A Miniature Neural Recording Device to Investigate Sleep and Temperature Regulation in Mice

Abstract

Sleep is an important and ubiquitous process that, despite decades of research, a large part of its underlying biological circuity still remain elusive. To conduct research in this field, many devices capable of recording neural signals such as LFP and EEG have been developed. However, most of these devices are unsuitable for sleep studies in mice, the most commonly used animals, due to their size and weight. Thus, this paper presents a novel 4 channel, compact (2.1cm by 1.7cm) and lightweight (3.6g) neural-logging device that can record for 3 days on just two 0.6g zinc air 312 batteries. Instead of the typical solution of using multiple platforms, the presented device integrates high resolution EEG, EMG and temperature recordings into one platform. The onboard BLE module allows the device to be controlled wirelessly as well as stream data in real time, enabling researchers to check the progress of the recording with minimal animal disturbance. The device demonstrates its ability to accurately record EEG and temperature data through the long 24 hour in-vivo recordings conducted. The obtained EEG data could be easily sleep scored and the temperatures values were all within expected physiological range.

Brain clearance is reduced during sleep and anesthesia

“…EEG and EMG signals were recorded using a miniature datalogger attached to the skull 34 . The data were downloaded and waveforms https://doi.org/10.1038/s41593-024-01638-y visualized using MATLAB (MathWorks).…”

“…Policy information about availability of computer code Data collection EEG/EMG data was collected with a customized portable recording device (Hsieh, B. et al 2019). Photometry data was collected with Doric For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.…”