al. 2017

Sci Nat

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East African cichlids display extensive variation in sex determination systems. The species Astatotilapia calliptera is one of the few cichlids that reside both in Lake Malawi and in surrounding waterways. A. calliptera is of interest in evolutionary studies as a putative immediate outgroup species for the Lake Malawi species flock and possibly as a prototype ancestor-like species for the radiation. Here, we use linkage mapping to test association of sex in A. calliptera with loci that have been previously associated with genetic sex determination in East African cichlid species. We identify a male heterogametic XY system segregating at linkage group (LG) 7 in an A. calliptera line that originated from Lake Malawi, at a locus previously shown to act as an XY sex determination system in multiple species of Lake Malawi cichlids. Significant association of genetic markers and sex produce a broad genetic interval of approximately 26 megabases (Mb) using the Nile tilapia genome to orient markers; however, we note that the marker with the strongest association with sex is near a gene that acts as a master sex determiner in other fish species. We demonstrate that alleles of the marker are perfectly associated with sex in Metriaclima mbenjii, a species from the rock-dwelling clade of Lake Malawi. While we do not rule out the possibility of other sex determination loci in A. calliptera, this study provides a foundation for fine mapping of the cichlid sex determination gene on LG7 and evolutionary context regarding the origin and persistence of the LG7 XY across diverse, rapidly evolving lineages.

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A microsatellite-based genetic linkage map and putative sex-determining genomic regions in Lake Victoria cichlids

Cited by 13 publications

References 59 publications

Polygenic sex determination in the cichlid fish Astatotilapia burtoni

Polygenic sex determination in the cichlid fish Astatotilapia burtoni

Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni

Genetic sex determination in Astatotilapia calliptera, a prototype species for the Lake Malawi cichlid radiation

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