A mini review of xylanolytic enzymes with regards to their synergistic interactions during hetero-xylan degradation

Malgas, Samkelo; Mafa, Mpho S.; Mkabayi, Lithalethu; Pletschke, Brett I.

doi:10.1007/s11274-019-2765-z

Cited by 94 publications

(78 citation statements)

References 101 publications

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“…A number of enzymes are required to completely degrade the heterogeneous network of WB polysaccharides [ 27 ]. Synergism between CELase, XYLase, β -xylosidase, AFase, FEase, and α -D-glucuronosidases is vital for efficient polysaccharide backbone degradation and production of target nutraceutical compounds.…”

Section: Discussionmentioning

confidence: 99%

Enzyme Selection and Hydrolysis under Optimal Conditions Improved Phenolic Acid Solubility, and Antioxidant and Anti-Inflammatory Activities of Wheat Bran

et al. 2020

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Valorization of wheat bran (WB) into new high-value products is of great interest within the framework of sustainability and circular economy. In the present study, we utilized a multi-step approach to extract nutraceutical compounds (phenolic acids) from WB and improved its antioxidant and anti-inflammatory properties through using sequential hydrothermal and enzymatic hydrolysis. Thirteen commercial glycosidases differing in their specific activity were screened and compared for hydrolytic efficiency to release monosaccharides, ferulic acid, and diferulic acid. Ultraflo XL was selected as the desired enzyme treatment on the basis of its higher WB solubilization, as well as its monosaccharide and phenolic acids yields. The relationships between better hydrolytic performance of Ultraflo XL and its particular activity profile were established. To determine the optimum conditions for Ultraflo XL treatment, we tested different factors (solvent pH, incubation temperature, and time) under 15 experiments. A multicomponent analysis (MCA), including central composite design, model fitness, regression coefficients, analysis of variance, 3D response curves, and desirability, was used for processing optimization. A beneficial effect of autoclave treatment on the release of phenolic compounds was also evidenced. The results of MCA showed involvement of linear, quadratic, and interactive effects of processing factors, although solvent pH was the main determinant factor, affecting enzymatic extraction of phenolics and bioactivity of hydrolysates. As compared to control WB, under optimized conditions (47 °C, pH = 4.4, and 20.8 h), WB hydrolysates showed 4.2, 1.5, 2, and 3 times higher content of ferulic acid (FA) and capacity to scavenge oxygen radicals, chelate transition metals, and inhibit monocyte chemoattractant protein-1 secretion in macrophages, respectively. These approaches could be applied for the sustainable utilization of WB, harnessing its nutraceutical potential.

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Section: Discussionmentioning

confidence: 99%

Enzyme Selection and Hydrolysis under Optimal Conditions Improved Phenolic Acid Solubility, and Antioxidant and Anti-Inflammatory Activities of Wheat Bran

et al. 2020

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“…Renewable resource lignocellulose consists of cellulose (40%-60%) and hemicellulose (20-40%) [1], which shows signi cant importance for bioresource conversion and sustainable development. The biodegradation of lignocellulose for biofuels production needs the enzymatical hydrolysis that was mainly functioned by cellulase.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the degradation of lignocellulose needs the synergetic work of cellulase and xylanase. Xylan is mainly composed of -xylose, which is linked with β-1,4-glycosidic bonds, and other -galactose and L-arabinose substituent groups, making a complex hetero-polymeric structure [1]. The biodegradation of xylan requires a complex enzyme cocktail that is commonly named xylanase [5].…”

Section: Introductionmentioning

confidence: 99%

“…The biodegradation of xylan requires a complex enzyme cocktail that is commonly named xylanase [5]. Xylanase is a group of xylanolytic enzymes consisting of endo-xylanase, β-xylosidase, α-L-arabinofuranosidase, acetyl xylan esterase and ferulic acid esterase, which decompose xylan into simple monosaccharide and xylooligosaccharides [1,6], showing great potential in feed, biore nery and pulp paper industry. Generally, the recombinant production of xylanase was achieved by overexpression of endo-xylanases, which shows great speci city to the backbone of xylan.…”

Section: Introductionmentioning

confidence: 99%

“…Generally, the recombinant production of xylanase was achieved by overexpression of endo-xylanases, which shows great speci city to the backbone of xylan. While the insu cient cleave of xylobiose into xylose and the unthorough hydrolysis of the sub-chain group also hinder the thorough degradation of xylan [1], resulting in low fermentable sugar for downstream biofuels production. Thus, xylanase cocktail exhibits more potential for e cient biomass degradation, compared with addition of single kind of xylanase.…”

Section: Introductionmentioning

confidence: 99%

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Rational Engineering A Xylanase Hyper-Producing System in Trichoderma Reesei for Efficient Biomass Degradation

Xiang

2020

Preprint

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Abstract Background: The degradation of lignocellulose needs the synergetic work of cellulase and xylanase. Filamentous fungi Trichoderma reesei has been widely used as a workhorse for cellulase and xylanase fermentation with great producing ability. Previous work for biomass saccharification mainly aims to improve the component and production of cellulase, however the low xylanase activity in T. reesei could not meet the needs of a high saccharification efficiency. Results: In this study, for the first time, a xylanase hyper-producing system in T. reesei was established by tailoring two transcription factors, XYR1 and ACE1, and homologous over-expression of the major xylanase XYNⅡ. The expression of key xylanolytic enzymes was significantly upregulated with an increase of 6.78- and 1.98-fold in the xylanase activity and pNPXase (β-xylosidase) activity compared to that of the parent, Rut-C30, respectively. Besides, 2310-3085 U/mL of xylanase activities were achieved using soluble lactose or glucose as sole carbon source, which was more efficient and economical than the traditional method of xylan induction. Treated with the crude xylanase cocktails as accessory enzymes, an increase of 39.7% in reducing sugar yield (31.3 mg/mL) in saccharification of alkali pretreated corn stover (5% w/v) was achieved compared to that of the parent. Conclusions: An efficient and economical xylanase hyper-producing platform was developed in T. reesei Rut-C30. The novel platform with outstanding ability for crude xylanase cocktails production would greatly fit in biomass degradation and give a new perspective of further engineering in T. reesei for industrial purposes.

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Enzymes for Hemicellulose Degradation

Bankeeree¹,

Prasongsuk²,

Lotrakul³

et al. 2022

Biorefinery of Oil Producing Plants for Value‐Added Products

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A mini review of xylanolytic enzymes with regards to their synergistic interactions during hetero-xylan degradation

Cited by 94 publications

References 101 publications

Enzyme Selection and Hydrolysis under Optimal Conditions Improved Phenolic Acid Solubility, and Antioxidant and Anti-Inflammatory Activities of Wheat Bran

Enzyme Selection and Hydrolysis under Optimal Conditions Improved Phenolic Acid Solubility, and Antioxidant and Anti-Inflammatory Activities of Wheat Bran

Rational Engineering A Xylanase Hyper-Producing System in Trichoderma Reesei for Efficient Biomass Degradation

Enzymes for Hemicellulose Degradation

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